Author Kuroda, Masahiro| Asaumi, Junichi| Nishikawa, Koji| Tanaka, Seiryo| Gao, Xian Shu| Yamamoto, Michinori| Makihata, Eiichi| Hiraki, Yoshio| Kawasaki, Shoji|
Published Date 1993-02
Publication Title 岡山医学会雑誌
Volume volume105
Issue issue1-2
Content Type Journal Article
JaLCDOI 10.18926/11843
Title Alternative Relationship between intracellular uptake of adriamycin and membrane potential in ADR resistant Ehrlich ascites tumor cells
FullText URL 003_081_085.pdf
Author Asaumi, Jun-ichi| Kawasaki, Shoji| Nishikawa, Koji| Shu Gao, Xian| Kuroda, Masahiro| Hiraki, Yoshio|
Abstract We observed adiamycin (ADR) uptake and cellular transmembrane potential [amount of intracellular fluorescence of 3,3'- (Di-n-hexyl)- 2,2'- oxacarbocyanine iodide (NK-2280)] in ADR-resistant cells established from Ehrlich ascites tumor cells (EATC) and wild type EATC. In ADR-resistant cells, ADR uptake and the cellular transmembrane potential decreased as the degree of resistance increased. 4,4'- diisothiocyanatostilbene- 2,2'- disulfonic acid (DIDS) induced markedly decreases of ADR uptake and the cellular transmembrane potential. A good correlation was observed between ADR uptake and transmembrane potential in cultured cells.
Keywords Adriamycin Cell Membrane Potential Flow Cytometry ADR-Resistant Cells DIDS
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 81
End Page 85
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313508