JaLCDOI 10.18926/AMO/31944
FullText URL fulltext.pdf
Author Higashi, Toshihiro| Hashimoto, Makoto| Watanabe, Masatomo| Yamauchi, Yasuhiko| Fujiwara, Masachika| Nakatsukasa, Harushige| Kobayashi, Michio| Watanabe, Akiharu|
Abstract <p>Cathepsin B, H and L activities in small amounts of rat tissue homogenates corresponding to 10 micrograms protein were determined with 7-amino-4-methyl-coumarin conjugates as substrates. A new procedure for serum cathepsin H activity was also developed. High cathepsin B and H activities were found in kidney, spleen and liver. Liver cathepsin B, H and L activities in D-galactosamine-injured rats were decreased concomitantly with an increase in serum cathepsin H activity.</p>
Keywords cathepsin B H and L serum cathepsin H liver injury.
Amo Type Article
Published Date 1986-02
Publication Title Acta Medica Okayama
Volume volume40
Issue issue1
Publisher Okayama University Medical School
Start Page 27
End Page 32
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3962728
Web of Sience KeyUT A1986A190200004
JaLCDOI 10.18926/AMO/31933
FullText URL fulltext.pdf
Author Kobayashi, Michio| Nakatsukasa, Harushige| Watanabe, Akiharu| Yamauchi, Yasuhiko| Fujiwara, Masachika| Hashimoto, Makoto| Watanabe, Masatomo| Higashi, Toshihiro| Nagashima, Hideo|
Abstract <p>&lt;p&gt;Type V collagen-degrading enzyme activity was detected as a metalloprotease acting at neutral pH in the human liver. Type V collagen extracted from human placenta and labeled with [1-14C] acetic anhydride was used as the substrate in the assay. Four major degradation products with relatively high molecular weights were observed upon polyacrylamide gel electrophoresis of the incubation mixture of type V collagen and liver homogenate. The significance of the measurement of this enzyme activity was discussed in relation to the clarification of the mechanism of liver fibrosis.&lt;/p&gt;</p>
Keywords type V collagen-degrading enzyme activity human liver liver fibrosis collagen degradation products
Amo Type Article
Published Date 1986-06
Publication Title Acta Medica Okayama
Volume volume40
Issue issue3
Publisher Okayama University Medical School
Start Page 179
End Page 182
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3017052
Web of Sience KeyUT A1986C914800007