Author Yabuta, Takeshi|
Published Date 2004-03-25
Publication Title
Content Type Thesis or Dissertation
JaLCDOI 10.18926/15363
FullText URL Mem_Fac_Eng_OU_34_39.pdf
Author Hayakawa, Satoshi| Shirosaki, Yuki| Yabuta, Takeshi| Tsuru, Kanji| Ohtsuki Chikara| Osaka, Akiyoshi|
Abstract Silicone elastomer was chemically treated at 60℃ for 7 days with 30 wt% H(2)O(2) solutions with or without TaCl(5) and soaked for various periods in a simulated body fluid(Kokubo solution) up to 21 days. Apatite formation ability of the surface of the silicone elastomer specimens was investigated with thin-film X-ray diffraction and FT-IR reflection spectroscopy. These silicone specimens did not deposit apatite or calcium phosphates, irrespective of chemical treatment. Osteoblast-like cells (MC3T3-El) derived from mouse were cultured on the specimens at 36.5℃ under 5%C0(2) and 95% humidity. Similar degree of proliferation of cells was observed at 7 days among three specimens, while the no treatment specimen after incubation for 5 days showed a lower degree of proliferation than the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). Alkaline phosphatase activity of the cells proliferated on the no treatment specimen was lower than those of the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). These results indicate that the cytotoxicity of the silicone could be improved by the chemical treatment with 30 wt% H(2)O(2) solutions with or without TaCl(5).
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2000-03-27
Volume volume34
Issue issue1-2
Start Page 39
End Page 43
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002308019