JaLCDOI 10.18926/AMO/31541
FullText URL fulltext.pdf
Author Izumi, Masaki|
Abstract <p>Sera from 24 patients with hepatocellular carcinoma (HCC), 30 patients with hepatobiliary diseases other than HCC and 5 normal subjects were analyzed for gamma-glutamyltransferase (GGT) isozymes. In ultracentrifugation, GGT I' was recovered in the non-lipoprotein fraction (the residue), together with GGTs I'', II', I and X. GGTs III to IX were recovered in lipoprotein fractions. GGTs in the lipoprotein fractions were removed beforehand by Affi-Gel Blue chromatography, leaving GGTs I', I'', II', I and X in the non-bound fraction, which was subjected to Con A-Sepharose chromatography. From the double affinity chromatography (DAC), GGTs I' and II' were recovered in the unbound fraction, and GGTs I, I'', II' and X in the bound fraction. GGT activities in the unbound fractions of sera from HCC patients were generally higher than those from patients with other benign hepatobiliary diseases. When the GGT activity of the unbound fractions in DAC was expressed as a percent of the sum of the unbound and bound activities (U/(U + B)) and 22% was set as the lower limit of positive values, 54% of the HCC cases had positive values, while none of the patients with hepatobiliary diseases other than HCC had positive values. The U/(U + B) ratio of GGT in DAC appears to be a clinically useful test for screening HCC.</p>
Keywords ?-glutamyltransferase hepatocellular carcinoma ultracentrifugation Affi-Gel Blue chromatography Con A-Sepharose chromatography
Amo Type Article
Published Date 1985-02
Publication Title Acta Medica Okayama
Volume volume39
Issue issue1
Publisher Okayama University Medical School
Start Page 19
End Page 33
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2858962
Web of Sience KeyUT A1985ACS5600003
JaLCDOI 10.18926/AMO/31263
FullText URL fulltext.pdf
Author Miyazaki, Masahiro| Matsuura, Kazuhiko| Wahid, Syarifuddin| Izumi, Masaki| Taketa, Kazuhisa| Sato, Jiro|
Abstract <p>Alpha-getoprotein (AFP) was purified from fetal rat serum by a combined technique of affinity chromatography with Affi-Gel Blue and disc electrophoresis followed by extraction of AFP from the gel. The purified AFP was immunologically identical with the original AFP in fetal rat serum.</p>
Keywords purification of rat AFP fetal rat serum affinity chromatography disc electrophoresis.
Amo Type Brief Note
Published Date 1981-12
Publication Title Acta Medica Okayama
Volume volume35
Issue issue6
Publisher Okayama University Medical School
Start Page 427
End Page 430
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6172956
Web of Science KeyUT A1981MV25300007
JaLCDOI 10.18926/AMO/30548
FullText URL fulltext.pdf
Author Ide, Takero| Taketa, Kazuhisa| Watanabe, Makoto| Ikeda, Satoru| Izumi, Masaki| Sokabe, Teruko| Kono, Hiroshi| Yamamoto, Yoshio|
Abstract <p>&lt;p&gt;The levels of HDL-cholesterol and gamma-glutamyltransferase in the sera of 17 patients with alcoholic liver injury were followed after abstinence and compared with those of 11 patients with acute non-alcoholic hepatitis. The activity of gamma-glutamyltransferase decreased in all cases irrespective of the type of liver injuries. The level of HDL-cholesterol also decreased in 11 of 17 cases with alcoholic liver injury. The other alcoholics, in whom HDL-cholesterol level increased or showed no definite change after withdrawal of alcohol, had severe and advanced liver injuries. In non-alcoholic hepatitis, the HDL-cholesterol level increased as normal liver functions were restored except for one case with cholestatic features. It was concluded that alcohol intake can increase HDL-cholesterol level even in the presence of a concomitantly induced hepatic lesion.&lt;/p&gt;</p>
Keywords alcoholic liver injury HDL-cholesterol ?-glutamy1-transferase.
Amo Type Article
Published Date 1980-11
Publication Title Acta Medica Okayama
Volume volume34
Issue issue5
Publisher Okayama University Medical School
Start Page 293
End Page 299
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6108050
Web of Science KeyUT A1980KT96800001