JaLCDOI 10.18926/AMO/48081
FullText URL 66_1_53.pdf
Author Nakamura, Keiichiro| Hongo, Atsushi| Kodama, Junichi| Hiramatsu, Yuji|
Abstract The purpose of this study was to evaluate prognostic factors for epithelial ovarian cancer. We found that the pretreatment values of maximum standardized uptake (SUVmax) of the primary tumor by positron emission tomography/computed tomography (PET/CT), tumor marker CA125 and C-reactive protein (CRP) were correlated with clinical characteristics and prognosis for such patients. The clinical parameters and prognoses and their correlations with SUVmax of primary tumor, CA125 and CRP were examined for 51 patients with primary ovarian cancer. The SUVmax of the primary tumor had a statistically significant association with stage (p=0.010) and histology (p=0.001). CA125 was significant associated with stage (p=0.011), histology (p=0.005) and lymph node metastasis (p=0.025). CRP was also significantly associated with stage (p=0.049). Disease-free survival rates of patients exhibiting a high SUVmax, CA125 and CRP were significantly lower than those exhibiting a low SUVmax, CA125 and CRP levels (p=0.008, 0.034, and 0.037, respectively). Furthermore, overall survival rates of patients exhibiting a high SUVmax were significantly lower than those exhibiting a low SUVmax (p=0.049).The high SUVmax of primary tumor is an important factor for identifying ovarian cancer patients with a predictor for poor prognosis.
Keywords ovarian cancer SUVmax of primary tumor CA125 C-reactive protein predictor for poor prognosis
Amo Type Original Article
Published Date 2012-02
Publication Title Acta Medica Okayama
Volume volume66
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 60
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22358139
Web of Sience KeyUT 000300800700007
JaLCDOI 10.18926/AMO/31723
FullText URL fulltext.pdf
Author Araki, Shinako| Miyagi, Yasunari| Kawanishi, Kunihiro| Yamamoto, Junko| Hongo, Atsushi| Kodama, Junichi| Yoshinouchi, Mitsuo| Kudo, Takafumi|
Abstract <p>The in vitro radiosensitizing effects of docetaxel have been reported, but the DNA damage caused by the irradiation after docetaxel exposure has not been investigated. In this study, the authors attempted to evaluate the radiosensitizing effects in terms of cell survival and DNA single-strand breaks in a human ovarian adenocarcinoma cell line (known as line BG-1) and a human cervical squamous cell carcinoma cell line (known as line SiHa). The cell lines were exposed to various concentrations of docetaxel (from 2.27 x 10(-3) to 2.27 microg/ml) to investigate the cytocidal effects by colony-formation assay. DNA single-strand breaks after exposure to 2.27 microg/ml of docetaxel for 30 min or 100 min were measured by the alkaline-elution assay. The remarkable cytotoxicity of docetaxel followed by irradiation was observed when concentrations were greater than 2.27 x 10(-2) microg/ml in both cell lines. The combination of docetaxel and irradiation appears to be supraadditive. The DNA single-strand breaks induced by the irradiation were enhanced in both cell lines (BG-1; P &#60; 0.01, SiHa; P &#60; 0.05). The synergistic cytocidal effect cannot be explained quantitatively only by the single-strand breaks. </p>
Keywords docetaxel DNA single-strand break radiosensitizer
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 13
End Page 18
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873939
Web of Sience KeyUT 000174031300003
JaLCDOI 10.18926/AMO/31635
FullText URL fulltext.pdf
Author Yamamoto, Junko| Miyagi, Yasunari| Kawanishi, Kunihiro| Yamada, Shinako| Miyagi, Yuji| Kodama, Junichi| Yoshinouchi, Mitsuo| Kudo, Takafumi|
Abstract <p>The pharmacodynamic effects of cisdiamminedichloroplatinum(II) (CDDP) in vitro have been reported, but the dosage and exposure time in vitro have not been based on clinical observations of the drug's actions in vivo. In this study, the authors attempted to evaluate the pharmacodynamic effects of CDDP in vitro in terms of cell survival and DNA crosslinking by simulating unbound CDDP administration at varying concentrations to a rat mammary adenocarcinoma line (known as line 66). CDDP exposure was conducted by both constant concentration procedures and a simulated in vivo procedure. Colony formation assay for the surviving fraction and alkaline elution assay for DNA crosslink measurement were performed in order to evaluate the pharmacodynamics of CDDP. Cell survival was a function of the area under the drug concentration time curve (AUC) of unbound CDDP (R2 = 0.77, P &#60; 0.002) for all drug exposure procedures as analyzed by the analysis of covariance test. There was a strong correlation between the surviving fraction and the crosslink index of the total amount of DNA crosslinks (R2 = 0.85, P &#60; 0.0005). Both the total amount of DNA-DNA crosslinks and the DNA-protein crosslinks, of which the latter were dominant, were affected not by the exposure procedures, but by the AUC value (P &#60; 0.002). The thresholds of cytocidal effect were 1.59 mg.h/l for the AUC and 0.008 for the crosslink index. The pharmacodynamic effects in vitro by simulated in vivo exposure were identical to those of constant.</p>
Keywords pharmacodynamics pharmacokinetics simulation cisplatin crosslink
Amo Type Article
Published Date 1999-10
Publication Title Acta Medica Okayama
Volume volume53
Issue issue5
Publisher Okayama University Medical School
Start Page 201
End Page 208
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10561728
Web of Sience KeyUT 000083427100001
JaLCDOI 10.18926/AMO/31116
FullText URL fulltext.pdf
Author Kodama, Junichi| Hayase, Ryoji| Yoshinouchi, Mitsuo| Okuda, Hiroyuki| Kudo, Takafumi|
Abstract <p>P-glycoprotein is a transmembrane protein which acts as an energy-dependent drug efflux pump for a variety of anti-cancer drugs. The mdr-1 gene which encodes P-glycoprotein was successfully cloned in 1986. To investigate P-glycoprotein expression in diverse ovarian tumors, including benign, low malignant potential and malignant, immunohistochemical study was done using a monoclonal antibody (C 219). Overall, 8 out of the 59 epithelial ovarian tumors (13.6%) expressed P-glycoprotein. It was noted that 5 of the 12 mucinous tumors were found to express P-glycoprotein, while none of the 31 serous tumors were immunohistochemically positive. In 10 malignant ovarian tumors, P-glycoprotein immunostaining was examined both prior to and after chemotherapy. Nine of them did not express any P-glycoprotein before or after chemotherapy. However, one tumor expressed P-glycoprotein after six courses of multidrug resistance-related drug administration. These findings indicate that P-glycoprotein expression is not so common in ovarian tumors, regardless of their malignant potential. Nevertheless, the results suggest a strong association between P-glycoprotein expression and certain histological cell types in epithelial ovarian tumors. It is also possible that P-glycoprotein appears as a result of chemotherapy, but such a phenomenon can not occur unless chemotherapy is administered at high doses for a long period of time.</p>
Keywords P-glycoprotein epithelial ovarian tumor multidrug resistance immunohistochemistry
Amo Type Article
Published Date 1994-10
Publication Title Acta Medica Okayama
Volume volume48
Issue issue5
Publisher Okayama University Medical School
Start Page 249
End Page 255
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7863796
Web of Sience KeyUT A1994PP23600004
JaLCDOI 10.18926/AMO/30944
FullText URL fulltext.pdf
Author Kuramoto, Hiroyuki| Hongo, Atsushi| Liu, Yi-xuan| Ojima, Yojiro| Nakamura, Keiichiro| Seki, Noriko| Kodama, Junichi| Hiramatsu, Yuji|
Abstract <p>The insulin-like growth factor I receptor (IGF-IR) is exceptionally overexpressed in many cervicalcancer-derived cell lines. It is postulated that a decrease of p53 protein levels due to human papillomavirus (HPV) infection may contribute to the up-regulation of IGF-IR expression in cervical cancer cells because transcription of IGF-IR is strictly down-regulated by p53. To evaluate this fact in clinical cervical cancer specimens, we checked the expression levels and activated status of IGF-IR by immunohistochemistry. Formalin-fixed and paraffin-embedded specimens obtained by conization or hysterectomy were stained with anti-IGF-IR and with an antibody recognizing phosphorylated tyrosine at its c-terminus. The expression levels of IGF-IR were significantly high in cervical intraepithelial neoplasia (CIN) III and invasive cancer specimens. Phosphorylation of IGF-IR was promoted in all CIN and invasive cancer specimens, and its intensity was related to the promotion of lesions. Interestingly, IGF-IR overexpression was missing in the basal layer of CIN I and II lesions, whereas it was evenly distributed in CIN III and invasive cancer lesions. This IGF-IR overexpression pattern may be utilized in the diagnosis of HPV infection status in CIN lesions.</p>
Keywords insulin-like growth factor I receptor cervical cancer human papillomavirus tyrosil phosphorylation
Amo Type Original Article
Published Date 2008-08
Publication Title Acta Medica Okayama
Volume volume62
Issue issue4
Publisher Okayama University Medical School
Start Page 251
End Page 259
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18766208
Web of Sience KeyUT 000258680900005
Author Kodama, Junichi|
Published Date 2008-05-01
Publication Title 岡山医学会雑誌
Volume volume120
Issue issue1
Content Type Journal Article
Author 児玉 順一|
Published Date 1995-12-31
Publication Title
Content Type Thesis or Dissertation