Author Yanagi, Yoshinobu| Fujita, Mariko| Hisatomi, Miki| Matsuzaki, Hidenobu| Konouchi, Hironobu| Katase, Naoki| Nagatsuka, Hitoshi| Asaumi, Jun-ichi|
Published Date 201012
Publication Title Oral Radiology
Volume volume26
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/52009
FullText URL 67_6_359.pdf
Author Katashima, Kazunori| Kuroda, Masahiro| Ashida, Masakazu| Sasaki, Takanori| Taguchi, Takehito| Matsuzaki, Hidenobu| Murakami, Jun| Yanagi, Yoshinobu| Hisatomi, Miki| Hara, Marina| Kato, Hirokazu| Ohmura, Yuichi| Kobayashi, Tomoki| Kanazawa, Susumu| Harada, Sosuke| Takemoto, Mitsuhiro| Ohno, Seiichiro| Mimura, Seiichi| Asaumi, Junichi|
Abstract It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis.
Keywords ADC apoptosis necrosis hyperthermia cell density
Amo Type Original Article
Published Date 2013-12
Publication Title Acta Medica Okayama
Volume volume67
Issue issue6
Publisher Okayama University Medical School
Start Page 359
End Page 367
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 24356720
Web of Science KeyUT 000328915700004
Author Hisatomi, Miki|
Published Date 2008-03-25
Publication Title
Content Type Thesis or Dissertation
Author Hisatomi, Miki| Yanagi, Yoshinobu| Konouchi, Hironobu| Matsuzaki, Hidenobu| Takenobu, Toshihiko| Unetsubo, Teruhisa| Asaumi, Jun-ichi|
Published Date 2011-02
Publication Title Oral Oncology
Volume volume47
Issue issue2
Content Type Journal Article
Author Asaumi, Jun-ichi| Yanagi, Yoshinobu| Konouchi, Hironobu| Hisatomi, Miki| Matsuzaki, Hidenobu| Shigehara, Hiroshi| Kishi, Kanji|
Published Date 2004-9
Publication Title European Journal of Radiology
Volume volume51
Issue issue3
Content Type Journal Article
Author Asaumi, Jun-ichi| Yanagi, Yoshinobu| Konouchi, Hironobu| Hisatomi, Miki| Matsuzaki, Hidenobu| Kishi, Kanji|
Published Date 2004-7
Publication Title Oral Oncology
Volume volume40
Issue issue6
Content Type Journal Article