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ID 16274
Eprint ID
16274
FullText URL
Thumnail 98_457.pdf 425 KB
Title Alternative
Helix pomatia A hemagglutinin binding activity to peripheral T-lymphocytes in malignant digestive tract diseases Part 1: Assay method based on (125)I-labelled Helix pomatia A hemagglutinin binding to peripheral lymphocytes
Author
Yasuhara, Takashi
Abstract
For studing aberrant cellular immunity in malignant digestive tract diseases, Helix pomatia A hemagglutinin (HP) binding activity, a T cell surface marker, to peripheral lymphocytes was studied in patients with digestive tract cancer. In this paper, basic experiments on an assay method based on (125)I-labelled HP binding to neuraminidase-treated peripheral lymphocytes are described. HP was labelled with (125)I using the chlormine-T method, and the purif-ication of (125)I-labelled HP was achieved by agarose-aminocaproyl-D-galactosamine affinity chromatography. The results show that (125)I-labelled HP could specifically bind to neuraminidase-treated lymphocytes as cold HP. The optimum assay condition was determined to be: 1×10(6) neuraminidase-treated peripheral lymphocytes incubated with 0.4μg (125)I-labelled HP in 0.5 ml of reaction mixture at 25℃ for 90 min with gentle shaking. In addition, the effect of treatment of peripheral lymphocytes with neuraminidase on E-rosette formation was studied. Enhancement of E-rosette formation by neuraminidase-treatment was not statistically significant.
Keywords
Helix pomatia A hemagglutinin
結合活性
消化器癌
末梢血リンパ球
neuraminidase
Published Date
1986-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume98
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
457
End Page
464
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/98/5-6/98_5-6_457/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma