JaLCDOI 10.18926/AMO/32819
FullText URL fulltext.pdf
Author Okamoto, Osamu| Yamamoto, Yuji| Inagaki, Sachiyo| Yoshitome, Kei| ishikawa, Takaki| Imabayashi, Kiyomi| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>Allele and genotype frequencies for 15 short tandem repeat (STR) polymorphisms--D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA--in a Japanese population were estimated. No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for any of the systems studied. Between 2 new pentanucleotide STR loci, Penta E and Penta D, for which there is only limited data regarding the allelic distribution in Japanese, the Penta E locus was found to be highly polymorphic and exhibited a tri- or tetra-modal distribution pattern having allelic peaks with 5, 11, 15 and 20 repeats. The distribution was significantly different from that of the other ethnic groups. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity values (H), polymorphism information content (PIC), power of discrimination (PD), matching probability (pM), power of exclusion (PE), and typical paternity index (PI), were calculated for the loci. These parameters indicated the usefulness of the loci in forensic personal identification and paternity testing among Japanese. The systems Penta E, FGA, D18S51 and D8S1179 were the most informative. This method was successfully applied to forensic personal identification and paternity testing among Japanese, thereby confirming its efficacy for forensic practice.</p>
Keywords population data DNA typing short tandem repests personal identification paternity testing
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 59
End Page 71
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866745
Web of Science KeyUT 000182520400003
JaLCDOI 10.18926/AMO/32818
FullText URL fulltext.pdf
Author Ishikawa, Takaki| Tachibana, Toshiaki| Ishikawa, Hiroshi| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>Healthy subjects 40 years old were used as controls in a study of stellate cells (S-100 protein-containing cells, or S-100 cells) in subjects with chronic alcoholism and fatty liver or fatty cirrhosis. S-100 cells were sparsely found in the adenohypophysis of control subjects, and these cells sometimes formed small clusters. However, in chronic alcoholics with fatty liver or fatty cirrhosis, the number of stellate cells in the anterior pituitary tended to be 17 times higher than it was in the control group. No increase in the number of S-100 positive cells that constitute the large and small follicles in the intermediate pituitary. The physiological function of the S-100 protein has not yet been identified. The fact that an increase in prolactin-secreting and growth hormone-secreting cells, as well as a decrease in gonadotrophs were observed in the hypophysis of alcoholics suggests that the function of stellate cells may be closely related to these phenomena. Our results also imply that the stellate cells found in the anterior and intermediate pituitary differ in function although they both produce S-100 proteins.</p>
Keywords S-100 protein pituitary alcoholism
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 53
End Page 58
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866744
Web of Science KeyUT 000182520400002
JaLCDOI 10.18926/AMO/32816
FullText URL fulltext.pdf
Author Ishikawa, Takaki| Miyaishi, Satoru| Tachibana, Toshiaki| Yamamoto, Yuji| Ishizu, Hideo|
Abstract <p>In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicles also tended to enlarge with age. Statistical analysis showed that a high correlation existed between age and the number or the size of the mixed cell-follicles formed by various adenohypophyseal cells. In addition, when the proportions of the different cell types that formed the follicles were examined, sex differences were observed with aging for the GH cells, the PRL cells, and the gonadotroph (GTH) cells, while no changes were observed with aging in both men and women for the ACTH cells and TSH cells. These results indicate that the number, size, and ratio of each component cell of follicles in the anterior pituitary are adequately applicable for the purpose of age estimation in routine forensic medicine.</p>
Keywords mixed cell-follicle human anterior pituitary age estimation
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 83
End Page 89
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866748
Web of Science KeyUT 000182520400006
JaLCDOI 10.18926/AMO/32509
FullText URL fulltext.pdf
Author Satoh, Kohichi| Ishizu, Hideo| Habara, Toshio| Akiyama, Nobuo| Ueno, Seishi| Kiyotani, Taro| Kondo, Masaru| Yano, Mikio|
Abstract <p>In the present experiments attempts were made to identify semen from various specimens such as the semen itself, spots of semen on clothes, putrefied semen or semen contaminated with blood, menstrual blood, vaginal fluid, according to the techniques of LEVONEN. As the result it has been clarified that in every instance it is possible to isolate and detect the spots of choline by spraying Dragehdorff's reagent.</p>
Amo Type Article
Published Date 1967-02
Publication Title Acta Medicinae Okayama
Volume volume21
Issue issue1
Publisher Okayama University Medical School
Start Page 9 9
End Page 14 14
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4229055
NAID 120002311373
JaLCDOI 10.18926/AMO/32309
FullText URL fulltext.pdf
Author Murakami, Hiroki| Ymamamoto, Yuji| Yoshitome, Kei| Ono, Toshiaki| Okamoto, Osamu| Shigeta, Yoshiaki| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>In this study, sex determination using polymerase chain reaction (PCR) on tooth material was evaluated from the viewpoint of forensic medicine. The sensitivity of PCR for detection of the Y chromosome-specific alphoid repeat sequence and the X chromosome-specific alphoid repeat sequence was 0.5 pg of genomic DNA. Sex could be determined by PCR of DNA extracted from the pulp of 16 freshly extracted permanent teeth and dentine including the surface of the pulp cavity of 6 freshly extracted milk teeth. Sex could be determined using the pulp in all 20 teeth (10 male and 10 female) preserved at room temperature for 22 years. For the pulp of teeth stored in sea water, the sex could be determined in all 8 teeth immersed for 1 week and in 5 of 6 teeth immersed for 4 weeks. In the remaining 1 tooth, in which sex determination based on the pulp failed, the sex could be determined correctly when DNA extracted from the tooth hard tissue was examined. For teeth stored in soil, the sex could be determined accurately in all 8 teeth buried for 1 week, 7 of 8 teeth buried for 4 weeks, and in all 6 teeth buried for 8 weeks. When teeth were heated for 30 min, sex determination from the pulp was possible in all teeth heated to 100, 150, and 200 degrees C, and even in some teeth heated to 250 degrees C. When this method was applied to actual forensic cases, the sex of a mummified body estimated to have been discovered half a year to 1 year after death could be determined readily by examination of the dental pulp. In the skeletons of 2 bodies placed under water for approximately 1 year and approximately 11 years and 7 months, pulp tissues had been dissolved and lost, but sex determination was possible using DNA extracted from hard dental tissues. These results indicate that this method is useful in forensic practices for sex determination based on teeth samples.</p>
Keywords personal identification sex determination tooth deoxyribonucleic acid (DNA). polymerase chain reaction
Amo Type Article
Published Date 2000-02
Publication Title Acta Medica Okayama
Volume volume54
Issue issue1
Publisher Okayama University Medical School
Start Page 21
End Page 32
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10709619
Web of Science KeyUT 000085526000004
JaLCDOI 10.18926/AMO/32024
FullText URL fulltext.pdf
Author Ono, Toshiaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Ishikawa, Takaki| Ishizu, Hideo|
Abstract <p>We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.&#60;/P&#62;</p>
Keywords species identification myoglobin polymerase chain reaction
Amo Type Article
Published Date 2001-06
Publication Title Acta Medica Okayama
Volume volume55
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 184
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11434430
Web of Science KeyUT 000169512600004
JaLCDOI 10.18926/AMO/31971
FullText URL fulltext.pdf
Author Imabayashi, Kiyomi| Yamamoto, Yuji| Inagaki, Sachiyo| Doi, Yusuke| Yoshitome, Kei| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.</p>
Keywords HLA-DRB1 genotyping group specific primer single nucleotide polymorphism multiplex primer extension reactions application to mixed samples
Amo Type Article
Published Date 2005-10
Publication Title Acta Medica Okayama
Volume volume59
Issue issue5
Publisher Okayama University Medical School
Start Page 179
End Page 194
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16286957
Web of Sience KeyUT 000232835600002
JaLCDOI 10.18926/AMO/31726
FullText URL fulltext.pdf
Author Yoshitome, Kei| Ishikawa, Takaki| Inagaki, Sachiyo| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.</p>
Keywords asphyxia suffocation helium advertising balloon atmosphere lacking in oxygen
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 55
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873946
Web of Science KeyUT 000174031300010
JaLCDOI 10.18926/AMO/31707
FullText URL fulltext.pdf
Author Shigeta, Yoshiaki| Yamamoto, Yuji| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104-156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evaluated using various forensic samples. The typing results from 70 DNA samples using the new primer pair and the original primer pair were completely identical. One hundred twenty-five amplified fragments from D12S391 alleles were sized correctly within +/- 0.25 bp of the D12S391 allelic ladder. A rare allele, 19.3, previously found only in Caucasians, was found for the first time in a Japanese subject, and it was clearly distinguished from allele 20 by the CE analysis. This detection system was sensitive and could detect D12S391 types from 16 pg of genomic DNA, and from a minor component at a ratio of 1:10 in mixed samples. This system was more useful for the analysis of degraded DNA than was the method using the original primer pair, and could detect D12S391 types from bloodstains that had been stored for 26 years. In addition, the specificity of the method was demonstrated using nonhuman DNA.</p>
Keywords short tandem repeats D12S391 forensic application capillary electrophoresis
Amo Type Article
Published Date 2002-10
Publication Title Acta Medica Okayama
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 229
End Page 236
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12530506
Web of Science KeyUT 000178668100003
JaLCDOI 10.18926/AMO/31336
FullText URL fulltext.pdf
Author Ito, Akira| Moriya, Fumio| Ishizu, Hideo|
Abstract <p>To establish a method for estimating the time between the last consumption of alcohol and death, we examined the ethanol levels in body fluids and tissues of rats that had been orally administered 1 g/kg ethanol. We observed the following relationships between ethanol levels in the cardiac blood (blood in the heart itself), vitreous humor, and urine: cardiac blood &#62; vitreous humor &#62; urine at 10 min (early absorption stage); vitreous humor &#62; cardiac blood &#62; urine from 20 to 50 min (late absorption stage); vitreous humor &#62; urine &#62; cardiac blood from 60 to 120 min (distribution stage); and urine &#62; vitreous humor &#62; cardiac blood at 180 min (excretion stage). It was also observed that, in cases of death immediately following drinking, ethanol levels in the stomach contents are very high, and the following ratios of ethanol levels were observed: skeletal muscle to cardiac blood--less than 1; liver to cardiac blood--around 1. buccal mucosa to cardiac blood-greater than 1. These ratios at equilibrium after drinking were around 1, lower than 1 and around 1, respectively. We also measured alcohol levels in the cardiac blood, urine, vitreous humor and stomach contents of nine cadavers who had consumed alcohol prior to death. The relationships between the time since last consumption of alcohol and relative ethanol levels in these specimens were in good accordance with the results of the animal experiments. </p>
Keywords toxicology ethyl alcohol ethanol in cadavers tissue distribution of ethanol time between drinking and death
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 8
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548988
Web of Sience KeyUT 000072264100001
JaLCDOI 10.18926/AMO/31334
FullText URL fulltext.pdf
Author Kan, Shin| Moriya, Fumio| Ishizu, Hideo|
Abstract <p>The main purpose of this study was to evaluate the inhibitory effects of 5-fluorouracil antineoplastics, cephem antibiotics containing the methyltetrazolylthiol (MTT) group and antidiabetics on aldehyde dehydrogenase (ALDH) activity in vivo and in vitro. In in vivo experiments, rats were given a 100 mg/kg dose of drugs (10 mg/kg for glibenclamide) orally or intraperitoneally. When each drug was administered singly immediately after an oral administration of 1.5 g/kg ethanol, only carmofur, an antineoplastic, produced marked increases in blood acetaldehyde concentrations. This action was also noted when ethanol was ingested 15 h after administration. The remaining drugs did not increase blood acetaldehyde concentrations. When rats were treated with carmofur at 12 h intervals for 3 consecutive days and were given 1.5 g/kg ethanol after the final treatment, blood acetaldehyde concentrations were elevated more significantly than with a single administration of carmofur. Furthermore, daily administration of cephem antibiotics containing the MTT group, latamoxef, cefamandole, cefoperazone and cefbuperazone, significantly increased blood acetaldehyde concentrations. Daily administration of sulfonylurea antidiabetics, chlorpropamide and acetohexamide, slightly increased blood acetaldehyde concentrations. Drugs causing increases in blood acetaldehyde concentrations when administration was combined with ethanol ingestion also inhibited ALDH activity in vitro. The results of the in vitro experiments roughly correlated with those of the in vivo experiments. The inhibitory effects of drugs on ALDH activity were in the following order: carmofur &#62;&#62; cephem antibiotics containing the MTT group &#62; sulfonylurea antidiabetics.</p>
Keywords toxicology acetaldehyde aldehyde dehydrogenase disulfiram-like reaction carmofur
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 9
End Page 17
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548989
Web of Science KeyUT 000072264100002
JaLCDOI 10.18926/AMO/31301
FullText URL fulltext.pdf
Author Yano, Akemi| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.</p>
Keywords DNA polymorphism haptoglobin polymerase chain reaction allele-specific amplification personal identification
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 173
End Page 181
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781267
Web of Science KeyUT 000075623600001
JaLCDOI 10.18926/AMO/30747
FullText URL fulltext.pdf
Author Redsch, Oliver| Miyaishi, Satoru| Heinemann, Axel| Fiedler, Georg| Puschel, Klaus| Yamamoto, Hideki| Ishizu, Hideo|
Abstract The authors designed a questionnaire to investigate the differences in German and Japanese general practitioners? (GP) awareness of suicide and attitudes toward patients with suicidal ideation in their respective societies. The purpose of this study was to obtain insights leading to a better means of suicide prevention in primary care in Japan. The background for conducting the study was declining suicides in the past 20 years and the lower suicide rate in Germany compared with the present situation in Japan, where the number of suicides has in recent years continued to exceed 30,000, resulting in a suicide rate approximately 2 times higher than that in Germany. The questionnaire was randomly mailed to GPs in Okayama-Prefecture (western Japan) and Hamburg-State (northern Germany) and was collected in the same way. The patterns of answers were compared between the 2 countries, and the differences were statistically analyzed. Japanese GPs seem to have a lower will to prevent suicide in daily practice compared to German GPs and a great lack of knowledge about treatment of suicidal patients. These observations suggest that improving GPs? interest in the problem of suicide and providing training programs for the treatment of patients with suicidal intentions might be a means of achieving better suicide prevention in Japan.
Keywords suicide prevention general practitioner Japan Germany
Amo Type Article
Published Date 2006-06
Publication Title Acta Medica Okayama
Volume volume60
Issue issue3
Publisher Okayama University Medical School
Start Page 159
End Page 165
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16838044
Web of Science KeyUT 000238503600003
JaLCDOI 10.18926/AMO/30512
FullText URL fulltext.pdf
Author Takata, Shingo| Yamamoto, Yuji| Ishizu, Hideo|
Abstract <p>A method of genotyping IgA2 alleles in the human immunoglobulin alpha 2 heavy chain constant region (C alpha 2 gene) was developed by using the polymerase chain reaction (PCR). By this method, the genotype was determined by discriminating base substitution in the 3'-flanking region of alleles, A2m*1 and A2m*2, which manifest A2m serum types, by nested PCR using allele-specific primers. Three types, IgA2*1/IgA2*1, IgA2*2/IgA2*1, and IgA2*2/IgA2*2, were detected from DNA extracted from lymphocytes. Genotyping was possible from 100 pg of DNA by this method. The estimated allele frequency in 318 Japanese subjects was 0.561 for IgA2*1 and 0.439 for IgA2*2. Analysis of 29 cases of paternity tests suggested that the data follow Mendel's law of inheritance. This genotype could also be detected in whole blood, blood stains, saliva stains, and various organs and tissues. These results suggest the usefulness of the present method for paternity testing and individual identification in forensic medicine.</p>
Keywords polymorphism deoxryibonucleic acid(DNA) immunoglobulin alpha 2 polymerase chain reaction(PCR) allele-specific amplificartion
Amo Type Article
Published Date 1996-02
Publication Title Acta Medica Okayama
Volume volume50
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 9
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8701775
Web of Science KeyUT A1996TY06000001