JaLCDOI 10.18926/AMO/31753
FullText URL fulltext.pdf
Author Ogata, Masana| Fujii, Yasuhito| Meguro, Tadamichi| Kira, Shohei| Matsuda, Akira| Izushi, Fumio| Kimoto, Tetsuo| Takahara, Shigeo|
Abstract In an attempt to determine the level and heat stability of residual catalase in somatic cells of acatalasemic Japanese, skin fibroblasts from an acatalasemic subject were cultured, and the catalase activity of the cultured fibroblasts was compared with that of cultured normal fibroblasts. Catalase activity was determined using an oxygen electrode. The residual catalase activity in cultured acatalasemic fibroblasts was 10% of the normal. The heat stability at 55 degrees C of residual catalase in the acatalasemic fibroblasts was similar to that of normal fibroblasts.
Keywords tissue culture skin fibroblast actalasemia catalase
Amo Type Article
Published Date 1987-10
Publication Title Acta Medica Okayama
Volume volume41
Issue issue5
Publisher Okayama University Medical School
Start Page 201
End Page 204
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders Copyright© 1999 Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 3687491
Web of Science KeyUT A1987K590100003
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/5561
JaLCDOI 10.18926/AMO/31035
FullText URL fulltext.pdf
Author Izushi, Fumio| Mori, Takaaki| Ogata, Masana|
Abstract <p>The in vitro effects of phenol and p-halogenated phenols on mitochondrial energy transfer reactions were examined using isolated rat liver mitochondria. The relationship between physiochemical properties of phenolic compounds and their effects on mitochondria were studied. Phenol and p-halogenated phenols induced the release of K+ ions from mitochondria, suggesting a change in permeability to K+ ions. A decrease in the respiratory control index, an increase in K+ release and stimulation of latent ATPase activity were observed with these compounds in the descending order of p-iodophenol, p-bromophenol, p-chlorophenol, p-fluorophenol and phenol. The concentrations of the phenolic compounds resulting in fifty percent inhibition of the respiratory control index and those resulting in fifty percent release of K+ ions significantly correlated with Hammett's substituent constant (sigma) and the hydrophobic binding constant (pi) of the compounds.</p>
Keywords phenol mitochondria oxidative phosphorylation Hammett's substituent constant hydrophobic bindung constant
Amo Type Article
Published Date 1988-02
Publication Title Acta Medica Okayama
Volume volume42
Issue issue1
Publisher Okayama University Medical School
Start Page 7
End Page 14
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2966543
Web of Science KeyUT A1988M237800002