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ID 56674
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Senoh, Mitsutoshi Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Ghosh-Banerjee, Jayeeta National Institute of Cholera and Enteric Diseases,
Mizuno, Tamaki Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Shinoda, Sumio Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Miyoshi, Shin-ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Hamabata, Takashi Research Institute, National Center for Global Health and Medicine
Nair, G. Balakrish Translational Health Science and Technology Institute
Takeda, Yoshifumi Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Abstract
Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at <60°C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F-TCBS plates). After confirming that VBNC V. cholerae O1 and O139 formed typical yellow colonies on F-TCBS plates, we tried to isolate cholera toxin gene-positive VBNC V. cholerae from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating V. cholerae O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC V. cholerae O1 as a source of cholera outbreaks is discussed.
Keywords
Factor converting VBNC into culturable
VBNC Vibrio cholerae
Published Date
2014-02-18
Publication Title
Microbiologyopen
Volume
volume3
Issue
issue2
Publisher
Wiley-Blackwell
Start Page
239
End Page
246
ISSN
20458827
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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DOI
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Related Url
isVersionOf https://doi.org/10.1002/mbo3.164
Project
Collaborative Research of Okayama University for Infectious Diseases in India