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ID 33011
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Author
Chuanuwatanakul, Suchada
Chailapakul, Orawon
Motomizu, Shoji
Abstract

The electrochemical properties of chloramphenicol at a boron-doped diamond thin-film (BDD) electrode were studied using cyclic voltammetry. The highest current response of chloramphenicol was obtained with phosphate buffer, pH 6 (0.1 M) in 1% ethanol. The relationship between the concentration of chloramphenicol and the current response was linear over the range of 0.1 - 10 mM (R-2 = 0.9990). The amount of chloramphenicol was analyzed by flow-injection analysis. A thin-layer flow cell equipped with a BDD electrode was used as an amperometric detector, and experiments were carried out at -0.7 V (vs. Ag/AgCl). The linear relationship between the current response and the concentration of chloramphenicol in the range of 0.1 - 50 mu M (R-2 = 0.9948) and the limit of detection of 0.03 mu M (S/N = 3) were obtained. This method has been successfully applied to the determination of chloramphenicol in sterile eye drops and milk sample by the standard addition method. The average recoveries of chloramphenicol in eye drops were 98.0%, and the average recoveries of chloramphenicol from spiked milk were 93.9 - 103%.

Keywords
THIN-FILM ELECTRODE
TANDEM MASS-SPECTROMETRY
CAPILLARY-ZONE-ELECTROPHORESIS
LIQUID-CHROMATOGRAPHY
AMPEROMETRIC DETECTION
PHOTOCHEMICAL-REACTION
OXIDATION
PRODUCTS
RESIDUES
CARBON
Note
Published with permission from the copyright holder.
This is published in Analytical Sciences, 2008 Vol.24 Issue.4 pp.493-498
Publisher URL: http://dx.doi.org/10.2116/analsci.24.493
Direct access to Thomson Web of Science record
(C)2008 by The Japan Society for Analytical Chemistry
Published Date
2008-04-10
Publication Title
Analytical Sciences
Volume
volume24
Issue
issue4
Start Page
493
End Page
498
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
analytical_chemistry/25