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ID 32207
JaLCDOI
FullText URL
Author
Okada, Soji
Ichiki, Ken
Sato, Kimiaki
Tanakuchi, So
Ishii, Keita
Hamada, Hiroshi
Ota, Zensuke
Abstract

We studied the pathways of complement activation associated with the islet cell surface antibody (ICSA) obtained from sera of 7 patients (age less than 15 years) with insulin dependent diabetes mellitus (IDDM). The target cells were 51CR labelled rat islet cells and the complement source was human AB serum. Complement-dependent antibody mediated cytotoxicity (CAMC activity) was obtained using the percentage of cytotoxicity. CAMC activity of untreated sera was significantly inhibited by treating with EGTA or EDTA (p less than 0.001). The CAMC activity of EDTA-treated sera was significantly lower than that of EGTA-treated sera (p less than 0.001). In the inactivated human AB serum, it was lower than that of EGTA-treated sera (p less than 0.05), but not different from that of EDTA-treated sera. These results show that the complement activation associated with ICSA in patients occurred not only via the classical pathway but also via the alternative pathway.

Keywords
complement activation
islet surface antibody
insulin-dependent diabetes mellitus
complement-dependent antibody medeated cytotoxicity
classical pathway
alternative pathway
Amo Type
Article
Published Date
1991-06
Publication Title
Acta Medica Okayama
Volume
volume45
Issue
issue3
Publisher
Okayama University Medical School
Start Page
185
End Page
186
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT