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ID 48284
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Author
Yamamoto, Dai
Ghosh, Souvik
Kuzuya, Mitsutaka
Wang, Yuan-Hong
Zhou, Xuan
Chawla-Sarkar, Mamta
Paul, Shyamal Kumar
Ishino, Masaho
Kobayashi, Nobumichi
Abstract
Group C rotavirus (GCRV) is distributed worldwide as an enteric pathogen in humans and animals. However, to date, whole-genome sequences are available only for a human strain (Bristol) and a porcine strain (Cowden). To investigate the genetic diversity of human GCRVs, nearly full-length sequences of all 11 RNA segments were determined for human GCRVs detected recently in India (v508), Bangladesh (BS347), China (Wu82 and YNR001) and Japan (OH567 and BK0830) and analysed phylogenetically with sequence data for GCRVs published previously. All the RNA segments of human GCRV strains except for the VP3 gene showed high levels of conservation (>93 % nucleotide sequence identity, >92 % amino acid sequence identity), belonging to a single genetic cluster distinct from those of animal GCRVs. In contrast, the VP3 genes of human GCRVs could be discriminated into two clusters, designated M2 and M3, that were distinguished phylogenetically from those of porcine and bovine GCRVs (clusters M1 and M4, respectively). Between M2 and M3, amino acid sequence identity of the VP3 gene was 84.1–84.7 %, whereas high identities were observed within each cluster (92.3–97.6 % for M2, 98.2–99.3 % for M3). Sequence divergence among the four VP3 clusters was observed throughout the amino acid sequence except for conserved motifs, including those possibly related to enzyme functions of VP3. The presence of obvious genetic diversity only in the VP3 gene among human GCRVs suggested that either the M2 or M3 VP3 gene of human GCRVs might have been derived through reassortment from an animal GCRV or from an unidentified human GCRV strain belonging to a novel genogroup.
Published Date
2011-02
Publication Title
Journal of General Virology
Volume
volume92
Issue
issue2
Start Page
361
End Page
369
ISSN
0022-1317
NCID
AA00698722
Content Type
Journal Article
Project
Collaborative Research of Okayama University for Infectious Diseases in India
Official Url
http://vir.sgmjournals.org/content/92/2/361
language
英語
Copyright Holders
© 2011 SGM
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Refereed
True
DOI
PubMed ID
Web of Sience KeyUT