Author 山﨑 修|
Published Date 2002-03-25
Publication Title
Content Type Thesis or Dissertation
Author Yamasaki, Osamu|
Published Date 2007-05-01
Publication Title 岡山医学会雑誌
Volume volume119
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/53118
FullText URL 69_1_17.pdf
Author Suzuki, Norihiro| Takata, Minoru| Shirafuji, Yoshinori| Otsuka, Masaki| Yamasaki, Osamu| Asagoe, Kenji| Hatta, Naohito| Iwatsuki, Keiji|
Abstract Sentinel lymph node (SLN) biopsies have widely been used for the detection of occult LN metastasis of malignant melanoma (MM). In addition to conventional biomarkers, we assessed the diagnostic and prognostic significance of melanoma-initiating cell (MIC) markers in SLNs of MM. We examined the expressions of gp100, MART-1 and tyrosinase mRNA for routine diagnosis and those of ABCB5, CD133, nestin, KDM5B, NGFR and RANK mRNA as MIC markers. The presence of micrometastasis was confirmed immunohistochemically using antibodies to S-100, HMB-45, MART-1, and tyrosinase. Discordance between immunohistochemical and molecular data was observed in 14 of 70 (20.0%) patients, among whom five (7.1%) were positive for only molecular markers;two of these five patients tested positive for micrometastasis by repeated immunohistochemical stainings. The quantitative expression levels of gp100, MART-1, and tyrosinase mRNA were significantly higher in the metastatic LNs;the cut-off values remain to be elucidated. ABCB5 mRNA expression was detected more frequently in the metastatic SLNs (p<0.05) and in the group of patients with recurrence. To make a definite diagnosis of metastasis, we still need a combination of immunohistochemical and molecular probes. ABCB5 might be a suitable molecular marker for the detection of melanoma-initiating cells in SLNs.
Keywords melanoma cancer-initiating cell sentinel lymph node ABCB5
Amo Type Original Article
Published Date 2015-02
Publication Title Acta Medica Okayama
Volume volume69
Issue issue1
Publisher Okayama University Medical School
Start Page 17
End Page 27
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 25703167
Web of Sience KeyUT 000349740300002
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/53114
Author Tada, Kotaro| Hamada, Toshihisa| Asagoe, Kenji| Umemura, Hiroshi| Mizuno-Ikeda, Kazuko| Aoyama, Yumi| Otsuka, Masaki| Yamasaki, Osamu| Iwatsuki, Keiji|
Published Date 2014-11
Publication Title European Journal of Dermatology
Volume volume24
Issue issue6
Content Type Journal Article
Author Sugihara, Satoru| Sugimoto, Saeko| Tachibana, Kota| Kobashi, Mina| Nomura, Hayato| Miyake, Tomoko| Hirai, Yoji| Yamasaki, Osamu| Morizane, Shin|
Keywords Epidermal keratinocyte IL-17A Lympho-epithelial Kazal-type inhibitor Serine protease inhibitor TNF-α
Note This fulltext will be available in Oct 2020|
Published Date 2019-08-21
Publication Title Journal of Dermatological Science
Volume volume96
Issue issue1
Publisher Elsevier
Start Page 26
End Page 32
ISSN 09231811
NCID AA1075636X
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 31501002
DOI 10.1016/j.jdermsci.2019.08.007
Web of Sience KeyUT 000497889600004
Related Url isVersionOf https://doi.org/10.1016/j.jdermsci.2019.08.007
FullText URL JIMHICR2019.pdf
Author Matsuo, Toshihiko| Tanaka, Takehiro| Yamasaki, Osamu|
Keywords BRAF inhibitor BRAF mutation MEK inhibitor PET/CT dabrafenib lacrimal sac malignant melanoma trametinib
Note UNSP 2324709619888052|
Published Date 2019-11-20
Publication Title Journal of Investigative Medicine High Impact Case Reports
Volume volume7
Publisher Sage Publications
Start Page 1
End Page 6
ISSN 2324-7096
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version publisher
PubMed ID 31747798
DOI 10.1177/2324709619888052
Web of Science KeyUT 000498954100001
Related Url isVersionOf https://doi.org/10.1177/2324709619888052