| JaLCDOI | 10.18926/AMO/31323 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Wakimoto, Masahiro| Masuoka, Noriyoshi| Nakano, Taku| Ubuka, Toshihiko| |
| Abstract | <p>A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.</p> |
| Keywords | glutathione peroxidase erythrocyte hydrogen peroxide acatalasemic mouse Takahara disease |
| Amo Type | Article |
| Published Date | 1998-10 |
| Publication Title | Acta Medica Okayama |
| Volume | volume52 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 233 |
| End Page | 237 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9810432 |
| Web of Science KeyUT | 000076694300001 |
