FullText URL JoAmChemSoc_2017_139_12_4376.pdf
Author Niho, Akiko| Yoshizawa, Susumu| Tsukamoto, Takashi| Kurihara, Marie| Tahara, Shinya| Nakajima, Yu| Mizuno, Misao| Kuramochi, Hikaru| Tahara, Tahei| Mizutani, Yasuhisa| Sudo, Yuki|
Abstract In organisms, ion transporters play essential roles in the generation and dissipation of ion gradients across cell membranes. Microbial rhodopsins selectively transport cognate ions using solar energy, in which the substrate ions identified to date have been confined to monovalent ions such as H+, Na+, and Cl-. Here we report a novel rhodopsin from the cyanobacterium Synechocystis sp. PCC 7509, which inwardly transports a polyatomic divalent sulfate ion, SO42-, with changes of its spectroscopic properties in both unphotolyzed and photolyzed states. Upon illumination, cells expressing the novel rhodopsin, named Synechocystis halorhodopsin (SyHR), showed alkalization of the medium only in the presence of Cl- or SO42-. That alkalization signal was enhanced by addition of a protonophore, indicating an inward transport of Cl- and SO42- with a subsequent secondary inward H+ movement across the membrane. The anion binding to SyHR was suggested by absorption spectral shifts from 542 to 536 nm for Cl- and from 542 to 556 nm for SO42-, and the affinities of Cl- and SO42- were estimated as 0.112 and 5.81 mM, respectively. We then performed time-resolved spectroscopic measurements ranging from femtosecond to millisecond time domains to elucidate the structure and structural changes of SyHR during the photoreaction. Based on the results, we propose a photocycle model for SyHR in the absence or presence of substrate ions with the timing of their uptake and release. Thus, we demonstrate SyHR as the first light-driven polyatomic divalent anion (SO42-) transporter and report its spectroscopic characteristics.
Note This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher.
Published Date 2017-03-29
Publication Title Journal of the American Chemical Society
Volume volume139
Issue issue12
Publisher American Chemical Society
Start Page 4376
End Page 4389
ISSN 00027863
NCID AA00692602
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 28257611
DOI 10.1021/jacs.6b12139
Web of Sience KeyUT 000398247100034
Related Url isVersionOf https://doi.org/10.1021/jacs.6b12139