JaLCDOI 10.18926/AMO/31026
FullText URL fulltext.pdf
Author Senoo, Yoshimasa| Katoh, Kazuo| Nakai, Yumi| Hashimoto, Yutaka| Bando, Ko| Teramoto, Shigeru|
Abstract <p>The stability of recombinant human superoxide dismutase (r-hSOD) in buffer solutions was studied in solutions at various pH and temperatures. Additionally, we studied the effects of incubation with proteases, serum and two types of hypothermic perfusates. R-hSOD was stable in the pH range of 6-11 and at temperatures up to 80 degrees C for 30 min. R-hSOD activity was not affected by incubation with trypsin, aminopeptidase M or serum for 2 h. R-hSOD activity determined at various temperatures (4-37 degrees C) did not vary remarkably. R-hSOD in hypothermic perfusates was stable at 4-37 degrees C for 24 h.</p>
Keywords recombinant human superoxide dismutase activity stability hypothermic perfusate
Amo Type Article
Published Date 1988-06
Publication Title Acta Medica Okayama
Volume volume42
Issue issue3
Publisher Okayama University Medical School
Start Page 169
End Page 174
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3041738
Web of Sience KeyUT A1988P034000007
JaLCDOI 10.18926/AMO/30513
FullText URL fulltext.pdf
Author Takagaki, Masami| Hisamochi, Kunikazu| Morimoto, Toru| Bando, Ko| Sano, Shunji| Shimizu, Nobuyoshi|
Abstract <p>A shortage of donor organs in clinical transplantation prompted us to study whether resuscitated dead hearts could be utilized for successful orthotopic heart transplantation. After 60 min of hypoxic cardiac arrest, one group of canine hearts was resuscitated (Res group, n = 6). The other group was harvested directly (Non-Res group, n = 6). In the Res group, cardiopulmonary bypass was utilized for resuscitation at 37 degrees C and the animals were then core-cooled to 15 degrees C. The hearts then were preserved in University of Wisconsin solution and orthotopically transplanted. Stable prostacyclin analogue (OP2507) and verapamil, a calcium antagonist, were added to the cardioplegia, and substrate-enriched warm blood cardioplegia and a hydroxy radical scavenger (EPC) were administered at the time of reperfusion of the transplanted heart. All animals in each group were successfully weaned from cardiopulmonary bypass with dopamine (5 micrograms/kg/min). Cardiac function without dopamine was better preserved in the Res group than the Non-Res group (Emax: 130.6 +/- 41.5% vs. 47.1 +/- 24.7%; mean +/- SD, as percent of postbrain death values, P &#60; 0.01 by unpaired t-test). Cadaver hearts 60 min after anoxic arrest can be successfully re-animated and orthotopically engrafted. In addition, the core-cooling technique is useful. We believe this study serves as the key step in the clinical application of dead hearts to successful cardiac transplantation.</p>
Keywords heart transplantation cadaver heart corecooling Emax
Amo Type Article
Published Date 1996-02
Publication Title Acta Medica Okayama
Volume volume50
Issue issue1
Publisher Okayama University Medical School
Start Page 17
End Page 24
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8701777
Web of Science KeyUT A1996TY06000003