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ID 57307
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Author
Sugihara, Satoru Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sugimoto, Saeko Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Tachibana, Kota Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kobashi, Mina Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nomura, Hayato Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Miyake, Tomoko Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hirai, Yoji Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yamasaki, Osamu Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kakenhi
Morizane, Shin Departments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kakenhi
Abstract
BACKGROUND:
Serine proteases have important roles in skin barrier function and desquamation, and the aberrant expression or the dysfunction of serine proteases is associated with the pathogenesis of skin diseases. Serine protease activities are tightly regulated by serine proteases such as kallikrein-related peptidases (KLKs) and serine protease inhibitors such as lympho-epithelial Kazal-type related inhibitor (LEKTI). For a better understating of diseases' pathogenesis, the regulation mechanism of serine proteases and the inhibitors' expression in epidermal keratinocytes must be clarified.
OBJECTIVES:
To investigate the effects of the cytokines on the expression of LEKTI in epidermal keratinocytes.
METHODS:
Normal human epidermal keratinocytes (NHEKs) were stimulated with panels of inflammatory cytokines. The expression of serine protease inhibitors was analyzed using quantitative real-time PCR and ELISA. LEKTI expression in normal human skin and lesions from psoriasis or atopic dermatitis (AD) were analyzed by immunohistochemically and tape-stripping. Trypsin- and chymotrypsin-like serine protease activities in culture supernatants were measured by using specific substrates.
RESULTS:
TNF-α and IL-17A significantly induced the expression of LEKTI in NHEKs. The immunohistochemical and tape-stripping analysis revealed that psoriatic skin lesions had higher LEKTI expression compared to normal skin and AD lesions. Trypsin- and chymotrypsin-like protease activities in the culture media were upregulated 3-5 days later but attenuated 6-7 days later period by these cytokines.
CONCLUSIONS:
In epidermal keratinocytes, the Th1&Th17 cytokines TNF-α and IL-17A induce the expression of serine protease inhibitor LEKTI, and it might occur to suppress the increase in the serine protease activities under inflammation.
Keywords
Epidermal keratinocyte
IL-17A
Lympho-epithelial Kazal-type inhibitor
Serine protease inhibitor
TNF-α
Note
This fulltext will be available in Oct 2020
Published Date
2019-08-21
Publication Title
Journal of Dermatological Science
Volume
volume96
Issue
issue1
Publisher
Elsevier
Start Page
26
End Page
32
ISSN
09231811
NCID
AA1075636X
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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DOI
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Related Url
isVersionOf https://doi.org/10.1016/j.jdermsci.2019.08.007
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Satoru Sugihara, Saeko Sugimoto, Kota Tachibana, Mina Kobashi, Hayato Nomura, Tomoko Miyake, Yoji Hirai, Osamu Yamasaki, Shin Morizane, TNF-α and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes, Journal of Dermatological Science, Volume 96, Issue 1, 2019, Pages 26-32, ISSN 0923-1811, https://doi.org/10.1016/j.jdermsci.2019.08.007.
Open Access (Publisher)
non-OA
Open Archive (publisher)
Non-OpenArchive