Author Fang, Xing| Nunoshiba, Tatsuo| Yoshida, Midori| Nishikawa, Akiyoshi| Nemoto, Kiyomitsu| Degawa, Masakuni| Arimoto, Sakae| Okamoto, Keinosuke| Takahashi, Eizo| Negishi, Tomoe|
Published Date 2014-02-28
Publication Title Genes and Environment
Volume volume36
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/30947
FullText URL fulltext.pdf
Author Khan, Rasel| Takahashi, Eizo| Nakura, Hironori| Ansaruzzaman, Mohammad| Banik, Sukalyani| Ramamurthy, Thandavarayan| Okamoto, Keinosuke|
Abstract <p>Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria.</p>
Keywords Aeromonas water toxin salinity
Amo Type Original Article
Published Date 2008-12
Publication Title Acta Medica Okayama
Volume volume62
Issue issue6
Publisher Okayama University Medical School
Start Page 363
End Page 371
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
Web of Sience KeyUT 000262025000002
Author Takahashi, Eizo|
Published Date 2005-03-25
Publication Title
Content Type Thesis or Dissertation