JaLCDOI 10.18926/11579
Title Alternative 気管支喘息患者末梢血好塩基球の抗ヒトIgEおよびカルシウムイオノフォァA23187に対する反応性について
FullText URL 061_016_022.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Kawauchi, Kazuhisa| Mifune, Takashi| Okuda, Hiroyuki| Komagoe, Haruki| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract The release of histamine from basophils induced by anti-lgE and calcium ionophore A 23187 ( Cal) was examined in 27 patients with bronchial asthma and 7 healthy subjects, using a whole blood method. (1)The release of histamine induced by anti-IgE and CaI was significantly higher in atopic asthmatics than in non-atopic cases. (2) The histamine release with anti-lgE and CaI was significantly lower in cases with long-term steroid therapy compared to the release in cases without steroid therapy. (3) The release with anti-lgE and CaI was increased in cases with age of 0-39 years and in cases wpth age at onset of 0-39 years. (4) Anti-IgE induced release correlated to a certain extent with the release induced by CaI. These results show that basophil reactivity as expressed by histamine release changes under different conditions.
Abstract Alternative 気管支喘息27例および健康人7名を対象に,抗ヒトIgEおよびカルシウムイオノフォァA23187(CaI)刺激時の末梢血好塩基球からのヒスタミン遊離について検討を加えた。(1)抗ヒトIgEおよびCaI刺激時の好塩基球からのヒスタミン遊離は,非アトピー型喘息に比べ,アトピー型喘息において有意に高い値を示した。(2)これらの刺激物質によるヒスタミン遊離は,非使用例に比べ,ステロイド依存性喘息において低い傾向がみられた。また,年齢では39才以下,発症年齢では39才以下でヒズタミン遊離がより高い傾向がみられた。(3)血清IgE値の高い症例の好塩基球は,低い症例に比べより多くのヒスタミンを遊離する傾向がみられた。(4)全般的には,抗ヒトIgE刺激によるヒスタミン遊離とCaI刺激時の遊離との間にはある程度の相関がみられた。以上の結果より,好塩基球の反応性はいろいろの条件下でかなり異なることが示唆された。
Keywords Histamine release (ヒスタミン遊離) Basophils (好塩基球) Anti-lgE (抗ヒトIgE) Calcium ionophore A 23187 (カルシウムイオノフォァA23187) Bronchial asthma (気管支喘息)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 16
End Page 22
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002313673
JaLCDOI 10.18926/11625
Title Alternative Spa therapy for chronic respiratory diseases - in subjects admitted in 1989. -
FullText URL 061_007_015.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Kawauchi, Kazuhisa| Mifune, Takashi| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract 1989年1月より12月までの1年間に当院へ入院した慢性呼吸器疾患患者85例(延べ90例)を対象に,その背景因子,臨床的特徴,および温泉療法の臨床効果について検討を加えた。(1)対象85例のうちわけは,気管支喘息65例,肺気腫4例,肺結核症4例,アレルギー性肉芽腫性血管炎3例,閉塞性細気管支炎3例,気管支拡張症2例,慢性気管支炎2例,過敏性肺臓炎2例であり,昨年度と異なり肺結核がやや多かったことが特徴的であった。(2)これら85症例のうち,温泉療法を受けた症例は63例(74.1%)であった。(3)温泉療 法を受けた症例の地域分布では,鳥取県からの入院症例51例では32例(62.7%)であり,同様に岡山県からの入院症例20例では19例(95.0%),その他の県からの入院症例14例で12例(85.7%)であった。4.温泉療法の臨床効果は,気管支喘息では51例中著効14例,有効27例,やや有効8例,無効2例であり,明らかな有効例は41例(80.3%)であった。
Abstract Alternative Backgrounds and immunological characteristics were studied in patients with chronic respiratory diseases admitted at Misasa Branch Hospital in 1989. At the same time, clinical effects of spa therapy were evaluated for these patients. 1. Eighty five patients with chronic respratory diseases comprised 65 patients with bronchial asthma, 4 with pulmonary emphysema, 4 with lung tuberculosis, 3 with allergic granulomatous angitis, 3 with obstructive bronchiolitis, 2 with bronchiectasia, 2 with chronic bronchitis and 2 with hypersensitivity pneumonitis. 2. Sixty three patients (74.1%) out of the 85 cases had spa therapy. 3. Out of 51 patients coming from Tottori prefecture, 32 cases (62.7%) received spa therapy. On the other hand, spa therapy was carried out for 19 cases (95.0%) out of the 20 cases from Okayama prefecture, and for 12 cases (85.7%) out of the 14 cases from the other prefectures (long distant areas). 4. Many cases with bronchial asthma showed low serum levels of IgE (lower than 200 IU/ml), and frequency of positive skin reactions to various allergens was low in the patients with bronchial asthma. Serum cortisol levels were very low in patients with steroid-dependent asthma. 5. Spa therapy was effective in 41 cases (80.3%) out of the 51 patients with bronchial asthma. Spa therapy also effective for patients with obstructive bronchiolitis, and allergic granulomatous angitis.
Keywords 気管支喘息 (Bronchial asthma) 閉塞性細気管支炎 (Obstructive bronchiolitis) 慢性気管支炎 (Chronic bronchitis) 血清コーチゾール値 (Serum cortisol level) 温泉療法 (Spa therapy)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 7
End Page 15
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002313875
JaLCDOI 10.18926/11670
Title Alternative A case of atopic asthma characterized by marked neutrophilia in BALF
FullText URL 061_129_133.pdf
Author Kawauchi, Kazuhisa| Mifune, Takashi| Kitani, Hikaru| Sudo, Michiyasu| Tanizaki, Yoshirou| Kataoka, Hisakazu| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract 気管支肺胞洗浄液(BALF)中に高度の好中球の増多が見られ,治療により臨床症状の軽快と共にBALF中の好中球の減少を認めたアトピー型喘息の一例を経験した。経過中5回施行したBALF中の好中球百分率はそれぞれ65.4%,56.2%,42.4%,5.6%,5.6%であり,4回目,5回目では著明な好中球減少を認めた。本症例の如き症例は今後増加することが予想され,その発症病態を含め今後さらに症例を重ねて検討して行く必要があると 考えられた。
Abstract Alternative The clinical course and characteristics of a case with atopic asthma, showing marked neutrophilia in BALF and severe asthma attacks, were observed the frequency of neutrophils in BALF decreased followed by the improvement of her asthma attacks with therapy. The frequency of neutrophils in BALF was 65.4% at the initial stage before the therapy and decreased to 5.6% at attackfree stage after the therapy. The changes in frequency of neutrophils in BALF, obserbed in this case, should be noticed, because the cell in BALF were hardly affected by any treatment.
Keywords アトピー型喘息 (Atopic asthma) 好中球 (Neutrophil) 気管支肺胞洗浄法 (Bronchoaiveolar lavage)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 129
End Page 133
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002313690
JaLCDOI 10.18926/11674
Title Alternative Development of stepping measurement device for evaluation of and training in walking
FullText URL 061_123_128.pdf
Author Matsumoto, Shuji| Ochi, Koji| Tanaka, Juntaro| Tachibana, Hideo| Seno, Toshinobu| Harada, Hideo| Tsuboi, Keiichi| Miyake, Hirofumi| Kimura, Ikuro|
Abstract 急性膵炎の回復後その発症原因の検索において発見された小膵癌の3例を報告し,膵炎の発症原因の一つとして膵癌を常に念頭におく必要があることを強調した。またスクリーニング検査および精査において小膵癌を診断する手順について考察を加えた。症例1は初回発作の回復後に,症例2および症例3は再発発作の回復後に急性膵炎の発症原因の検索を目的に紹介された。いずれの症例においても血清腫瘍マーカーは正常植を示し,腹部USおよびCTは腫瘍から尾側の膵管の拡張を示したが腫瘍そのものは描出はできなかった。症例1ではERCP像から膵体部癌を強く疑い,症例2と症例3ではERCP像と細胞診陽性所見から膵頭部癌と確診し,手術を行った。腫瘍の最大径は症例1では0.9cm,症例2では1.5cm,症例3では2.0cmであり,いずれも転移を認めず根治切除可能であった。
Abstract Alternative Although gait training equipment such as the bicycle ergometer and treadmill exists for patients whose walking ability is high, there is no appropriate gait training mehtod or training instrument for patients whose walking ability has become impaired, who often use a cane or walker, etc. in the course of daily life. In the case of gait training for persons whose walking ability involves impaired locomotion, there is always the danger of a fall. Consequently, a caregiver is required, and the effect of the training is cut by half because the patient's anxiety about falling is exacerbated. Slow stepping affords strengthening and balance training of the leg muscles for patients whose walking ability has become low, and walking ability is improved. However,whether such training appropriately carries out stepping and the degree of the effect of such training has not been evaluated. Therefore, we have developed a stepping measturement device that monitors stepping for evaluation and training of walking ability. This system consists of two mat switches for stepping, a measuring circuit for stepping detection, and a book-sized personal computer with a PC card-type AD converter. This system can detect a left or right single stance phase and a double stance phase relative to the ON, OFF condition of the mat switch. After measurement, the following items are analyzed and displayed : ・number of steps, ・average time of double stance phase, ・the average time of single stance phase, and so on. Finally, we measured the stepping of subjects whose walking ability is low, and showed the relationship between daily walking conditions and stepping conditions. The effectiveness of this system was considered in light of the results.
Keywords 小膵癌 (Small pancreatic cancer) 膵癌の早期発見 (Early diagnosis of pancreatic cancer) 急性膵炎 (Acute pancreatitis)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 123
End Page 128
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002313750
JaLCDOI 10.18926/11691
Title Alternative Clinical significance of eosinophilic leucocytes in onset mechanisms of bronchial asthma
FullText URL 061_089_097.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Kawauchi, Kazuhisa| Mifune, Takashi| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract 末梢血好酸球が気管支喘息の発症病態にどのように関与しているかについて,1.末梢血好酸球の変動,2.好酸球の局所出現,3,好酸球とアレルギー反応,4.アレルギー反応と好酸球の機能,5.好酸球と喘息の臨床病態の各項目で検討した。その結果,気管支喘息では,全般的に末梢血好酸球増多がみられ,同時にアレルギー反応局所への遊走が観察されること,末梢気 道では,ある程度肺胞マクロファージの出現と逆相関にあること,リンパ球との関連もある程度認められることなどが示された。そして,局所出現した好酸球は,IgE抗体の関与の程度により,その機能が異なる可能性が示唆された。また臨床病型別の検討では,アトピー型ではいずれの臨床山型においても好酸球の出現率は明かな増加傾向を示したが,非アトピー型では,Ib.気管支攣縮+過分二型においてのみ好酸球増多が著明であった。
Abstract Alternative Participation of eosinophil leucocytes in onset mechanisms of bronchial asthma was discussed in following sections ; 1. changes in number of eosinophils in the peripheral blood, 2. emergence of eosinophils in local allergic reaction sites, 3. eosinophils and allergic reactions, 4. different functions of eosinophils III allergic reactions, 5. eosinophils and clinical types of bronchial asthma. The results showed that eosinophilia in the peripheral blood was generally observed in patients with bronchial asthma. In these cases, eosinophils migrated into local allergic reaction sites. A reversed correlation was present between number of eosinophils and macrophages, and number of eosinophils correlated to a certain extent with number of lymphocytes in the small airways and alveolar region. Function of eosinophils was shown to be different between IgE-and non-IgE-mediated reactions, by measurement of arylsulfatase activity III BALF. Marked eosinophilia in BALF was observed in any clinical types of atopic asthma, although eosinophils were increased only in bronchoconstriction + hypersecretion type (type II) of non-atopic asthma.
Keywords 好酸球 (Eosinophils) 肺生検 (Lung biopsy) 気管支肺胞洗浄 (Bronchoalveolar lavage) 臨床病型 (Clinical type) 気管支喘息 (Bronchial asthma)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 89
End Page 97
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002313427
JaLCDOI 10.18926/11822
Title Alternative Sarcoidosis and Propionibacterium acnes
FullText URL 003_019_036.pdf
Author Nakata, Yasunari| Kataoka, Mikio| Kimura, Ikuro|
Abstract Although there have been numerous reports on the isolation of bacteria, fungus, and aetiological agents of chemical substances from specimens of patients with sarcoidosis, none of them have been substantiated. In any event, an understanding of the pathogenesis of pulmonary sarcoid is intimately linked to that of the processes involved in the accumulation of T-cells in the lower respiratory tract of individuals with sarcoidosis. Propionibacterium acnes was isolated at high rates and in high concetrations from lymph nodes in patients with sarcoidosis. However, the precise mechanism of granuloma formation and immunomodulation by P. acnes has not been elucidated yet. In patients with sarcoidosis, it was found that the high levels of interleukin-2 (IL-2) released from alveolar lymphocytes as well as interleukin-1, tumor necrosis factor (TNF) and interleukin-6 (IL-6) released from alveolar macrophages were stimulated by P. acnes. These cytokines (mainly IL-2), released by P. acnes in large quantities, play a major role in the compartmentalization of the T-cell population in the lung and lead to the formation of an alveolitis and granuloma in the lung parenchyma of patients with sarcoidosis. This paper focuses primarily on the role of the cytokine network in the pulmonary mononuclear cells in the lung of patients with sarcoidosis.
Keywords sarcoidosis macrophage T-cell interleukin-2 Propionibacterium acnes
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 19
End Page 36
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313339
Author Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Soda, Ryo| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Published Date 1993
Publication Title 岡山医学会雑誌
Volume volume105
Issue issue5-6
Content Type Journal Article
JaLCDOI 10.18926/14493
Title Alternative Motility of basophilic granulocytes and histaminerelease
FullText URL 062_047_051.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Nakagawa, Saburo| Soda, Ryo| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract 各種刺激物質添加時の好塩基球の形態的変化―運動亢進と膨化,脱顆粒―と化学伝達物質遊離との関連を中心に若干の知見をのべた。まず,抗原刺激時には,(1)運動亢進をきたし,洋梨状を示す好塩基球と膨化,脱顆粒を示す好塩基球の2種類が観察されること,(2)アトピー型喘息の好塩基球は健康人と比べて全般的に運動が抑制されていること,(3)運動亢進の状態としては,random movementとoriented movementがあり,抗原に対応した特徴的な運動亢進はoriented movementであること,(4)運動亢進と膨化,脱顆粒は,連続した一連のものではなく,それぞれ独立した過程を持っていること,などが観察されている。また,刺激物質の種類によって,好塩基球の形態的変化や化学伝達物質遊離の状態が異なることについても報告した。
Abstract Alternative Morphological changes of basophilic leucocytes from atopic asthmatics were observed in relation to histamine release after the cells were challeuged by a specific allergen, anti-IgE and Ca ionophore A 23187 . 1. Increase in motility and degranulation of basophilic granulocytes were observed after the stimulation with antigen and anti-IgE. The incidence of basophils with oriented movement was siguificantly increased at 36 min and reached the peak at 12-15 min after addition of antigen. 2. A decreased number of basophils, increased cell diameter and increased ratio of the short to long axis diameters were observed with a significant amount of histamine release after antigen and anti-IgE stimulation. 3. Compared to cell reactions to antigen and anti-IgE, Ca ionophore A 23187 stimulation showed more decreased motility and greater increase in the diameter of the cells.
Keywords 好塩基球 (Basophils) 抗原 (Antigen) 運動亢進 (lncreased motility) 脱顆粒 (Degranulation) ヒスタミン遊離 (Histamine release)
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 47
End Page 51
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002308512
JaLCDOI 10.18926/15298
Title Alternative ステロイド依存性重症難治性喘息に対する複合温泉療法の作用機序
FullText URL 063_001_006.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Takatori, Akimasa| Okuda, Hiroyuki| Ochi, Koji| Harada, Hideo| Kimura, Ikuro|
Abstract Effects of complex spa therapy, which comprises swimming training in a hot spring pool, inhalation of iodine salt solution and fango therapy, were analyzed in patients with steroid-dependent intractable asthma (SDIA) by comparison between the subjects with and without efficacy of the therapy. 1. The complex spa therapy was effective in the patients with lower levels of FEV(1.0%) and % PEFR. 2. The complex spa therapy was not always effective in the patients with a low value of % V(25), which represents depressed airflow in the small airways. 3. The efficacy of the complex spa therapy was found in the patients showing a low value of % V(25) accompanied by BAL neutrophilia, but not found in those without increased proportion (more than 10% ) of neutrophils in BAL fluid. The results reveal that the complex spa therapy is effective in patients with SDIA showing low values of FEV(1.0%) and % PEFR, and in those with a low value of % V(25) and BAL neutrophilia.
Abstract Alternative ステロイド依存性重症難治性喘息を対象に,温泉プール水泳訓練,ヨードゾル吸入療法.鉱泥湿布療法からなる複合温泉療法の臨床効果の解析を行った。1.複合温泉療法は,FEV(1.0%)および% PEFR値の低い症例により有効であった。2.複合温泉療法は、細気管支領域の換気障害を示すと考えられる% V(25)値が低い症例に対して,必ずしも有効ではなかった。3.複合温泉療法の効果は,BAL液中好中球増多(10%以上)が見られ、かつ% V(25)値が低い症例により有効であったが、同様に% V(25)値が低くても,BAL液中に好中球増多 が見られない症例に対しては,有効ではなかった。以上の結果より,複合温泉療法はFEV(1.0%)や% PEFR値の低く,またBAL液中好中球増多が見られ,かつ% V(25)値が低いようなステロイド依存性重症難治性喘息に対して有効性が高いことが示唆された。
Keywords complex spa therapy (複合温泉療法) ventilatory function (換気機能) BAL neutrophilia (BAL好中球増多) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1992-06
Volume volume63
Start Page 1
End Page 6
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002307537
JaLCDOI 10.18926/15299
Title Alternative ステロイド依存性重症難治性喘息に対する温泉療法の重要性。過去10年間の181例を対象に。
FullText URL 064_001_010.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Kawaraya, Masashi| Ochi, Koji| Harada, Hideo| Tada, Shinya| Kimura, Ikuro|
Abstract Characteristics of 181 patients with steroid-dependent intractable asthma (SDIA) who were admitted to our hospital and had spa therapy for last 10 years were examined in relation to the methods of spa therapy. For last 10 years, three kinds ofspa therapies were administered: spa therapy A (swimming training in a hot spring pool) in the first atage (1982-1985), spa therapy B (swimming training + inhalation of iodine salt solution) in the middle stage (1986 -1989), and spa therapy C (swimming training + inhalation of iodine salt solution + fango therapy) in the last stage (1990-1991) of this study. 1. The number of patients with SDIA who were admitted to our hospital and had spa therapy tended to increase from 2 in 1986 to 35 in 1991. 2. The number of SDIA patients over the age of 60 or over the age at onset of 40 tended to increase from the first stage to the last stage. 3. The proportion of patients from distant areas (prefectures other than Tottori) was decreasing for the last stage, although the number of these patients was increasing in recent years. 4. The proportions of patients divided by serum IgE levels and by clinical asthma types did not change for last 10 years. 5. The proportion of type Ib and type II asthma was considerably high in patients with SDIA for last 10 years. 6. Clinical effects of spa therapy were observed in 21 of the 30 (70.0%)patients with SDIA in the first stage (1982-1985) with spa therapy A, 68 of the 83 (74.7%) patients with SDIA in the middle stage (1986-1989) with spa therapy B, and 61 of the 68 (89.7%) patients with SDIA in the last stage (1990-1991) with spa therapy C.
Abstract Alternative 最近10年間に三朝分院へ入院し,温泉療法を受けたステロイド依存性重症難治性喘息181例を対象に,温泉療法の方法との関連のもとに,対象患者の背景について検討を加えた。最近10年間に3種類の温泉療法が行われた。温泉療法A(温泉プール水泳訓練)は初期(1982-1985),温泉療法B(水泳訓練+ヨードゾル吸入)は中期(1986-1989),また,温泉療法C(水泳訓練+ヨードゾル吸入+鉱泥湿布療法)は後期(1990-1991)に行われた。1.当院へ入院し,温泉療法を受けたステロイド依存性重症難治性喘息(SDIA)患者の数は,1986年の2例から1991年の35例へと増加の傾向を示した。2.SDIA患者のうち,60才以 上の症例および40才以降の発症症例の数は,1986年以降急激な増加傾向を示した。3.鳥取県以外の他県(遠隔地)からの入院患者の頻度は,近年減少の傾向を示したが,その絶対数は増加の傾向であった。4.血清IgE値はいずれの時期においても200IU/mℓ以下の症例が多い傾向が見られた。5.臨床病型では,通常の喘息に比べIb型およびII型の頻度が高い傾向が見られた。6.温泉療法の臨床効果は,温泉療法Aが行われた初期では70.0%,温泉療法Bの中期では74.7%,温泉療法Cの後期では89.7%であった。
Keywords swimming training in a hot spring pool (温泉プール水泳訓練) inhalation of iodine salt solution (ヨードゾル吸入) fango therapy (鉱泥湿布療法) SDIA
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 1
End Page 10
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307391
JaLCDOI 10.18926/15301
Title Alternative 気管支喘息における副腎皮質ホルモン長期投与による免疫抑制と気管支肺胞洗浄液中好中球増多との関連
FullText URL 064_017_023.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Kawaraya, Masashi| Harada, Hideo| Kimura, Ikuro|
Abstract Cellular composition of bronchoalveolar lavage (BAL) fluid was compared between two age-matched steroid-dependent intractable asthma (SDIA) groups : 17 group A patients (serum IgG level < 1000mg/dℓ) whose immunity was regarded as being suppressed by long-term glucocorticoid therapy and 17 group B patients (serum IgG level > 1200mg/dℓ). 1. The levels of serum IgA and IgM were significantly decreased in group A compared to the levels in group B patients. 2. The number of blood lymphocytes was also significantly lower in group A than in group B patients. However, the number of blood neutrophils was not different between the two asthma groups. 3.The number of BAL lymphocytes was significantly lower in group A than in group B patients. 4. BAL neutrophilia (more than 1.5×10 (4)/mℓ) was observed in 6 of the 17(35.3%) patients in group A, but not observed in any patients in group B. The number of BAL neutrophils was significantly greater in group A than in group B patients. These results suggest that there is a correlation between immunosuppression by glucocorticoids and BAL neutrophilia in patients with bronchial asthma.
Abstract Alternative ステロイド依存性重症難治性喘息を,免疫抑制の見られる症例(血清IgG値1000mg/dℓ以下)(グループA)と抑制の見られない症例(血清IgG値1200mg/dℓ以上)(グループB)の2群に分け,気管支肺胞洗浄液中の細胞成分について比較検討した。1.血清IgAおよびIgM値は,グループBに比べ免疫抑制の見られるグループAにおいて有意に低い値であった。2.末梢血リンパ球数は,グループAにおいて,グループBに比べ有意に少ない傾向が見られた。しかし,末梢血好中球数は両群間に有意の差は見られなかった。3.BAL液中リンパ球数は,グループBに比べ免疫抑制の見られるグループAにおいて有意に少 ない数であった。4.BAL液中好中球増多(1.5×10(4)/mℓ以上)を示す症例は, グループAの17例中6例(35.3%)に見られたが,グループBでは全く 見られなかった。また,BAL液中好中球数は,グループAにおいてグループBに比べ有意に多い数であった。以上の結果により,副腎皮質ホルモン長期投与 による免疫抑制とBAL液中好中球増多とはある程度関連があることが明らかにされた。
Keywords immunosuppression (免疫抑制) BAL neutrophilia (BAL好中球増多) glucocorticoids (副腎皮質ホルモン) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 17
End Page 23
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307882
JaLCDOI 10.18926/15302
Title Alternative 気管支喘息における気道内リンパ球の出現とその意義.IgE系反応との関連について.
FullText URL 063_019_024.pdf
Author Tanizaki, Yosihiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Tanimizu, Masakuni| Honke, Naoko| Kusaura, Yasuhiro| Kimura, Ikuro|
Abstract Relationship of the proportion of lymphocytes in bronchoalveolar lavage (BAL) fiutd to IgE-mediated reactions and to ventilatory function was studied in two age-matched asthma groups : group A of 11 subjects with BAL lymphocytes over 30% and group B of 11 subjects with BAL leyphocytes less than 10%. Of the eleven subjects in group A, 3 (27.3%) were atopic and 4 (36.4%) were non-atopic. The proportion of BAL lymphocytes in subjects with serum IgE level more than 300 IU/ml, who were all older than 50 years, was significantly higher than that in subjects with serum IgE less than 100 IU/ml in group A (p<0.05). While of the eleven subjects of group B, 5 (45.5%) were atopic and 4 (36.4%) were non-atopic. The value of % V(25) was significantly higher in the atopics compared with the non-atopics in group A (p<0.05), although no significant difference was found in the value of % V(25) between the two asthma types in group B. The results show that an increased number of lymphocytes in BAL fluid may be enhanced by IgE-mediated allergic reactions in the elderly, and that the decreased value of % V(25) may be associated withan increased number of lymphocytes in BAL fluid in non-atopics.
Abstract Alternative 気管支喘息を対象に,気管支肺胞洗浄液(BALF)中のリンパ球の出現頻度と,IgE系反応および換気機能との関連について検討を加えた。対象は,同年齢の2つのグループ,すなわち,BALF中リンパ球出現頻度30%以上の症例(グループA)と10%以下の症例(グループB)に分けて,それぞれ11例ずっで比較検討を行った。グループAでは,11例中3例がアトピー型,4例が非アトピー型であった。血清IgEが300IU/ml以上の症例(いずれも50才以上)では,100IU/ml以下の症例に比べ,BALF中リンパ球の出現頻度は,有意に高い傾向を示した。一方,グループBの11例では,アトピー型5例,非アトピー型4例であった。% Ⅴ(25)値は,グループAではアトピー型で,非アトピー型に比べ有意に高い値を示した。これらの結果は,50才以上の症例では,IgE系の反応がリンパ球の出現頻度を増強させること,そして,非アトピー型では,リンパ球の出現頻度と% Ⅴ(25)笛値との間にある程度の関連が見られることを示唆しているものと考えられた。
Keywords Lymphocytes (リンパ球) BAL fluid (BAL液) IgE antibody (IgE抗体) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1992-06
Volume volume63
Start Page 19
End Page 24
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002307202
JaLCDOI 10.18926/15303
Title Alternative 気管支喘息におけるActivated Tリンパ球について,喘息発作との関連
FullText URL 064_011_016.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Kawaraya, Masashi| Harada, Hideo| Tada, Shinya| Kimura, Ikuro|
Abstract The number of CD4(+) T-Iymphocytes, CD4/CD8 ratio, and the number of IL2R(+) T-Iymphocytes (CD25) were examined in 14 patients with bronchial asthma, and the results were compared in three different asthma stages : symptom-free stage, wheeze stage, and attack stage. 1. The proportion of blood CD4(+) T-lymphocytes was more decreased in patients with asthma attacks than in those without symptoms. The CD4/CD8 ratio was also more decreased in patients with attacks than in those without symptoms. However, these differences were not significant. 2. The proportion of blood activated T cells (IL2R(+) cells, CD25) was significantly increased in patients with attacks than in those without symptoms. The proportion of activated T cells in 5 asthma patients was 2.9±0.8% in symptom-free stage and 6.6±1.1% in attack stage(p<0.001). These findings show that numbers of activated T-lymphocytes in peripheral blood were increased during asthma attacks, and this increase suggests the participation of activated T cells in the pathogenesis of asthma.
Abstract Alternative 気管支喘息14例を対象に,末梢血CD4(+)リンパ球の頻度,CD4/CD8比およびIL2R(+)Tリンパ球の頻度について,喘息発作との関連のもとに検討を加えた。1.CD4(+)リンパ球の頻度は,非発作時の症例で最も高く,喘鳴のみの症例,発作の見られる症例へと順次低くなる傾向が見られたが,推計学的には有意の差は見られなかった。CD4/CD8比も同様に,非発作時に最も高く,発作を有する症例では非発作時に比べ低い値を示したが,推計学的には有意の差は見られなかった。2.IL2R(+)Tリンパ球(activated Tリンパ球)の頻度は,非発作時の症例で最も低く(3.5±1.1%),喘鳴のみをともなう症例(3.8±1.1%),発作の見られる症例(5.8±1.9%)へと順次高くなる傾向を示し,発作のある症例では,非発作および喘鳴のみの症例に比べ有意に高い値 を示した。また,非発作時と発作のある時期にIL2R(+)Tリンパ球を観察し得た5症例では,非発作時2.9±0.8%,発作のある時期6.6±1.1%であり,発作のある時期に有意の高値を示した(p<0.001)。これらの結果は,発作のある時期にはactivated Tリンパ球の頻度が増加すること,そして,この増加はactivated Tリンパ球が喘息発作の病態と密接な関連を有していることを示唆するものと考えられた。
Keywords CD4(+) T cells (CD4(+)リンパ球) CD4/CD8 ratio (CD4/CD8比) IL2R(+) T cells (IL2R(+)Tリンパ球) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 11
End Page 16
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307394
JaLCDOI 10.18926/15304
Title Alternative 気管支喘息における気道内への好酸球の出現とその意義. 過分泌と関連して.
FullText URL 063_014_018.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Kimura, Ikuro|
Abstract A correlation between the proportion of eosinophils in bronochoalveolar lavage (BAL) fluid and amount of expectoration was examined in patients with atopic asthma. The subjects were divided into two groups : cases with high proportion (20% of more) (group A) and low proportion (less than 5%) (group B) of eosinophils in the BAL fluid. Any significant difference was not paesent in the value of each ventilatory parameter between the two groups. The amount of expectoration/day was significantly higher in group A compared with group B (p<0.001). These results suggest that eosinophilia in BAL fluid correlates to mucus hypersecretion in the airways of patients with atopic asthma.
Abstract Alternative アトピー型気管支喘息のなかから,気管支肺胞洗浄液(BALF)中の好酸球の出現頻度が20%以上の症例(グループA)と5%以下の症例(グループB)の2群を選び出し,それぞれの換気機能および喀痰量を比較検討した。その結果,換気機能には両群間に有意の差は見られなかったが,1日喀痰量は,BALF中好酸球の著明増多を示すグループAにおいて有意に多い傾向が見られた。これらの結果は,BALF中好酸球増多と過分泌との間にはある程度の関連があることを示しているものと考えられた。
Keywords Eosinophilia (好酸球) Hypersecretion (過分泌) BAL fluid (BAL) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1992-06
Volume volume63
Start Page 14
End Page 18
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002307156
JaLCDOI 10.18926/15305
Title Alternative 気管支喘息における気道炎症反応の臨床的意義。慢性閉塞性細気管支炎との比較検討
FullText URL 064_024_030.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Kawaraya, Masashi| Ochi, Koji| Harada, Hideo| Tada, Shinya| Kimura, Ikuro|
Abstract Clinical significanoce of airway inflammation was assessed in 28 patients with bronchial asthma, and the results were compared with those in 9 patients with chronic obstructive bronchiolitis (COB). 1. Total cell number in bronchoalveolar lavage(BAL) fluid was markedly increased in patients with COB (51.0×10(6)), which was significantly greater than that in those with bronchial asthma (9.6×10(6) ). 2. The proportion of BAL macrophages was significantly higher in patients with bronchial asthma than in those with COB. However, the, absolute number of BAL macrophages was not significantly different between them. 3. The proportion of BAL lymphocytes was higher in patients with bronchial asthma than in those with COB, but this difference was not signifcant. In contrast, the absolute number of BAL lymphocytes was significantly greater in patients with COB than in those with bronchial asthma. 4. The proportion and the number of BAL neutrophils were markedly increased in patients with COB compared to those with bronchial asthma. 5. The proportion and the number of BAL eosinophils were higher in patients with bronchial asthma than in those with COB, although these defferences were not significant. The results suggest that airway inflammation in bronchial asthma is not so intensive as that in COB.
Abstract Alternative 気管支喘息28例および慢性閉塞性細気管支炎9例を対象に,気道炎症反応の臨床的意義について検討を加えた。1.気管支肺胞洗浄(BAL)液中の総細胞数は,慢性閉塞性細気管支炎において著明に増加しており(51.0×10(6)),気管支喘息(9.6×10(6))との間に有意の差が見られた。2.BAL液中マクロファージの頻度は気管支喘息において,慢性閉塞性細気管支炎に比べ有意に高い値を示したが,絶対数での比較では両疾患群間に有意の差は見られなかった。 3.BAL液中リンパ球の頻度は,気管支喘息においてやや高い傾向が見られたが,絶対数の比較では慢性閉塞性細気管支炎において有意に高い値であった。 4.BAL液中の好中球は,その頻度および絶対数とも慢性閉塞性細気管支炎において著明に増加しており,これは気管支喘息と比べ有意に高い値であった。 5.BAL液中好酸球の頻度および絶対数は,気管支喘息においてやや高い値であったが,両疾患群間に有意の差は見られなかった。以上の結果より,気管支喘息における気道炎症反応は,慢性閉塞性細気管支炎ほど高度ではない ことが明らかにされた。
Keywords airway inflammation (気道炎症反応) bronchial asthma (気管支喘息) chronic obstructive bronchiolitis (慢性閉塞性細気管支炎) lymphocytes (リンパ球) neutrophils (好中球)
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 24
End Page 30
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307730
JaLCDOI 10.18926/15306
Title Alternative 気管支喘息における気道内への好中球の出現とその意義 換気機能と関連して
FullText URL 063_025_029.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Tanimizu, Masakuni| Honke, Naoko| Kusaura, Yasuharu| Kimura, Ikuro|
Abstract Ventilatory function was compared between two age-matched asthma groups : group A (study group) consists of 8 patients with bronchial asthma with marked neutrophilia (20% or more) in the BAL fluid and group B (control group) of 8 patients with neutrophils (less than 5% of total BAL cells). The values of six ventilatory parameters were lower in group A than in group B, and a significant difference was found in FEV(1.0%), % MMF, % V(50) and % V(25) between groups A and B. Of these parameters, the value of % V(25) was markedly decreased in group A compared with group B. The results show that ventilatory dysfunction in airways, especially in small airways, is closely correlated to increased number of neutrophils in BAL fluid.
Abstract Alternative 気管支喘息を対象に,年令をmatchさせた2つのグループ,すなわち,気管支肺胞洗浄液(BALF)中の好中球の出現頻度が20%以上の8症例(グループA)と,BALF中の好中球の出現頻度が5%以下の8症例(グループB)における換気機能について比較検討を行った。6つの換気パラメーターは,全般的にグループAにおいてグループBに比べ低い傾向が見られ,FEV(1.0%),% MMF,% V(50)および% V(25)値は,いずれもグループAにおいて有意に低い値が示された。これらのパラメーターのうち,% V(25)値はグループBに比べグループAにおいて著明に低い値が示された。これらの結果は,気道における換気障害,特に小ないし細気管支領域の換気障害は,BALF中の好中球の出現頻度とある程度関連していることを示すものと考えられた。
Keywords ventilatory function (換気機能) neutrophils in BAL fluid (好中球, BAL) small airways (細気管支) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1992-06
Volume volume63
Start Page 25
End Page 29
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002307993
JaLCDOI 10.18926/15307
Title Alternative 気管支喘息におけるハウスダストアレルギー. 血清IgE値とIgE抗体価と年齢との関連
FullText URL 064_031_035.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Ochi, Koji| Harada, Hideo| Tada, Shinya| Kimura, Ikuro|
Abstract Serum IgE levels and IgE antibody values in relation to patient age were examined in 50 patients with house dust mite (HDm) sensitive asthma, 1. IgE antibody values to HDm were highest in patients between the ages of 10 and 19, and the values generally low in those over the age of 40. 2. High IgE antibody values to HDm were accompanied by high serum IgE levels in patients between 10 and 19. IgE antibody values and serum IgE levels were in general low in patients over age 40. In patient under age 9, serum IgE levels were less than 500 IU/ml in 5 of the 10 subjects despite an increased value of IgE antibodies to HDm. These results suggest that an increased production of IgE antibodies to HDm is usually accompanied by an increased production of total IgE in younger subjects. However, an increase in production of IgE antibodies is not always related to an increase in production of total IgE in patients under age 9. The results also show that production of IgE antibodies to HDm and total IgE is in general low in older patients despite the hyperreactivity to HDm.
Abstract Alternative ハウスダストが原因抗原であるアトピー型気管支喘息50例を対象に,血清IgE値とIgE抗体価を年齢との関連のもとに検討した。1.IgE抗体価は10-19才の年齢層の症例において最も高く,一方40才以上の症例では全般的に低い傾向が見られた。2.10-19才の年齢層の症例では,IgE抗体価の高値と同時に血清IgE値も高い値を示したが,40才以上の症例では,IgE抗休価および血清IgE値いずれも10-19才の症例に比べ全般的に低い値を示した。9才以下の症例では,IgE抗体価の高値にもかかわらず,血清IgE値は比較的低く,500IU/ml以下を示した症例が10例中5例に観察された。これらの結果は,若年症例では,IgE抗体の産生亢進と総IgEの産生亢進が同時に見られること が多いが,9才以下ではIgE抗体産生亢進に総IgE産生亢進が必ずしもともなわない場合があることを示している。また,40才以上の症例では,ハウスダストに対する過敏性は有しているものの,IgE抗体価および血清IgEは全般的に低い値を示す傾向が示唆された。
Keywords Serum IgE (血清IgE) IgE antibodies (IgE抗体) house dust mite (ダニ) bronchial asthma (気管支喘息)
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 31
End Page 35
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307674
JaLCDOI 10.18926/15308
Title Alternative 老年者気管支喘息における液性および細胞性免疫反応について
FullText URL 064_075_082.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Kawaraya, Masashi| Ochi, Koji| Harada, Hideo| Tada, Shinya| Kimura, Ikuro|
Abstract Humoral and cellular immune responses in bronchial asthma were examined by comparing the results of older asthmatics (group OA) with those of older healthy subjects (group OH) and of younger asthmatics (group YA). In humoral immune response, IgE-mediated immune system expressed by elevated serum IgE levels and IgE antibodies for house dust mite (HDm) and/or Candida albicans was clearly found in older asthmatics, while there was no finding showing the presence of IgE-mediated immune response in older healthy subjects. There was no significant difference in levels of serum IgG and IgA among the three groups. However, the serum IgM level was significantly lower in older asthmatics than in younger asthmatics. IgE antibodies to HDm and/or Candida albicans were more frequently found in older asthmatics compared with older healthy subjects. In contrast, IgG(4) antibodies to HDm were not different among the three groups. IgG(4) antibodies to Candida albicans were significantly higher in both older groups than in younger group. However, there was no significant difference between asthmatic and healthy subjects in the elderly. Delayed skin reaction to PPD was significantly more decreased in older asthmatics. These results show that bronchial asthma in the elderly is characterized by the presence of IgE-mediated immune response and by the changes of immune response with aging.
Abstract Alternative 気管支喘息における液性および細胞性免疫反応について,老年者気管支喘息,老年者健常人,および若年者気管支喘息の間で比較検討を行った。1.まず,液性免疫では,血清IgE値高値,ハウスダストやカンジダに対するIgE抗体陽性などのIgE系反応は,老年者気管支喘息においては明らかに観察されたが,老年者健常人では全く観察されなかった。血清IgGおよびIgA値には3者間に有意の差は見られなかったが,血清IgMは,若年者喘息に比べ老年者喘息で有意に低い値を示した。2.ハウスダストやカンジダに対するIgE抗体は老年者喘息において老年者健常人に比べより高い頻度で陽性であったが,一方,ハウスダストに対するIgG(4)抗体は3者聞に有意の差は見られなかった。また,カンジダに対するIgG(4)抗体は,老年者喘息および健常人において,若年者喘息に比べ有意の高値を示した。しかし,老年者の喘息と健常人との間には有意の差は見られなかった。3.PPDによる遅延型皮膚反応は,若年者喘息に比べ老年者喘息において有意の減弱傾向を示した。これらの結果は,老年者喘息はIgE系反応を有していること,そして,加齢による免疫反応の変化の影響を受けること,を示している。
Keywords IgE IgG(4) Immunoglobulings (免疫グロブリン) PPD Bronchial asthma (気管支喘息) 加齢
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 75
End Page 82
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307261
JaLCDOI 10.18926/15310
Title Alternative 気管支喘息の臨床分類とその細胞性および液性因子の特徴
FullText URL 064_036_042.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Sugimoto, Keisuke| Yokota, Satoshi| Hiramatsu, Junichi| Kawaraya, Masashi| Nakagiri, Yoshiyasu| Tada, Shinya| Kimura, Ikuro|
Abstract Characteristics of each asthma type classified by clinical symptoms and findings were studied in 72 patients with bronchial asthma. 1. Ventilatory function tests showed that the values of % MMF, % V(50) and % V(25) were significantly lower in patients with bronchiolar obstruction (type II) compared to the values of those with simple bronchoconstriction type (type Ia) and those with bronchoconstriction + hypersecretion (type Ib). 2. The proportion of neutrophils in bronchoalveolar lavage (BAL) fluid was significantly higher in type II than in type Ia and type Ib. Several patients with type Ib showed higher proportion of BAL eosinophils. 3. The release of LTC(4) from leucocytes was significantly lower in type II compared with type Ia and type Ib. There was no significant difference in the release of histamine and LTB(4) among the three asthma types.
Abstract Alternative 気管支喘息72例を対象に,その臨床病態の特徴を,気道炎症性の細胞および化学伝達物質の観察により検討した。1.気道反応の特徴を換気機能の面から検討すると,II細気管支閉塞型において,MMF,V(50)やV(25)などの小ないし細気管支領域の換気障害を示すパラメーターの値は,Ia型,Ib型に比べ 有意の低下を示した。2.気道細胞反応では,II型において,他の臨床病型に比べ,BAL液中好中球の出現頻度の有意の増加が観察された(Ia, p<0.001 ; Ib, p<0.01)。好酸球の出現頻度は,Ib過分泌型において著しい増加傾向を示す症例が見られたが,その平均出現頻度には3病型間に有意の差は見られなかった。3.好中球からのメジェー夕ー遊離では,ヒスタミン遊離は,Ia単純性気管支撃縮型において最も高い値が示されたが,推計学的には3病型間に有意の差は見られなかった。ロイコトリエンC(4)では,Ia型においてII型に比べ有意に高い遊離が観察された(p<0.05)。ロイコトリエンB(4)遊離には,3病型間に差は見られなかった。
Keywords Bronchial asthma Asthma classification BAL fluid chemical mediators
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 36
End Page 42
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307662
JaLCDOI 10.18926/15311
Title Alternative 抗原および Ca ionophore A23187 刺激時におけるアトピー型気管支喘息末梢血好塩基球の運動能
FullText URL 064_043_051.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Ochi, Koji| Harada, Hideo| Nakagawa, Saburo| Soda, Ryo| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract We examined histamine release and morphological changes in basophilic granulocytes from atopic subjects, in response to stimulation with antigen and Ca ionophore A23187. 1. Histamine release and a reduction in the rumber of basophils were more rapid and greater in extent at an early stage of antigen stimulation compared with Ca ionophore A23187 stimulation. 2. Morphological changes in basophils, represented by increased motility, in terms of an increased ratio of short to long axis diameter (L/S ratio), as well as the increased frequency of basophils with localized granules and those with pseudopods, were more often observed antigen stimulation than with Ca ionophore A23187 stimulation. In contrast, morphogical changes in which basophils appeared swollen, showing an increased mean cell diameter and an increased frequency of cells with 5 vacuoles or more were more predominant with Ca ionophore A23187 than with antigen stimulation. The results obtained here show that bridging of IgE receptors is essential to activate basophils and induce increased motility in these cells.
Abstract Alternative アトピー型気管支喘息末梢血好塩基球を用い,抗原および Ca ionophore A23187 刺激時のヒスタミン遊離およびその形態的変化について比較検討した。1.抗原刺激時には, Ca ionophore A23187 刺激時に比べ,好塩基球からのヒスタミン遊離および好塩基球数の減少の程度は,より急激でかつ高度であった。2.好塩基球の形態的変化,すなわち,長経/短経比の増大,限局性顆粒あるいは偽足を有する好塩基球の出現頻度の増加などの運動能の亢進を示唆する好塩基球の形態的変化は, Ca ionophore A23187 に比べ抗原刺激時により高度であった。一方,平均直径の増大,5個以上の空砲を有する好塩基球の出現頻度の増加などの,むしろ膨化傾向を示唆する好塩基球の形態的変化は,抗原刺激時に比べ, Co ionophore A23187 刺激時により高度であった。これらの結果より,抗原刺激によるIgE受容体のbridgingが,好塩基球を活性化し,運動能の亢進をひき起こすものと判断された。
Keywords histamine release (ヒスタミン遊離) increased basophil motility (好塩基球の運動能の亢進) bridging of IgE receptors (LgE 受容体) antigen (抗原) Ca ionophore A23187
Publication Title 岡大三朝分院研究報告
Published Date 1993-06
Volume volume64
Start Page 43
End Page 51
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307677