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ID 57458
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Author
Sumi, Tomonari Department of Chemistry, Faculty of Science, Okayama University ORCID Kakenhi
Imamura, Hiroshi Graduate School of Advanced integration Science, Chiba University
Morita, Takeshi Graduate School of Advanced integration Science, Chiba University
Nishikawa, Keiko Graduate School of Advanced integration Science, Chiba University
Abstract
A small-angle X-ray scattering has been used to probe protein–protein interaction in solution. Conventional methods need to input modeled potentials with variable/invariable parameters to reproduce the experimental structure factor. In the present study, a model-free method for extracting the excess part of effective interaction potential between protein molecules in solutions over an introduced hard-sphere potential by using experimental data of small-angle X-ray scattering is presented on the basis of liquid-state integral equation theory. The reliability of the model-free method is tested by the application to experimentally derived structure factors for dense lysozyme solutions with different solution conditions [Javid et al., Phys. Rev. Lett. 99, 028101 (2007), Schroer et al., Phys. Rev. Lett. 106, 178102 (2011)]. The structure factors calculated from the model-free method agree well with the experimental ones. The model-free method provides the following picture of the lysozyme solution: these are the stabilization of contact-pair configurations, large activation barrier against their formations, and screened Coulomb repulsion between the charged proteins. In addition, the model-free method will be useful to verify whether or not a model for colloidal system is acceptable to describing protein–protein interaction.
Keywords
Protein solutions
Lysozyme
Protein-protein interaction
Liquid state theory
Integral equation
DLVO model
Published Date
2014-04-01
Publication Title
Journal of Molecular Liquids
Volume
volume200
Issue
issuePart.A
Publisher
Elsevier Science
Start Page
42
End Page
46
ISSN
01677322
NCID
AA10636062
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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author
DOI
Web of Sience KeyUT
Related Url
isVersionOf https://doi.org/10.1016/j.molliq.2014.03.014
Citation
Tomonari Sumi, Hiroshi Imamura, Takeshi Morita, Keiko Nishikawa, A model-free method for extracting interaction potential between protein molecules using small-angle X-ray scattering, Journal of Molecular Liquids, Volume 200, Part A, 2014, Pages 42-46, ISSN 0167-7322, https://doi.org/10.1016/j.molliq.2014.03.014.
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
25610121 : Chemomechanical network modeling for molecular motor dynamics of kinesin