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ID 16213
Eprint ID
16213
FullText URL
Thumnail 96_61.pdf 322 KB
Title Alternative
Studies on Complex-release Activity of Complement in Systemic Lupus Erythematosus -Using Peroxidase as an Antigen in the Immune Complex- Part 1. Studies on Measuring Complex-release Activity
Author
Aibara, Yasushi
Abstract
A simplified method of measuring the complex-release activity (CRA) of complement using peroxidase (PO) as an antigen in the immune complex was studied. PO-anti PO rabbit IgG immune complex (PO-IC) was solubilized principally via the alternative pathway in the sera. When substrates, H(2)O(2) and 5-aminosalicylic acid, were added to solubilized PO-IC, absorbance of the mixture at O.D. 450nm increased with the amount of complement in a dose-dependent manner. The CRA of the complement in the sera was shown by the absorbance of the mixture. The CRA in various sera correlated with CH50. The CRA via the alternative pathway (ACRA) in sera also correlated with CRA, CH50 and ACH50.The above results indicate that this method of measuring the complex-release activity of complement using peroxidase as an antigen is simple and useful.
Keywords
可溶化現象
immune complex
alternative complement pathway
全身性エリテマトーデス
Published Date
1984-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume96
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
61
End Page
67
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/96/1-2/96_1-2_61/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma