岡山大学農学部 Acta Medica Okayama 0474-0254 92 1 2003 Possible Involvement of AAAG Motif and PsDof1 in　Elicitor-Induced Gene Expression in Pea 21 26 EN Hikaru Seki Mizuri Marutani Yoshishige Inagaki Kazuhiro Toyoda Tomonori Shiraishi Yuki Ichinose Recently, we, isolated cDNA clone, PsDof1, from clicitor-treated pea cDNA library. The putative gene product, a PsDof1, encodes DNA binding protein that specifically binds the DNA fragment containing AAAG core sequence. In this paper we report that GST-PsDof1 fusion protein specifically binds to the promoter region containing AAAG core sequence(s) of PsCHS1, one of the elicitor-inducible genes encoding chalcone synthase (CHS). Furthermore the addition of DNA fragment containing AAAG motif to the 35S minimal promoter provided the elicitor-responsibility in transient transfection assay using pea protoplasts. These results suggest that PsDof1 might be involved in defense responses by acitivating the transcription by a binding to AAAG core sequence in the promoter of the defense-related genes in pea. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 植物病原細菌Pseudomonas syringae pv. tabaci の病原性機構の解析 EN Mizuri Marutani No potential conflict of interest relevant to this article was reported.