FullText URL JRNC_313_1_169.pdf
Author Kudo, Ken-ichi| Hanafusa, Tadashi| Ono, Toshiro|
Keywords Monoterpenes Linalool X-ray irradiation Reactive oxygen species SSB
Note The final publication is available at Springer
Published Date 2017-07
Publication Title Journal of Radioanalytical and Nuclear Chemistry
Volume volume313
Issue issue1
Publisher Springer
Start Page 169
End Page 174
ISSN 0236-5731
NCID AA11120189
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
Related Url https://doi.org/10.1007/s10967-017-5268-0
Author Nishiyama, Yuichi| Hanafusa, Tadashi| Yamashita, Jun| Yamamoto, Yoko| Ono, Toshiro|
Published Date 2015-06-20
Publication Title Journal of Radioanalytical and Nuclear Chemistry
Content Type Journal Article
Author Hanafusa, Tadashi| Mohamed, Ali Eldib Ali| Kitaoka, Kenta| Ohue, Yoshihiro| Nakayama, Eiichi| Ono, Toshiro|
Published Date 2011-02-01
Publication Title Cancer Letters
Volume volume301
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/32656
FullText URL fulltext.pdf
Author Hada, Hajime| Koide, Norio| Hanafusa, Tadashi| Sakaguchi, Kosaku| Shinji, Toshiyuki| Sasaki, Shunsuke| Oka, Takahiko| Takayama, Niro| Yumoto, Yasuhiro| Tsuji, Takao|
Abstract <p>We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of E1 envelope protein and beta-galactosidase as an antigen. The antibody to HCV E1 (anti-HCV E1) was detected in 8 (42%) of 19 patients positive for HCV-RNA (16 were positive and 3 were negative for antibody to C100-3) and in 1 (17%) of 6 patients negative for HCV-RNA but positive for antibody to C100-3. HCV-RNA was detected in 8 (89%) of 9 anti-HCV E1 positive sera. The value of alanine aminotransferase was significantly higher in patients positive for anti-HCV E1 than in patients negative for the antibody. Although an antibody to the envelope protein of HCV is suspected to be one of the candidates of virus-neutralizing antibodies, our results suggest this hypothesis appears to be unlikely.</p>
Keywords hepatitis C virus envelope antibody Western blotting
Amo Type Article
Published Date 1992-10
Publication Title Acta Medica Okayama
Volume volume46
Issue issue5
Publisher Okayama University Medical School
Start Page 367
End Page 370
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1279946
Web of Sience KeyUT A1992JX49500007
JaLCDOI 10.18926/AMO/32196
FullText URL fulltext.pdf
Author Hada, Hajime| Koide, Norio| Takabatake, Hiroyuki| Hanafusa, Tadashi| Tsuji, Takao|
Abstract It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3%) were significantly lower than those within each subtype (83.9-93.5%). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.
Keywords hapatitis C virus envelope DNA sequecing homology intersubtype variable region
Amo Type Article
Published Date 1991-10
Publication Title Acta Medica Okayama
Volume volume45
Issue issue5
Publisher Okayama University Medical School
Start Page 347
End Page 355
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1661558
Web of Sience KeyUT A1991GN53800009
Author Hanafusa, Tadashi| Shinji, Toshiyuki| Shiraha, Hidenori| Nouso, Kazuhiro| Iwasaki, Yoshiaki| Yumoto, Eichiro| Ono, Toshiro| Koide, Norio|
Published Date 2005-01-20
Publication Title BMC Cancer
Volume volume5
Content Type Journal Article
Author Nagamatsu, Tomohiro| Hanafusa, Tadashi| Kinno, Ikuo| Sakoda, Akihiro| Hanamoto, Katsumi| Yamaoka, Kiyonori| Ono, Toshiro|
Published Date 2008-12
Publication Title 環境制御
Volume volume30
Content Type Journal Article
Author 花房 直志|
Published Date 1989-03-28
Publication Title
Content Type Thesis or Dissertation