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ID 14127
Eprint ID
14127
FullText URL
Thumnail 105_589.pdf 1.3 MB
Title Alternative
Studies of human bone marrow stromal cells in the aged Part 1. Influence of aging on composition, growing dynamics and cytokine production in human bone marrow stromal cells
Author
Deguchi, Seigo
Abstract
Bone marrow stromal cells (BMSC) play essential roles in hemopoiesis. In the present study, bone marrow specimens obtained from 23 normal volunteers were cultured with a modified version of Dexter's long term bone marrow culture system to investigate the influences of aging on human BMSC. The composition of BMSC was analyzed by immunofluorescence. The percentage of monocyte/macrophages, adipocytes and T-cells were significantly increased in the aged. BMSC from the aged proliferated more rapidly than those from the young during the first week of culture in the growing dynamics study. Cytokine concentration in the conditioned medium was also measured in terms of BMSC function by radioimmunoasssay or enzyme-linked immunosorbent assay. The concentration of macrophage colony stimulating factor (M-CSF) on day 21 increased in the culture from the young, while interleukin-6(IL-6) concentration was higher on day 7 in the culture from the aged. These findings show no evidnece of BMSC defects in the aged. They show evidnece suggesting advanced function.
Keywords
Long-term bone marrow culture
Human marrow stromal cells
Aging
Growing dynamics
Cytokine production
Published Date
1993
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume105
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
589
End Page
600
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/105/5-6/105_5-6_589/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma