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ID 56699
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Ghosh, Priyanka Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Kumar, Dhirendra Maharishi Valmiki Infectious Diseases Hospital
Chowdhury, Goutam Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Singh, Puneeta Maharishi Valmiki Infectious Diseases Hospital
Samanta, Prosenjit Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Dutta, Shanta Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Ramamurthy, T. Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Sharma, N. C. Maharishi Valmiki Infectious Diseases Hospital
Sinha, Preety Department of Zoology, A.N. College
Prasad, Yogendra Department of Animal Science, MJP Rohilkhand University
Shinoda, Sumio Collaborative Research Center of Okayama University for Infectious Diseases at NICED
Mukhopadhyay, Asish K. Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Abstract
Vibrio cholerae O1 is the etiological agent of the severe diarrheal disease cholera. The bacterium has recently been causing outbreaks in Haiti with catastrophic effects. Numerous mutations have been reported in V. cholerae O1 strains associated with the Haitian outbreak. These mutations encompass among other the genes encoding virulence factors such as the pilin subunit of the toxin-co-regulated pilus (tcpA), cholera toxin B subunit (ctxB), repeat in toxins (rtxA), and other genes such as the quinolone resistance-determining region (QRDR) of gyrase A (gyrA), rstB of RS element along with the alteration in the number of repeat sequences at the promoter region of ctxAB. Given the numerous genetic changes in those Haitian isolates, we decided to investigate the possible origins of those variations in the Indian subcontinent. Thus, we determined the genetic traits among V. cholerae O1 strains in Delhi, India. A total of 175 strains isolated from cholera patients during 2004 to 2012 were analysed in the present study. Our results showed that all the tested strains carried Haitian type tcpA (tcpACIRS) and variant gyrA indicating their first appearance before 2004 in Delhi. The Haitian variant rtxA and ctxB7 were first detected in Delhi during 2004 and 2006, respectively. Interestingly, not a single strain with the combination of El Tor rtxA and ctxB7 was detected in this study. The Delhi strains carried four heptad repeats (TTTTGAT) in the CT promoter region whereas Haitian strains carried 5 such repeats. Delhi strains did not have any deletion mutations in the rstB like Haitian strains. Overall, our study demonstrates the sequential accumulation of Haitian-like genetic traits among V. cholerae O1 strains in Delhi at different time points prior to the Haitian cholera outbreak.
Keywords
Cholera
Vibrio cholerae
ctxAB promoter
ctxB
gyrA
rstB
rtxA
tcpA
Published Date
2017-10
Publication Title
Infection, Genetics and Evolution
Volume
volume54
Publisher
Elsevier Science
Start Page
47
End Page
53
ISSN
15671348
NCID
AA11697619
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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DOI
Web of Sience KeyUT
Related Url
isVersionOf https://doi.org/10.1016/j.meegid.2017.06.015
Project
Collaborative Research of Okayama University for Infectious Diseases in India