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ID 17114
Eprint ID
17114
FullText URL
Thumnail 92_923.pdf 4.63 MB
Title Alternative
Molecular forms of SV40 chromatin and their functional changes
Author
Watanabe, Sekiko
Abstract
Simian virus 40 nucleoprotein complexes (SV40 chromatin) have a nucleosome structure similar to that of cellular chromatin and serve as a useful model system for studying structure and function of cellular chromatin. It is the purpose of this study to clarify the molecular forms of SV40 chromatin and their functional changes. African green monkey kidney cells (CV-1) were infected with SV40 and bulk or pulse-labeled with [(3)H] thymidine. SV40 chromatin was isolated under various conditions from these cell nuclei and examined by electron microscopy. When SV40 chromatin was extracted with a hypertonic salt-Triton X-100 solution from the nuclei isolated by treatment with a hypotonic ethylenediaminetetraacetic acid (EDTA) solution, the majority of SV40 chromatin molecules had a relaxed form showing a typical bead-like structure of nucleosomes. Most of these molecules were found to be derived from disrupted virions. When the extraction was performed under mild conditions in a near isotonic solution by no pretreatment with EDTA, SV40 chromatin and virions were separated in different fractions by sucrose density gradient centrifugation. The majority of these SV40 chromatin molecules had a condensed form. These molecules can be relaxed through various intermediate forms by treatment with a hypotonic or 0.6M NaCl solution. Replicating SV40 chromatin was sedimented faster than mature SV40 chromatin and showed the relaxed form. SV40 chromatin isolated from purified virion by alkali treatment showed the relaxed form. The relaxed molecules were condensed and their template activity for transcription was strongly inhibited by binding with histone Hl.
Keywords
SV40 クロマチン
ヌクレオソーム構造
分子形態
複製形
転写調節
Published Date
1980-08-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume92
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
923
End Page
941
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/92/7-8/92_7-8_923/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma