Author Watanabe, Shinsaku|
Published Date 1969-12
Publication Title Acta Medicinae Okayama
Volume volume23
Issue issue6
Content Type Journal Article
JaLCDOI 10.18926/AMO/31534
FullText URL fulltext.pdf
Author Watanabe, Shinsaku| Yamamoto, Ryosuke| Ogata, Masana| Murakami, Takuro|
Abstract <p>Erythrocytes in human blood stored for 120 days were collected by centrifugation after dispersion in buffered physiological saline. The aged erythrocytes thus collected were incubated with inosine, adenine, glucose or other media, and their shapes and ATP levels were studied by scanning electron microscopy and a luciferine-luciferase method. The aged erythrocytes incubated in a mixture of adenine and inosine markedly regained their ATP levels, and also showed a marked transformation from spiked spherocytes to normal discocytes. Incubation with inosine alone restored ATP levels of the aged erythrocytes to some extent, but did not result in morphological rejuvenation. Incubation in a mixture of citrate and glucose caused morphological rejuvenation, though it restored ATP levels less effectively than incubation in inosine alone. Incubation with adenine alone neither restored ATP levels nor resulted in morphological rejuvenation of the stored erythrocytes.</p>
Keywords human erythrocytes rejuvenation adenine inosine ATP Iuciferineluciferase method scanning electron microscopy discocyte transformation
Amo Type Article
Published Date 1985-06
Publication Title Acta Medica Okayama
Volume volume39
Issue issue3
Publisher Okayama University Medical School
Start Page 239
End Page 246
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4024994
Web of Sience KeyUT A1985ALG3300010
JaLCDOI 10.18926/AMO/31285
FullText URL fulltext.pdf
Author Watanabe, Shinsaku| Ogata, Masana|
Abstract <p>Generation of superoxide and hydrogen peroxide during interaction of nitrite with human hemoglobin was detected by chemiluminescence of luminol. Luminol chemiluminescence was inhibited by the addition of superoxide dismutase (SOD) and catalase. Methemoglobin formation induced by nitrite was also inhibited by the addition of SOD and catalase. The mechanism of methemoglobin formation by nitrite was discussed in regard to the oxidation of hemoglobin by superoxide and hydrogen peroxide as generated by the interaction of nitrite with hemoglobin.</p>
Keywords nitrite chemiluminescence methemoglobin superoxide dismutase.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 173
End Page 178
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6270983
Web of Sience KeyUT A1981MD16600003
JaLCDOI 10.18926/AMO/30666
FullText URL fulltext.pdf
Author Watanabe, Shinsaku| Ogata, Masana|
Abstract <p>Paraquat is a broad spectrum herbicide known to be highly lethal to man and animals. Its toxicity is characterized by acute lung injury. Paraquat produces such toxic effects through the generation of the superoxide anion according to one proposed mechanism. The present experiment, methemoglobin formation was demonstrated after incubation of oxyhemoglobin with paraquat. The generation of the superoxide anion through the interaction of oxyhemoglobin with paraquat was suggested by chemiluminescence of luminol. Superoxide dismutase (SOD) and catalare inhibited methemoglobin formation. The generation of the superoxide anion is discussed in regard to methemoglobin formation by paraquat.</p>
Keywords paraquat superoxide anion methmoglobin superoxide dismutase methemoglobin formation by paraquat
Amo Type Brief Note
Published Date 1982-12
Publication Title Acta Medica Okayama
Volume volume36
Issue issue6
Publisher Okayama University Medical School
Start Page 495
End Page 499
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6297260
Web of Sience KeyUT A1982PW07500010