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ID 31323
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Author
Wakimoto, Masahiro
Masuoka, Noriyoshi
Nakano, Taku
Ubuka, Toshihiko
Abstract

A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.

Keywords
glutathione peroxidase
erythrocyte
hydrogen peroxide
acatalasemic mouse
Takahara disease
Amo Type
Article
Published Date
1998-10
Publication Title
Acta Medica Okayama
Volume
volume52
Issue
issue5
Publisher
Okayama University Medical School
Start Page
233
End Page
237
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT