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Author
Katashima, Kazunori
Ashida, Masakazu
Hara, Marina
Kato, Hirokazu
Ohmura, Yuichi
Kobayashi, Tomoki
Harada, Sosuke
Takemoto, Mitsuhiro
Ohno, Seiichiro
Mimura, Seiichi
Abstract
It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis.
Keywords
ADC
apoptosis
necrosis
hyperthermia
cell density
Amo Type
Original Article
Published Date
2013-12
Publication Title
Acta Medica Okayama
Volume
volume67
Issue
issue6
Publisher
Okayama University Medical School
Start Page
359
End Page
367
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
CopyrightⒸ 2013 by Okayama University Medical School
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publisher
Refereed
True
PubMed ID
Web of Sience KeyUT