Author Oku, Hachiro|
Published Date 1993
Publication Title 岡山大学農学部学術報告
Volume volume81
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative エンドウプロトプラスト細胞における植物プロモータの発現
FullText URL 080_0051_0060.pdf
Author Yamada, Tetsuji| Hashimoto, Tadaaki| Sriprasertsak, Permpong| Kato, Hisaharu| Ichinose, Yuki| Shiraishi, Tomonori| Oku, Hachiro|
Abstract High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor.
Publication Title 岡山大学農学部学術報告
Published Date 1992
Volume volume80
Issue issue1
Start Page 51
End Page 60
ISSN 0474-0254
language 英語
File Version publisher
Title Alternative エンドウ褐紋病菌の胞子発芽液に含まれるピサチン蓄積誘導物質の分離と性質
FullText URL 059_0001_0009.pdf
Author Thanutong, Porntip| Oku, Hachiro| Shiraishi, Tomonori| Ouchi, Seiji|
Abstract An elicitor of pisatin accumulation was isolated and partially characterized from spore germination fluid, mycelial extract, and cell wall preparation of a pea pathogen, Mycosphaerella pinodes. The elicitor was found to be a polysaccharide or glycoprotein and the active component was composed of glucose. The approximate molecular weight was 70,000 daltons. This elicitor elicited pisatin accumulation in pea leaves at a concentration of 10 ug/ml and the maximum activity was ca. 350-400 ug/ml, but did not elicit phytoalexins in soybean, bean, and red clover. High molecular weight preparations from the mycelial extract and mycelial wall of Mycosphaerella melonis and Stemphylium sarcinaeforme, nonpathogens of pea, elicited pisatin accumulation in pea leaves.
Publication Title 岡山大学農学部学術報告
Published Date 1982
Volume volume59
Issue issue1
Start Page 1
End Page 9
ISSN 0474-0254
language 英語
File Version publisher
Title Alternative 殺菌剤、ベノミル、チウラムの土壌微生物相と2~3の土壌生息性糸状菌に及ぼす影響
FullText URL 054_0001_0008.pdf
Author Oku, Hachiro| Oki, Kazuo| Shiraishi, Tomonori| Sato, Kenji| Ouchi, Seiji|
Abstract Effects of Benomyl and Thiram on the soil microflora and some soil inhabitant fungi were studied using the_ soil (sandy loam) of the experimental field, Okayama University. Under field conditions, heavy application of Benomyl did not affect significantly the soil microflora. Thiram, however, reduced the fungal population in soil to 1/6 at the next day of treatment, but recovered to the normal level after 6 days. Under laboratory conditions, both fungicides did not affect soil microflora. Population of Benomyl-to lerant fungi was about 1/10 of the total fungi and increased slightly in the field soil by treatment with Benomyl at the later stage of experiment during June to October. Neither Thiram-tolerant fungus nor bacterium was found in both Thiram-treated and non-treated soils. A fungus highly tolerant to Benomyl was isolated and identified as Aspergillus versicolor, and found to not have the metabolic activity to degrade BCM. The absorption of BCM by the mycelia of this tolerant fungus, A. versicolor, was less than half of the BCM-sensitive one, such as Cladosporium harbarum.
Publication Title 岡山大学農学部学術報告
Published Date 1979
Volume volume54
Issue issue1
Start Page 1
End Page 8
ISSN 0474-0254
language 英語
File Version publisher
Title Alternative Control Measure of Brown Spot of Grapes
FullText URL 049_0001_0006.pdf
Author Oku, Hachiro| Hatamoto, Motomu| Ouchi, Seiji| Shiraishi, Tomonori| Tateishi, Michihiro| Fujii, Shintaro|
Abstract ブドウ褐点病防除のための農薬のスクリーニングを行なうとともに,発生生態の観察結果を含めて本病の防除施策について論じた. 供用農薬のなかでは,ベンレート,サニパー,DF125が有効であった. 自然発生,特に汚果の防止には硫酸ニコチンの混用が有効であった. 外気の直接導入を避けるような温室出入口の構造改善,室内浄化,誘枝線の更新,過湿の回避などが有効な技術的な防除策と考えられた。
Publication Title 岡山大学農学部学術報告
Published Date 1977
Volume volume49
Issue issue1
Start Page 1
End Page 6
ISSN 0474-0254
language 日本語
File Version publisher
Title Alternative クラドスポリウム属菌によるブドウの褐点病について
FullText URL 048_0017_0022.pdf
Author Ouchi, Seiji| Hatamoto, Motomu| Oku, Hachiro| Shiraishi, Tomonori| Yokoyama, Tatsuo| Tateishi, Michihiro| Fujii, Shintaro|
Abstract Cladosporium cladosporioides (Fresenius) de Vries and Cladosporium herbarum (Pers. ) Link ex Fr. were isolated from grape berries that had been commonly called 'black navel', and were found to cause the disease. In view of the colour of lesions that were observed most frequently at the stage of maturing, the disease was named brown spot of grapes.
Publication Title 岡山大学農学部学術報告
Published Date 1976
Volume volume48
Issue issue1
Start Page 17
End Page 22
ISSN 0474-0254
language 英語
File Version publisher
Title Alternative Chitin Component in Haustorial Wall of Powdery Mildew Fungus of Barley
FullText URL 047_0021_0024.pdf
Author Shiraishi, Tomonori| Ouchi, Seiji| Oku, Hachiro|
Abstract 純寄生性病害の感染成立に中心的役割を演ずると考えられている吸器の性質を明らかにするために,オオムギうどんこ病菌の吸器形成過程における吸器壁のキチン質の生成に関して組織化学的に検討した. その結果,吸器壁はキトーサン反応陽性で,菌糸壁と同様キチン質がその骨格をなしているものと考えられる. さらに,経時的な検討の結果,感染初期に形成される吸器原基において,すでにキチン反応が陽性であり,キチン合成系の活性化は,吸器機能発現のための一つの重要な過程であると推定される。
Publication Title 岡山大学農学部学術報告
Published Date 1976
Volume volume47
Issue issue1
Start Page 21
End Page 24
ISSN 0474-0254
language 日本語
File Version publisher
Author Ouchi, Seiji| Oku, Hachiro| Hibino, Chihaya|
Published Date 1976
Publication Title 岡山大学農学部学術報告
Volume volume47
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative 土壌菌による有機塩素殺菌剤、ダコニールの吸収と脱クロールについて
FullText URL 047_0007_0013.pdf
Author Oku, Hachiro| Shiraishi, Tomonori| Ouchi, Seiji| Horiuchi, Sayoko|
Abstract Fungi tolerant to an organochlorine fungicide, tetrachloroisophthalonitrile (TCPN), was selected and isolated from various soil samples, and examined for the activity to metabolize TCPN. The population of tolerant fungi was extremely large in the plastic house - soil on which TCPN has been used for six years to control diseases. This is probably due to the change of soil microflora owing to the decline of TCPN-sensitive competitors. Many tolerant fungi took up TCPN very rapidly from culture filtrate. Of ten tolerant isolates tested, two isolates, C-F- I and V-P-5, were found to metabolize TCPN. The metabolite was isolated in pure form by preparative TLC, and identified as trichloroisophthalonitrile by mass spectrometry. C-F-1 was identified as Aspergillus luchuensis and V-P-5 as Penicillium godlewskii. Namely, these soil fungi dechlorinate from tetrachloroisophthalontrile to trichloroisophthalonitrile.
Publication Title 岡山大学農学部学術報告
Published Date 1976
Volume volume47
Issue issue1
Start Page 7
End Page 13
ISSN 0474-0254
language 英語
File Version publisher
Author Oku, Hachiro| Hatamoto, Motomu| Ouchi, Seiji| Fujii, Shintaro|
Published Date 1975
Publication Title 岡山大学農学部学術報告
Volume volume45
Issue issue1
Content Type Departmental Bulletin Paper
Author Ouchi, Seiji| Oku, Hachiro| Yoshida, Haruo|
Published Date 1974
Publication Title 岡山大学農学部学術報告
Volume volume44
Issue issue1
Content Type Departmental Bulletin Paper
Author Oku, Hachiro| Ouchi, Seiji| Yoshida, Haruo|
Published Date 1974
Publication Title 岡山大学農学部学術報告
Volume volume44
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative 農業用殺菌剤、植物病原菌の有害代謝産物によるファイトアレキシンの生産
FullText URL 042_0017_0020.pdf
Author Oku, Hachiro| Nakanishi, Toshiro| Shiraishi, Tomonori| Ouchi, Seiji|
Abstract The endocarp of the fresh pea pod incubated with solution or suspension of agricultural fungicides, phytotoxic metabolites of plant pathogenic fungi formed the pea phytoalexin, pisatin. Among the compounds tested, cycloheximide, triazine, dichlone, phenyimercury acetate, UV degradation product of phenylmercury acetate, triphenyltin fungicides, and 3-hydroxy-5-methylisoxazole induced pisatin. Ophiobolin, a toxin from Cochliobolus mtyabeanus, and ascochitine, a toxic metabolite from Ascochyta fabae, also induced pisatin. The possibilities of the development of harmless plant disease control agents was discussed in relation- to the induced synthesis of phytoalexins.
Publication Title 岡山大学農学部学術報告
Published Date 1973
Volume volume42
Issue issue1
Start Page 17
End Page 20
ISSN 0474-0254
language 英語
File Version publisher