このエントリーをはてなブックマークに追加
ID 32834
JaLCDOI
FullText URL
Thumnail fulltext.pdf 1.48 MB
Author
Dirlik, Musa
Buyukafsar, Kansu
Cinel, Ismail
Cinel, Leyla
Caglikulekci, Mehmet
Tamer, Lulufer
Aydin, Suha
Oral, Ugur
Abstract

Effect of ornithine which is known to inhibit L-arginine uptake via cationic amino acid transport system has been tested, and compared to aminoguanidine, an iNOS inhibitor in lypopolysaccharide (LPS)-induced endotoxemia in rats. Serum nitrite/nitrate and malondialdehyde (MDA) level have been measured, and ileal histology has also been examined. Endotoxin increased serum nitrite/nitrate and MDA levels from 15.7+/- 2.4 micromol/ml and 2.1 +/-0.2 nmol/ml to 23.1 +/- 1.0 micromol/ml and 5.2+/- 0.3 nmol/ml (both P<0.05), respectively. In addition, LPS caused ileal degeneration. L-ornithine (500 mg/kg) did not improve septic manifestations, i.e., serum nitrite/nitrate and MDA levels did not differ from those in endotoxemia. Neither does it have an improving action on ileal histology. However, higher dose of L-ornithine (2,500 mg/kg) lowered the increased level of nitrite/nitrate and MDA by LPS. Moreover, it restored ileal histology from grade 3 (median) to 0 (median) (P<0.05). On the other hand, aminoguanidine (100 mg/kg) normalized serum nitrite/nitrate and MDA levels but not ileal histology in endotoxemic rats. In conclusion, high dose of L-ornithine could improve endotoxemic parameters in LPS-treated rats.

Keywords
LPS
ornithine
aminoguanidine
endotoxemia
lipid peroxidation
Amo Type
Article
Published Date
2003-06
Publication Title
Acta Medica Okayama
Volume
volume57
Issue
issue3
Publisher
Okayama University Medical School
Start Page
117
End Page
122
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT