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ID 11037
Eprint ID
11037
FullText URL
Title Alternative
Photochemical Products from Ergosterol in the Plasma Membrane of the Yeast Rhodotorula minuta cells illuminated by Near-UV
Author
Nakauma, Makoto
Iriyama, Takuya
Tada, Mikiro
Abstract
When the plasma mumbrane of the yeast Rhodotorula minuta cells was exposed to near-UV at 0℃, three new compounds were formes photochemically with decrease in ergosterol content. When 0.4%-SDS solution containing ergosterol was illuminated with near-UV, the same three compounds as in the membrane were formed. As a result of instruments analysis, one of three photochemical products was identified as previtamine D2 and another two compounds were characterized chemically that they had the structure of ergosta-4, 7, 22-trein-3-on. In addition, these photochemical products did not affect the growth and carotenogenesis of the yeast Rhodotorulaminuta.
Abstract Alternative
赤色酵母R.minuta細胞膜に長波長紫外光を照射した時に生じるエルゴステロール由来の光化学反応生成物の単離・精製およびその同定を行った。結果、3種類の物質が単離・精製され、そのうち1つはPrevitamin D2であることが同定され、残りの2つについてはergosta-4,7,22-trien-3-on の何かの炭素にOH基が付いたものであることが分かった。単離した成分を培地に加え、菌体の増殖とカロテノイド生合成に対する影響を調べた結果、これらの物質はそのどちらにも影響を及ぼさなかった。
Keywords
Rhodotorula minuta
near-UV
ergosterol
photochemical products
Published Date
2000-02
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific reports of the Faculty of Agriculture, Okayama University
Volume
volume89
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture,Okayama University
Start Page
45
End Page
51
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa