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ID 47723
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Author
Maekawa, Tetsuya
Abstract
Insulin-like growth factor 1 (IGF1) Is Involved in the proliferation of mouse and rat endometrial cells in a paracrine or autocrine manner. Insulin-like growth factor binding protein-3 (IGFBP3) modulates actions of IGFs directly or indirectly. The present study aimed to determine whether IGFBP3 is Involved In the regulation of proliferation of mouse endometrial cells. Mouse endometrial epithelial cells and stromal cells were isolated, and cultured In a serum free medium. IGF1 stimulated DNA synthesis by endometrial epithelial and stromal cells, and IGFBP3 Inhibited IGF1-induced DNA synthesis. Estradiol-17 beta (E2) decreased the Igfbp3 mRNA level in endometrial stromal cells, whereas It Increased the Igf1 mRNA level. Transforming growth factor alpha (TGF alpha) significantly decreased IGFBP3 expression at both the mRNA and secreted protein levels in endometrial stromal cells. Progesterone (134) did not affect the E2-induced down-regulation of Igfbp3 mRNA expression in endometrial stromal cells, although P4 alone increased Igfbp3 mRNA levels. The present findings suggest that in mouse endometrial stromal cells E2 enhances IGF1 action through enhancement of IGF1 synthesis and reduction of IGFBP3 synthesis, and that TGF alpha affects IGF1 actions through modulation of IGFBP3 levels.
Keywords
IGFBP3
IGF1
estrogen
mouse
uterus
Published Date
2009-02
Publication Title
Zoological Science
Volume
volume26
Issue
issue2
Publisher
Zoological Society of Japan
Publisher Alternative
日本動物学会
Start Page
131
End Page
138
ISSN
0289-0003
NCID
AA10545874
Content Type
Journal Article
language
英語
Copyright Holders
© 2009 Zoological Society of Japan
File Version
publisher
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT