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ID 15911
Eprint ID
15911
FullText URL
Thumnail 100_371.pdf 5.77 MB
Title Alternative
Maintenance and growth-stimulation of primary cultured endothelial cells of the rat liver sinusoid by fresh serum from the portal vein
Author
Araki, Yasuyuki
Abstract
Sinusoidal endothelial cells were isolated from a rat liver by collagenase perfusion and centrifugal elutriation. The cells attached poorly to a plastic dish without coating it even after 24 hr of culture. In the collagen-coated dish, a large number of cells attached to the dish and spread within 24 hr of culture. Centrifugation of the dish immediately after seeding lead to much better attachment of the cells. Fresh rat serum obtained from the portal vein did not increase the attachment, but was essential for the maintenance of the attached cells after the initial 24 hr of culture, when the fetal calf serum was not replaced. The rat serum also induced cell proliferation as determined by thymidine uptake. Its effect was abolished by heattreatment of 56℃ for 30 minutes.
Keywords
ラット血清
肝類洞内皮細胞
初代培養
増殖誘導
Published Date
1988
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume100
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
371
End Page
377
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/100/3-4/100_3-4_371/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma