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ID 16461
Eprint ID
16461
FullText URL
Thumnail 96_665.pdf 606 KB
Title Alternative
Establishment of a T cell hybridoma continuously producing Interleukin 2 (IL2) and functional analysis of secreted IL2
Author
Kanatani, Tomohisa
Abstract
The AKR-derived T lymphoma cell line BW5147 was fused with a Mycobacterium tuberculosis-primed and boosted BALB/c T cell to produce a T cell hybridoma (A55-24) which continuously produces Interleukin 2 (IL2). A55-24 cells produce IL2 without stimulation by lectin or antigen. The hybridoma culture supernatant did not contain other lymphokine-activities, T cell-replacing factor (TRF), B cell growth factor (BCGF), or macrophage activating factor (MAF). The secreted IL2 possessed the following characteristics: 1) IL2 activity was precipitated at an ammonium sulphate saturation of 50-85% . 2) a molecular weight of 30 Kd 3) an isoelectric point of pH 5.3±0.2 4) IL2 activity was destroyed only partially by pH 2 treatment and heating (56℃, 30min), but eliminated by boiling (100℃, 10min). 5) IL2 activity was absorbed by an IL2-dependent cytotoxic T cell line. 6) Addition of IL1 and this IL2 could not generate any significant CTL responses from PNA(+)-thymocytes, whereas further addition of PPD-CFS obtained from PPD-stimulated Mycobacterium-primed T cells induced noticiable responses.
Keywords
interleukin 2
T cell hybridoma
lymphokine
cytotoxic T lymphocyte
Published Date
1984-08-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume96
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
665
End Page
674
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/96/7-8/96_7-8_665/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma