FullText URL ijo_49_3_877.pdf
Author Yamada, Hiroshi| Takeda, Tetsuya| Michiue, Hiroyuki| Abe, Tadashi| Takei, Kohji|
Abstract The endocytic protein dynamin participates in the formation of actin-based membrane protrusions such as podosomes, pseudopodia, and invadopodia, which facilitate cancer cell migration, invasion, and metastasis. However, the role of dynamin in the formation of actin-based membrane protrusions at the leading edge of cancer cells is unclear. In this study, we demonstrate that the ubiquitously expressed dynamin 2 isoform facilitates cell migration by stabilizing F-actin bundles in filopodia of the lung cancer cell line H1299. Pharmacological inhibition of dynamin 2 decreased cell migration and filopodial formation. Furthermore, dynamin 2 and cortactin mostly colocalized along F-actin bundles in filopodia of serum-stimulated H1299 cells by immunofluorescent and immunoelectron microscopy. Knockdown of dynamin 2 or cortactin inhibited the formation of filopodia in serum-stimulated H1299 cells, concomitant with a loss of F-actin bundles. Expression of wild-type cortactin rescued the punctate-like localization of dynamin 2 and filopodial formation. The incubation of dynamin 2 and cortactin with F-actin induced the formation of long and thick actin bundles, with these proteins colocalizing at F-actin bundles. A depolymerization assay revealed that dynamin 2 and cortactin increased the stability of F-actin bundles. These results indicate that dynamin 2 and cortactin participate in cell migration by stabilizing F-actin bundles in filopodia. Taken together, these findings suggest that dynamin might be a possible molecular target for anticancer therapy.
Published Date 2016-06-30
Publication Title International Journal of Oncology
Volume volume49
Issue issue3
Publisher Spandidos
Start Page 877
End Page 886
ISSN 1019-6439
NCID AA10992511
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version publisher
PubMed ID 27572123
DOI 10.3892/ijo.2016.3592
Web of Sience KeyUT 000382447300003
Related Url isVersionOf https://doi.org/10.3892/ijo.2016.3592
Author Michiue, Hiroyuki|
Published Date 2015-09
Publication Title Okayama University Medical Research Updates
Volume volume14
Content Type Others
Author Onishi, Manabu| Kurozumi, Kazuhiko| Ichikawa, Tomotsugu| Michiue, Hiroyuki| Fujii, Kentaro| Ishida, Joji| Shimazu, Yosuke| Chiocca, E Antonio| Kaur, Balveen| Date, Isao|
Published Date 2013-04-15
Publication Title SpringerPlus
Volume volume2
Content Type Journal Article
Sort Key 1
Title Alternative The 2013 Incentive Award of the Okayama Medical Association in General Medical Science (2013 Yuuki Prize)
FullText URL 127_1.pdf
Author Michiue, Hiroyuki|
Note 平成25年度岡山医学会賞紹介記事 (The 2013 Okayama Medical Association Award)
Publication Title 岡山医学会雑誌
Published Date 2015-04-01
Volume volume127
Issue issue1
Publisher 岡山医学会
Publisher Alternative Okayama Medical Association
Start Page 1
End Page 3
ISSN 0030-1558
NCID AN00032489
Content Type Journal Article
language 日本語
Copyright Holders Copyright (c) 2015 岡山医学会
File Version publisher
Refereed True
DOI 10.4044/joma.127.1
NAID 130005068346
Eprints Journal Name joma
Author Masumoto, Toshio| Suzuki, Koichiro| Ohmori, Iori| Michiue, Hiroyuki| Tomizawa, Kazuhito| Fujimura, Atsushi| Nishiki, Tei-ichi| Matsui, Hideki|
Published Date 2012-01
Publication Title Molecular and Cellular Neuroscience
Volume volume49
Issue issue1
Content Type Journal Article
Author Onishi, Manabu| Ichikawa, Tomotsugu| Kurozumi, Kazuhiko| Fujii, Kentaro| Yoshida, Koichi| Inoue, Satoshi| Michiue, Hiroyuki| Chiocca, E. Antonio| Kaur, Balveen| Date, Isao|
Published Date 2013-04
Publication Title Neuropathology
Volume volume33
Issue issue2
Content Type Journal Article
Author Fujimura, Atsushi| Michiue, Hiroyuki| Nishiki, Tei-ichi| Ohmori, Iori| Wei, Fanyan| Matsui, Hideki| Tomizawa, Kazuhito|
Published Date 2011-05-14
Publication Title PLoS ONE
Volume volume6
Issue issue3
Content Type Journal Article
Author Candan, Gerile| Michiue, Hiroyuki| Ishikawa, Sanae| Fujimura, Atsushi| Hayashi, Keiichiro| Uneda, Atsuhito| Mori, Akiko| Ohmori, Iori| Nishiki, Tei-ichi| Matsui, Hideki| Tomizawa, Kazuhito|
Published Date 2012-09
Publication Title Biomaterials
Volume volume33
Issue issue27
Content Type Journal Article
Author 道上 宏之| 富澤 一仁| 魏 范研| 松下 正之| 陸 雲飛| 市川 智継| 田宮 隆| 松井 秀樹| 伊達 勲|
Published Date 2007-01-04
Publication Title 岡山医学会雑誌
Volume volume118
Issue issue3
Content Type Journal Article
Author Michiue, Hiroyuki|
Published Date 2005-06-30
Publication Title
Content Type Thesis or Dissertation