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ID 42575
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Title Alternative
Studies on the Influence of Buffer on the Bacterial Respiratiration
Author
Yabe, Yoshiro
Akita, Yoshimi
Akita, Kazuo
Abstract
The preparation of resting cells is one of the most important and most fundamental procedures for the physiological studies on bacteria. In order to know how we should do to get the resting cells suitable to the purposes of experiments, the author studied the influence of washing and suspending on the respiratory activity of bacteria. The results are summarized as follows: 1) For the purpose to obtain the resting cells of high respiratory activity, washing with phosphate buffer is better than that with distilied water or physiological saline solution. 2) When the prepared resting cells are used in a short time after the preparation, the respiratory activity is the same regardless of the sorts of the suspending solutions, distilled water, saline solution and phosphate buffer. 3) Borate and phthalate buffers of high concentration somewhat inhibit the respiration. However, in order to make the shift of pH less, M/15 (final concentration) is good. Phosphate buffer, even of M/15, does not inhibit the respiration. 4) In borate or phthalate buffers, the fall of respiratory activity is diminished by the addition of small amount of phospate.
Published Date
1957-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume69
Issue
issue6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1527
End Page
1531
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/69/6/69_6_1527/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
Copyright© 岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma