JaLCDOI 10.18926/AMO/49042
FullText URL 66_6_461.pdf
Author Koike, Kazuko| Takaki, Akinobu| Kato, Nobuyuki| Ouchida, Mamoru| Kanzaki, Hirotaka| Yasunaka, Tetsuya| Shiraha, Hidenori| Miyake, Yasuhiro| Yamamoto, Kazuhide|
Abstract Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their “cured” cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression.
Keywords hepatitis C virus retinol-binding protein
Amo Type Original Article
Published Date 2012-12
Publication Title Acta Medica Okayama
Volume volume66
Issue issue6
Publisher Okayama University Medical School
Start Page 461
End Page 468
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 23254580
Web of Science KeyUT 000312966100005
Author Tsutsumi, Koichiro| Kawamoto, Hirofumi| Yamamoto, Kazuhide|
Published Date 2010-12-01
Publication Title 岡山医学会雑誌
Volume volume122
Issue issue3
Content Type Journal Article
Author Iwamuro, Masaya| Ohya, Shogen| Yoshioka, Masao| Nasu, Tatsuyo| Ogawa, Tsuneyoshi| Ito, Mamoru| Ishiyama, Shuhei| Fujiwara, Akiko| Shiode, Junji| Yamamoto, Kazuhide| Itoshima, Tatsuya|
Published Date 2005-09-01
Publication Title 岡山医学会雑誌
Volume volume117
Issue issue2
Content Type Journal Article
Author Yamamoto, Kazuhide|
Published Date 2008-12-01
Publication Title 岡山医学会雑誌
Volume volume120
Issue issue3
Content Type Journal Article
Author Oka, Takahiko| Tomoda, Jun| Kobashi, Haruhiko| Sakai, Nobuyuki| Sakaguchi, Kohsaku| Yamamoto, Kazuhide| Higashi, Toshihiro| Ito, Toshio| Yamada, Gotaro| Tsuji, Takao|
Published Date 1994
Publication Title 岡山医学会雑誌
Volume volume106
Issue issue5-6
Content Type Journal Article
Author Fujikawa, Tatsuya| Shiraha, Hidenori| Yamamoto, Kazuhide|
Published Date 2009-04-01
Publication Title 岡山医学会雑誌
Volume volume121
Issue issue1
Content Type Journal Article
Author Fukuda, Tetsuya| Yamada, Gotaro| Ogawa, Hiromichi| Okushin, Hiroaki| Hyodo, Ichinosuke| Nishihara, Takashi| Mizuno, Motowo| Sakamoto, Yuji| Nagashima, Hideo| Yamamoto, Kazuhide| Kobayashi, Toshinari| Yoshida, Tomoro|
Published Date 1984-04-30
Publication Title 岡山医学会雑誌
Volume volume96
Issue issue3-4
Content Type Journal Article
Author Hirao, Ken| Kawamoto, Hirofumi| Yamamoto, Kazuhide|
Published Date 2009-12-01
Publication Title 岡山医学会雑誌
Volume volume121
Issue issue3
Content Type Journal Article
Author Ogawa, Tsuneyoshi| Kawamoto, Hirofumi| Yamamoto, Kazuhide|
Published Date 2009-12-01
Publication Title 岡山医学会雑誌
Volume volume121
Issue issue3
Content Type Journal Article
JaLCDOI 10.18926/AMO/30943
FullText URL fulltext.pdf
Author Miyake, Yasuhiro| Yamamoto, Kazuhide|
Abstract <p>Autoimmune hepatitis (AIH) is a chronic and progressive disease characterized by histological interface hepatitis, hypergammaglobulinemia, and circulating autoantibodies. Multiple factors, including molecular mimicry, a genetic background including major histocompatibility complex class II, and defective function of regulatory T-cells, are involved in the pathogenesis. The diagnosis is made based on the scoring system of the International Autoimmune Hepatitis Group, the sensitivity and specificity of which are90%, respectively. AIH is classified into 3 sub-types based on the profiles of circulating autoantibodies: anti-nuclear antibody and/or smooth muscle antibody-positive (type 1), anti-liver-kidney microsomal antibody-positive (type 2), and anti-soluble liver antigen/liver-pancreas antigen antibody- positive (type 3). Recently, however, the number of atypical cases lacking the usual features has increased-for example, patients with acute-onset or fulminant-type AIH, autoantibody-negative patients, male patients, and patients with bile duct injury-and thus the clinical features of AIH have been diversified. AIH is responsive to immunosuppressive treatment in most cases; however, relapse occurs in more than 80% of patients within 1 year after immunosuppressive treatment withdrawal. The 10-year survival rate and the 10-year hepatocellular carcinoma-free rate are90%, respectively, indicating that some patients reach liver failure or develop hepatocellular carcinoma. To improve the prognosis of these patients, persistent normalization of transaminase is required.</p>
Keywords autoimmune hepatitis epidemiology pathogenesis diagnosis prognosis
Amo Type Review
Published Date 2008-08
Publication Title Acta Medica Okayama
Volume volume62
Issue issue4
Publisher Okayama University Medical School
Start Page 217
End Page 226
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18766204
Web of Science KeyUT 000258680900001
JaLCDOI 10.18926/AMO/30997
FullText URL fulltext.pdf
Author Yamamoto, Kazuhide| Makino, Yasuhiro| Itoshima, Tatsuya| Kobayashi, Toshinari| Tsuji, Takao|
Abstract <p>Phalloidin, a toxin from the plant Amanita phalloides, irreversibly polymerizes actin filaments and causes cholestasis. Three-dimensional structural changes induced by phalloidin in the bile canaliculi and the intra-acinar localization of these changes were studied in the rat liver by scanning and transmission electron microscopy. After 3 days of treatment, canalicular changes appeared mainly in zones 2 and 3 of Rappaport's acinus, but after 7 days of treatment changes occurred in bile canaliculi of the whole acinus. The changes in the bile canaliculi included tortuosity, saccular dilatation, loss of microvilli, bleb formation and elongation of canalicular side branches. Some side branches extended near to Disse's space, leaving only a thin cytoplasmic rim between the canalicular lumen and Disse's space. Kupffer cells were occasionally situated near such extended bile canaliculi and protruded their processes into the hepatic cord. These results suggest that bile canaliculi in zone 3 are more susceptible to phalloidin toxicity than those in zone 1 and that biliary constituents may leak from such altered bile canaliculi.</p>
Keywords phalloidin bile canaliculi choletasis
Amo Type Article
Published Date 1988-08
Publication Title Acta Medica Okayama
Volume volume42
Issue issue4
Publisher Okayama University Medical School
Start Page 207
End Page 213
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3177006
Web of Science KeyUT A1988P884600004
JaLCDOI 10.18926/AMO/31029
FullText URL fulltext.pdf
Author Makino, Yasuhiro| Yamamoto, Kazuhide| Tsuji, Takao|
Abstract <p>The three-dimensional arrangement of ductular structures formed by oval cells in rats fed 2-acetylaminofluorene (2-AAF) was studied by scanning electron microscopy (SEM) of biliary tract casts and light microscopy of sections of liver injected with india ink via the biliary tract. Both resin and india ink were well injected up to bile ductules, and the findings of each method correlated with each other. By the second week after 2-AAF administration, a few oval cells appeared in the periportal areas forming ductular structures which connected with the portal bile ducts. At the 4th week, increased ductular structures occupied two thirds of the lobule and formed networks communicating with each other, and with the portal bile ducts. At the 8th week, such ductular structures were compressed around hyperplastic nodules and appeared like a basket in biliary casts examined by SEM. Although a histochemical study of gamma-glutamyl transpeptidase revealed activity both on the luminal side of the ductular structures and hepatocytes in hyperplastic nodules, no transition was observed between these two cell populations. These results suggest that oval cells have characteristics more similar to those of biliary epithelia than of hepatocytes, and have no relation to the development of hyperplastic nodules.</p>
Keywords oval cells biliary tract casts scanning electron microscopy hyperplastic nodules hepatocarcinogenesis
Amo Type Article
Published Date 1988-06
Publication Title Acta Medica Okayama
Volume volume42
Issue issue3
Publisher Okayama University Medical School
Start Page 143
End Page 150
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2899946
Web of Science KeyUT A1988P034000004
JaLCDOI 10.18926/AMO/31826
FullText URL fulltext.pdf
Author Fujikawa, Tatsuya| Shiraha, Hidenori| Yamamoto, Kazuhide|
Abstract <p>Serum des-gamma-carboxy prothrombin (DCP) is commonly used to detect hepatocellular carcinoma (HCC). This review focuses on the clinical features of DCP-positive HCC and the molecular function of DCP in HCC. DCP-positive HCC demonstrates more aggressive clinicopathological features than DCP-negative HCC. Analysis of the biological effects of DCP revealed that DCP acts as a growth factor in both an autocrine and paracrine manner. DCP stimulates HCC cell proliferation through the Met-Janus kinase 1-signal transducer and activator of transcription 3 signaling pathway, whereas for vascular endothelial cells, it stimulates cell proliferation and migration through the kinase insert domain receptor-phospholipase C-gamma-mitogen-activated protein kinase signaling pathway.</p>
Keywords des-gamma-carboxy prothrombin hepatocellular carcinoma signaling pathway cell proliferation angiogenesis
Amo Type Review
Published Date 2009-12
Publication Title Acta Medica Okayama
Volume volume63
Issue issue6
Publisher Okayama University Medical School
Start Page 299
End Page 304
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20035286
Web of Science KeyUT 000273145900001
Author Nishimura, Mamoru| Takaki, Akinobu| Tamaki, Naofumi| Maruyama, Takayuki| Onishi, Hideki| Kobayashi, Sayo| Nouso, Kazuhiro| Yasunaka, Tetsuya| Koike, Kazuko| Hagihara, Hiroaki| Kuwaki, Kenji| Nakamura, Shinichiro| Ikeda, Fusao| Iwasaki, Yoshiaki| Tomofuji, Takaaki| Morita, Manabu| Yamamoto, Kazuhide|
Published Date 2013-01-30
Publication Title Hepatology Research
Volume volume43
Issue issue10
Content Type Journal Article
Author Fujii, Masakuni| Kawamoto, Hirofumi| Yamamoto, Kazuhide|
Published Date 2010-12-01
Publication Title 岡山医学会雑誌
Volume volume122
Issue issue3
Content Type Journal Article
Author Hiraoka, Sakiko| Kato, Jun| Fujiki, Shigeatsu| Kaji, Eisuke| Morikawa, Tamiya| Murakami, Takatoshi| Nawa, Toru| Kuriyama, Motoaki| Uraoka, Toshio| Ohara, Nobuya| Yamamoto, Kazuhide|
Published Date 2011-08-01
Publication Title 岡山医学会雑誌
Volume volume123
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/48568
FullText URL 66_3_279.pdf
Author Nishimura, Mamoru| Nouso, Kazuhiro| Kariyama, Kazuya| Wakuta, Akiko| Kishida, Masayuki| Wada, Nozomu| Higashi, Toshihiro| Yamamoto, Kazuhide|
Abstract The artificial ascites technique is often used during radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC) treatment because it prevents visceral damage and improves visualization by minimizing interference of the lungs and mesentery. This study determined the efficacy and safety of RFA using the artificial ascites technique in HCC patients. We examined 188 HCC patients who were treated by RFA and fulfilled the Milan criteria. Treatment outcomes (complete ablation rate, local recurrence rate, complication rate, liver function including total bilirubin level, alanine aminotransferase level, albumin level, and prothrombin time) were compared among patients divided into 3 groups based on the volume of artificial ascites injected:GroupⅠ (n=86), no artificial ascites injected;GroupⅡ (n=35), <1,000ml artificial ascites injected;and Group Ⅲ (n=67), >1,000ml artificial ascites injected. No significant difference was observed in complete ablation or local recurrence rates among the 3 groups, or in the extent of liver function damage after RFA. Artificial ascites disappeared within 7 days; additional diuretics were needed only in 5 (all from Group Ⅲ) of 102 patients. No serious complications such as intestinal perforation or intraperitoneal bleeding were observed. Thus, we found that artificial ascites injection during RFA is effective and safe, and can be used to prevent major procedural complications.
Keywords radiofrequency ablation hepatocellular carcinoma artificial ascites
Amo Type Original Article
Published Date 2012-06
Publication Title Acta Medica Okayama
Volume volume66
Issue issue3
Publisher Okayama University Medical School
Start Page 279
End Page 284
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22729109
Web of Science KeyUT 000305669700012
JaLCDOI 10.18926/AMO/53120
FullText URL 69_1_37.pdf
Author Iwamuro, Masaya| Okada, Hiroyuki| Takata, Katsuyoshi| Kawai, Yoshinari| Kawano, Seiji| Nasu, Junichiro| Kawahara, Yoshiro| Tanaka, Takehiro| Yoshino, Tadashi| Yamamoto, Kazuhide|
Abstract The sensitivity and specificity of magnified endoscopic features for differentiating follicular lymphoma from other diseases with duodenal whitish lesions have never been investigated. Here we compared the magnified endoscopic features of duodenal follicular lymphoma with those of other whitish lesions. We retrospectively reviewed the cases of patients with follicular lymphoma (n=9), lymphangiectasia (n=7), adenoma (n=10), duodenitis (n=4), erosion (n=1), lymphangioma (n=1), and hyperplastic polyp (n=1). The magnified features of the nine follicular lymphomas included enlarged villi (n=8), dilated microvessels (n=5), and opaque white spots of various sizes (n=9). The lymphangiectasias showed enlarged villi, dilated microvessels, and white spots, but the sizes of the white spots were relatively homogeneous and their margin was clear. Observation of the adenoma and duodenitis revealed only whitish villi. Although the lymphangioma was indistinguishable from the follicular lymphomas by magnified features, it was easily diagnosed based on the macroscopic morphology. In conclusion, magnified endoscopic features, in combination with macroscopic features, are useful for differentiating follicular lymphomas from other duodenal diseases presenting whitish lesions.
Keywords duodenal neoplasm follicular lymphoma gastrointestinal lymphoma magnifying endoscopy
Amo Type Original Article
Published Date 2015-02
Publication Title Acta Medica Okayama
Volume volume69
Issue issue1
Publisher Okayama University Medical School
Start Page 37
End Page 44
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 25703169
Web of Science KeyUT 000349740300004
JaLCDOI 10.18926/AMO/49667
FullText URL 67_2_93.pdf
Author Kita, Masahide| Yokota, Kenji| Okada, Hiroyuki| Take, Susumu| Takenaka, Ryuta| Kawahara, Yoshiro| Oguma, Keiji| Matsushita, Osamu| Yamamoto, Kazuhide|
Abstract Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer and duodenal ulcer accompanied by atrophic gastritis in background mucosa. H. pylori strains were stored at -80℃ prior to the experiments being carried out. We analyzed iceA, babA, vacA, cagA, and cagE genes by PCR. The cagA gene was analyzed through sequencing of the C-terminal region containing the EPIYA motif, which is related to tyrosine phosphorylation. Severe atrophy was observed in patients with gastric ulcer. The major phenotype of the vacA gene was s1c/m1 (93オ). The cagA gene was detected in all strains. The cagE gene was not detected in 2 and 5 strains from the mild cases and severe cases, respectively. The major cagA EPIYA motif, which is amino acids repeat in the C terminus, was the A-B-D type (44 of 58 strains). The virulence genes were not statistically associated with the severity of atrophy in the background gastric mucosa in humans. Not only identification of bacterial virulence factors but also studies of the host response will be necessary to investigate the progression of gastric atrophy and subsequent cancer development in humans.
Keywords Helicobacter pylori virulence genes chronic atrophic gastritis
Amo Type Original Article
Published Date 2013-04
Publication Title Acta Medica Okayama
Volume volume67
Issue issue2
Publisher Okayama University Medical School
Start Page 93
End Page 98
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 23603925
Web of Science KeyUT 000317801700003
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/52508