このエントリーをはてなブックマークに追加
ID 48277
FullText URL
Author
Wajima, Takeaki
Sabui, Subrata
Fukumoto, Megumi
Kano, Shigeyuki
Ramamurthy, Thandavarayan
Chatterjee, Nabendu Sekhar
Hamabata, Takashi
Abstract
Enterotoxigenic Escherichia coli (ETEC) produces a variety of colonization factors necessary for attachment to the host cell, among which CS6 is one of the most prevalent in ETEC isolates from developing countries. The CS6 operon is composed of 4 genes, cssA, cssB, cssC, and cssD. The molecular mechanism of CS6 assembly and cell surface presentation, and the contribution of each protein to the attachment of the bacterium to intestinal cells remain unclear. In the present study, a series of css gene-deletion mutants of the CS6 operon were constructed in the ETEC genetic background, and their effect on adhesion to host cells and CS6 assembly was studied. Each subunit deletion resulted in a reduction in the adhesion to intestinal cells to the same level of laboratory E. coli strains, and this effect was restored by complementary plasmids, suggesting that the 4 proteins are necessary for CS6 expression. Bacterial cell fractionation and western blotting of the mutant strains suggested that the formation of a CssA–CssB–CssC complex is necessary for recognition by CssD and transport of CssA–CssB to the outer membrane as a colonization factor.
Keywords
Enterotoxigenic Escherichia coli
Colonization factor
CS6
Published Date
2011-10
Publication Title
Microbial Pathogenesis
Volume
volume51
Issue
issue4
Publisher
Elsevier
Start Page
243
End Page
249
ISSN
0882-4010
NCID
AA10609161
Content Type
Journal Article
Project
Collaborative Research of Okayama University for Infectious Diseases in India
Official Url
http://www.sciencedirect.com/science/article/pii/S0882401011001173
language
英語
Copyright Holders
©2011 Elsevier Ltd. All rights reserved.
File Version
author
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT