Title Alternative Cytoskeletal elements in an acoelomorph worm, Praesagittifera naikaiensis
FullText URL poalas_034_021_180706.pdf
Author Ikeda, Risa| Fujiwara, Chiho| Hamada, Mayuko| Sakamoto, Tatsuya| Saito, Noboru| Ando, Motonori|
Abstract Acoel flatworms can move in a variety of ways such as muscular and ciliary movements via cytoskeletal elements and their neural regulations. However, those locomotive mechanisms have not yet been fully elucidated. In this study, we examined the distribution of cytoskeletal elements including filamentous actin (F-actin) and tubulin, and the neuroanatomical organization in an acoelomorph worm, Praesagittifera naikaiensis (P. naikaiensis). Video microscopy revealed the elongation/contraction and the bending/rotation processes, and the ciliary gliding movement of P. naikaiensis. Histochemical and morphological analysis demonstrated that F-actin networks of inner longitudinal and outer circular muscle fibers were positioned along the entire surface of the body, and that the average distance between the circular muscle fibers in the contracted organism was decreased in the anterior region compared with that in the elongated organism. Electron microscopy showed dense bodies on the muscle cells of P. naikaiensis, which indicates that those muscle cells have the appearance of vertebrate smooth muscle cells. Immunohistochemical analysis revealed that -tubulin-positive signals on the ciliary microtubules had close contact with the F-actin network, and that neurite bundles labelled with anti dSap47 antibody as a neuronal marker run along the anterior-posterior body axis. These results indicate that the well-organized cytoskeletal elements and their neural control systems are preserved in P. naikaiensis, and that their mechanisms involved in those regulation systems are similar to those vertebrate systems. Further studies are needed to clarify the physiological mechanisms underlying the muscular and ciliary movements in P. naikaiensis.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2018-04
Volume volume34
Start Page 21
End Page 27
language 日本語
File Version publisher
NAID 120006469118
FullText URL J_Comp_Neurol_525_7_1586.pdf
Author Tamura, Kei| Kobayashi, Yasuhisa| Hirooka, Asuka| Takanami, Keiko| Oti, Takumi| Jogahara, Takamichi| Oda, Sen-ichi| Sakamoto, Tatsuya| Sakamoto, Hirotaka|
Keywords RRID AB_2060157 RRID: AB_2571636 RRID: AB_626757 Suncus murinus (suncus) gastrin-releasing peptide male reproductive function sexual dimorphism spinal cord
Note This is the peer reviewed version of the following article: Tamura K. Kobayashi Y. Hirooka A. et al. Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus). J Comp Neurol. 2017;525:1601–1613. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. 2018年5月公開予定
Published Date 2017-05-01
Publication Title Journal of Comparative Neurology
Volume volume525
Issue issue7
Publisher Wistar Institute of Anatomy and Biology
Start Page 1586
End Page 1598
ISSN 0021-9967
NCID AA00695917
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 27804131
Web of Sience KeyUT 000397559700004
Related Url https://doi.org/10.1002/cne.24138