JaLCDOI 10.18926/AMO/30504
FullText URL fulltext.pdf
Author Vibert-li, Jia-li| Okada, Shigeru|
Abstract <p>&#60;P&#62;We investigated the effect of estrogens, 17 beta-estradiol, estradiol-3-benzoate and estrone, on 2-amidinopropane hydrochloride (AAPH)-provoked, free radical-dependent hemolysis in vitro. Incubation experiment was performed by mixing AAPH (400 mM) and washed human erythrocyte suspension with or without various sex hormones and radical scavengers. After 170 min of incubation, 50% hemolysis was detected in the control group (incubation without sex hormones or radical scavengers), whereas after the addition of estrogens (5 mM), hemolysis was nearly completely inhibited until 180 min of incubation. It was found that the inhibitory activities of estrogens on oxidative hemolysis were stronger than that of alpha-tocopherol and had nearly identical to that of N-acetyl-L-cysteine. Testosterone had no inhibitory effects. The elevation of thiobarbituric acid-reactive substances, a marker for lipid peroxidation, was also inhibited by estrogens. These results add further evidence that estrogens are strong radical scavengers in humans.&#60;/P&#62;</p>
Keywords 2-amidinopropane hydrochioride (AAPH) oxidative hemolysis estrogens vitamin E N-acetyl-lcysteine
Amo Type Article
Published Date 1996-06
Publication Title Acta Medica Okayama
Volume volume50
Issue issue3
Publisher Okayama University Medical School
Start Page 125
End Page 130
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8805851
Web of Science KeyUT A1996UU60400002