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ID 17137
Eprint ID
17137
FullText URL
Thumnail 89_667.pdf 1.17 MB
Title Alternative
A new rapid assay method of collagenase activity and its clinical applications Part 1. A rapid assay method of collagenase activity using (14)C-labeled soluble collagen as substrate
Author
Terato, Kuniaki
Abstract
A rapid assay method of collagenase activity has been developed using (14)C-labeled soluble collagen as substrate. This method is based on following three parts; first, carrying out the enzyme reaction with (14)C-labeled collagen in solution containing 0.25M glucose to prevent collagen fiblil formation at 35℃ under neutral condition, second, denaturation of the enzyme digestion products at 35℃ for 1hr after stopping the reaction by adding o-phenanthroline and third, selective extraction of the denatured (14)C-products into dioxane at a final concentration of 50 % and counting (14)C-activity in the surpernatant. The rate of the reaction was about 10 times higher than that obtained by the conventional method using reconstituted collagen fiblils as substrate and relationship between enzyme activity and enzyme concentration was linear over a wider range regardless of the purity of collagenase.
Published Date
1977-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume89
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
667
End Page
677
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/89/5-6/89_5-6_667/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma