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ID 13787
Eprint ID
13787
FullText URL
Thumnail 106_39.pdf 588 KB
Title Alternative
The mechanism of human basophil activation associated with IgG receptor : Analysis of calcium mobilization in the human basophilic
Author
Kawata, Noriko
Abstract
To clarify the mechanism of human basophil activation via IgG receptors, calcium mobiliza-tion in response to IgG antibodies was analyzed in the human basophilic leukemia cell line KU812-F, using flow cytometry. KU812-F cells as well human basophils selectively expres-sed the FcγRⅡ subtype of IgG receptor. After stimulation with aggregated IgG, an obvious increase in [Ca(2+)]i was observed, but the increase was completely inhibited by Ⅳ. 3 (antiFcγRⅡmAb). Moreover, Ⅳ. 3 elicited a[Ca(2+)]i rise only when cross-linked on the cell surface with anti-mouse IgG. No significant histamine release was observed after any IgG stimulation and the biologic function of the FcγRⅡ-induced [Ca(2+)]i rise remains unclar. These findings suggest that the cross-linking of FcγRⅡ in KU812-F cells induces signal transduction events and initiates cell activation with the exception of histamine release, and human basophils also may be activated in vivo by IgG antibodies.
Keywords
KU812-F
Basophil
Ca(2+) response
FcγRⅡ
IgG antibody
Note
原著
Published Date
1994-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
39
End Page
49
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
http://www.journalarchive.jst.go.jp/japanese/jnlabstract_ja.php?cdjournal=joma1947&cdvol=106&noissue=1-2&startpage=39
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma