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ID 53012
FullText URL
Author
Alamusi
Matsuo, Toshihiko ORCID Kaken ID researchmap
Tsutsui, Kimiko M.
Uchida, Tetsuya Kaken ID publons
Abstract
We have developed a photoelectric dye-coupled polyethylene film as a prototype of retinal prosthesis, which we named Okayama University-type retinal prosthesis. The purposes of this study are to conduct behavior tests to assess vision in Royal College of Surgeons (RCS) rats that underwent subretinal implantation of the dye-coupled film and to reveal retinal response to the dye-coupled film by immunohistochemistry. Polyethylene films were made of polyethylene powder at refined purity, and photoelectric dyes were coupled to the film surface at higher density compared with the prototype. Either dye-coupled film or dye-uncoupled plain film used as a control was implanted subretinally from a scleral incision in both eyes of an RCS rat at 6 weeks of the age. Behavior tests 2, 4, 6, and 8 weeks after implantation were conducted by observing head turning or body turning in the direction consistent with clockwise or counterclockwise rotation of a black-and-white-striped drum around a transparent cage housed with the rat. After the behavior tests at 8 weeks, rats' eyes were enucleated to confirm subretinal implantation of the films and processed for immunohistochemistry. In the behavior tests, the number of head turnings consistent with the direction of the drum rotation was significantly larger in RCS rats with dye-coupled- compared with plain-film implantation [P < 0.05, repeated-measure analysis of variance (ANOVA), n = 7]. The number of apoptotic neurons was significantly smaller in eyes with dye-coupled- compared with plain-film implantation (P < 0.05, Mann-Whitney U test, n = 6). In conclusion, subretinal implantation of photoelectric dye-coupled films restored vision in RCS rats and prevented the remaining retinal neurons from apoptosis.
Keywords
Retinal prosthesis
Photoelectric dye
Polyethylene film
RCS rat
Behavior test
Apoptosis
Immunohistochemistry
Published Date
2013-09
Publication Title
Journal of Artificial Organs
Volume
volume16
Issue
issue3
Start Page
343
End Page
351
ISSN
1434-7229
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52968
language
英語
File Version
author
Refereed
True
DOI
Web of Science KeyUT