JaLCDOI 10.18926/15275
Title Alternative Cutaneous sarcoidosis : Clinical features and management
FullText URL 009_1_049_057.pdf
Author Nakata, Yasunari| Kataoka, Mikio| Hiramatsu, Junichi| Okazaki, Kazunori| Harada, Mine|
Abstract 1976年から1996年に岡山大学第2内科を受診したサルコイドーシス255例のうち,65例(71病変)の皮膚サルコイドーシス(皮膚サ症)について,臨床経過,臨床検査成績,胸部病変との関連,予後について検討した。皮膚サ症患者の年齢は18歳から77歳で中央値は51歳であった。女性例が42例(65%)と多 く,特に50歳代女性に43%と最も高率であった。皮膚病型では結節型33例,皮下型16例,び慢浸潤型3例,局面型6例,結節性紅斑様皮疹3例,苔癬様型1例,瘢痕浸潤9例であった。皮膚サ症では非皮膚サ症に比して気管支肺胞洗浄液中リンパ球の低率が見られたが,その他の臨床成績に差は認められなかった。皮膚病型別に検討すると,局面型,び慢浸潤型では気管支肺胞洗浄液中リンパ球CD4/CD8比は高く,3年後の皮膚および肺病変の残存率は高かった。一方皮下型ではCD4/CD8比は低く皮膚,肺病変の残存率も低かった。
Abstract Alternative Of 255 patients with clinical and histologic evidence of sarcoidosis, 65 (25percent) presented with various cutaneous manifestations of the disease. Cutaneous sarcoid patients were 42 women and 23 men ranging from 18 to 77 years in age. The skin lesions have included nodular infiltration in 33 patients, subcutaneous tumor in 16 patients, lupus pernio in 3, annual plaques in 6, erythema nodosum-like eruption in 3, lichinoid in 1, and scars in 9. There was no correlation between the presence of cutaneous lesions and chest radiolographic stages, abnormal pulmonary gallium uptake, cell differenciations of bronchoalveolar lavages and serum angiotensin converting enzyme activities. Forty-six patients followed for at least 3 years to determine the course of the disease. Subcutaneous tumors tended to heal within 3 years, while lupus pernio and plaque lesions were likely to have a protracted course.
Keywords サルコイドーシス (sarcoidosis) 皮膚 (skin) 臨床経過 (clinical-features) 予後 (prognosis)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1998-09-30
Volume volume9
Issue issue1
Start Page 49
End Page 57
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002307751
JaLCDOI 10.18926/11760
Title Alternative Alteration of thermostable phosphatase activity after hydrophobic chromatography
FullText URL 007_1_017_021.pdf
Author Mori, Shuji| Okamoto, Motoi| Nakata, Yasunari| Endo, Hiroshi|
Abstract 耐熱性ホスファターゼを含んだBacillus stearothermophilus 粗酵素試料を、リソースIsoによる疎水性クロマトグラフィにかけ分離を行った。1.5M→0M 硫酸アンモニウムの直線逆濃度勾配によって溶出を行ったところ、ホスファターゼは不活性な形で溶出され、これは硫酸アンモニウムによる濃度依存的阻害に起因することが判明した。ホスタファーゼの反応混合液に種々の濃度の硫酸アンモニウムを添加したところ、0.15Mの硫酸アンモニウム存在下で約80%の阻害が認められた。加えて、この阻害作用は単に硫酸アンモニウムの添加によってpHが酸性側に傾くことによるものではないことも明らかとなった。
Abstract Alternative Thermostable phosphatase partially purified from thermophilic bacteria, Bacillus stearothermophilus, was chromatographed on Resource Iso hydrophobic resin. When linear reverse gradient elution with 1.5 M → 0 M ( NH(4))(2)SO(4) was performed, phosphatase was found to be eluted as latent form, which revealed dose-dependent inhibitory effect of (NH(4))(2)SO(4) on phosphatase. When various concentrations of (NH(4))(2)SO(4) were added into phosphatase reaction mixture, about 80% inhibition was observed in the presence of 0.15 M (NH(4))(2)SO(4). Acidification by adding (NH(4))(2)SO(4) was not responsible for this inhibition, because addition of (NH(4))(2)SO(4) solution which pH was previously adjusted to 9.0 showed same inhibitory effect.
Keywords 耐熱性ホスファターゼ (thermophilic phosphatase) 疎水性クロマトグラフィ (hydrophobic chromatography) 硫酸アンモニウム (ammonium sulfate) 濃度依存的阻害 (dose- dependent inhibition)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-08-30
Volume volume7
Issue issue1
Start Page 17
End Page 21
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002314041
JaLCDOI 10.18926/11873
Title Alternative Clearance study of polyethylene glycol-modified cholesterol esterase.
FullText URL 004_053_055.pdf
Author Mori, Shuji| Nakata, Yasunari| Endo, Hiroshi|
Abstract The clearance of polyethylene glycol-modified cholesterol esterase was investigated spectrophotometrically, as compared with native one. PEG-modification provided the significant elongation in clearance time of cholesterol esterase after intravenous injection in mouse. This method was shown to be effective for creating new biomaterials.
Keywords cholesterol esterase polyethylene glycol modification in vivo clearance
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1994-01-31
Volume volume4
Start Page 53
End Page 55
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313810
JaLCDOI 10.18926/11871
Title Alternative IgG Heavy-Chain Subclass Typing of Human Serum M-Protein -Optimal condition for the separation and the detection with high resolution of agarose gel isoelectrofocusing-
FullText URL 004_047_051.pdf
Author Ichimura, Mitsuko| Tohge, Hiroko| Sakiyama, Junko| Nakata, Yasunari| Endo, Hiroshi|
Abstract IgG Heavy-Chain subclass typing of human serum M-protein were isoelectrofocussed (IEF) in agarose gel, and then the bands of IgG were detected with peroxidase-conjugated anti-human IgG1-4 monoclonal antibodies on the same isoelectrofocussed agarose gel plate. This IEF enzyme immunodetection patterns were composed of four to eight discrete bands (The range of pI was 6.0 to 9.0). These bands were dependent on immunofixationbands. It was very specific and clear to detect the subclass of IgG Type M-protein in human serum using this method.
Keywords IgG Subclass M-Protein Isoelectrofocusing Monoclonal Antibodies
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1994-01-31
Volume volume4
Start Page 47
End Page 51
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313808
JaLCDOI 10.18926/11822
Title Alternative Sarcoidosis and Propionibacterium acnes
FullText URL 003_019_036.pdf
Author Nakata, Yasunari| Kataoka, Mikio| Kimura, Ikuro|
Abstract Although there have been numerous reports on the isolation of bacteria, fungus, and aetiological agents of chemical substances from specimens of patients with sarcoidosis, none of them have been substantiated. In any event, an understanding of the pathogenesis of pulmonary sarcoid is intimately linked to that of the processes involved in the accumulation of T-cells in the lower respiratory tract of individuals with sarcoidosis. Propionibacterium acnes was isolated at high rates and in high concetrations from lymph nodes in patients with sarcoidosis. However, the precise mechanism of granuloma formation and immunomodulation by P. acnes has not been elucidated yet. In patients with sarcoidosis, it was found that the high levels of interleukin-2 (IL-2) released from alveolar lymphocytes as well as interleukin-1, tumor necrosis factor (TNF) and interleukin-6 (IL-6) released from alveolar macrophages were stimulated by P. acnes. These cytokines (mainly IL-2), released by P. acnes in large quantities, play a major role in the compartmentalization of the T-cell population in the lung and lead to the formation of an alveolitis and granuloma in the lung parenchyma of patients with sarcoidosis. This paper focuses primarily on the role of the cytokine network in the pulmonary mononuclear cells in the lung of patients with sarcoidosis.
Keywords sarcoidosis macrophage T-cell interleukin-2 Propionibacterium acnes
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 19
End Page 36
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313339
JaLCDOI 10.18926/11763
Title Alternative Immunological Abnormalities of Alveolar Macrophages in Patients with Sarcoidosis
FullText URL 001_039_050.pdf
Author Nakata, Yasunari|
Abstract Bronchoalveolar lavage is an invaluable means of accurately evaluating the inflammatory and immune processes in the human lung. Sarcoidosis is a multisystem disorder characterized by heightened immune processes at sites of disease activity. The lung is most commonly involved. Although granulomas are charactaristic pathologic features of this disease, a number of studies suggest that the initial lesion in the lung is a T-cell alveolitis (an accumulation of T-cellls in the lung). There are a lot of findings that show abnormal functions of alveolar macrophages obtained by bronchoalveolar lavage in the release of various monokines and arachidonic acid metabolites and metabolize oxygen. In this review, the abnormalities of alveolar macrophages implicated in pulmonary T-cell alveolitis and fibrosis are reviewed and their potential roles in the lungs are discussed.
Abstract Alternative 間質性肺疾患の肺病変の検討は気管支肺胞洗浄法の開発により病巣よりの免疫担当細胞を容易に得られるようになり近年急速に発展した。一方では分子生物学の進歩はこれらの細胞の産生する各種サイトカイン、代謝産物の分離と、その作用機序を明らかにした。そしてサルコイドーシスの肺病巣の免疫担当細胞においても細胞表面抗原の発現異常、外来抗原の貪食・消化の異常、サイトカインの産生・放出の異常、活性酸素の産生・放出の異常、アラキドン酸代謝の異常時、多岐にわたって多くの成績が報告されている。本論文では免疫担当細胞のうち、特に肺マイクロファージの機能異常とサ症の初期病変と考えられる肺胞へのリンパ球の異常集積、alveolitis形成および胞隔の線維化との関与について検討をおこない、本症の発症機序について考察を加えた。
Keywords sarcoidosis alveolar macrophage monokine metabolize oxygen arachidonic acid metabolite
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 39
End Page 50
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313772
JaLCDOI 10.18926/11818
Title Alternative Alcoholic Liver Disease
FullText URL 003_001_012.pdf
Author Endo, Hiroshi| Nakata, Yasunari| Okamoto, Motoi| Mori, Shuji| Ohta, Takeo|
Abstract Relationship between ethanol drinking and organs injury was reviewed and special emphasis was put on alcoholic liver disease. Consumption of alcoholic beverage expressed as ethanol per capita of adult in Japan increased 2.1 times in these 25 years and it is still increasing. Although the incidence of alcoholic liver disease in Japan also increased greatly during the above period, it seems likely that plateau level is coming because of genetically defined, unique type of alcohol metabolism in Japanese. Sex differences in susceptibility to alcohol were discussed. Among the six types of alcoholic liver disease, alcoholic liver fibrosis is relatively frequent in Japan. Mechanism of liver injury has been studied extensively. Alcohol itself is toxic but other factors such as dietary fat are also important. Biochemical and immunological markers of drinking were presented. As for the treatment, most patients especially in early stages of the disease well respond to alcohol withdrawal, but therapy of alcohol dependence in the background of the disease is very difficult requiring cooperative works of different kinds of specialists.
Keywords alcohol liver epidemiology genetic factors alcohol dependence
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 1
End Page 12
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313726
JaLCDOI 10.18926/11917
Title Alternative Antibody to Propionibacterium acnes (P.acnes) in guinea pigs with pulmonary granulomatosis
FullText URL 005_065_070.pdf
Author Nakata, Yasunari| Mori, Yoshihiro| Ejiri, Tohgo| Kataoka, Mikio| Hiramatsu, Jun-ichi|
Abstract P.acnesを皮内前感作した後、P.acnes菌体壁成分をimcomplete Freund's adjuvantと共に気管内に投与して作製した実験的肺肉芽腫症モルモットにおける抗P.acnes抗体について検討した。気管支肺胞洗浄液中抗P.acnes抗体価は気管内投与後1週目ピークを示し2週目までは上昇が認められ、4週目では気管内投与前の値に復した。この経過は肺胞内リンパ球数の変動と軌を一にしており、抗体価とリンパ球数の間には正の強い相関が認められた。血清中の抗P.acnes抗体価は1週目に低下が認められ、2週以後に徐々に回復して4週以後は元に戻った。P.acnesの気管内投与により血清中の抗体は肺へと移行し、リンパ球浸潤による胞隔炎、更には類上皮細胞肉芽腫の形成にP.acnesの関与を示すものであった。
Abstract Alternative In guinia pig models of P.ances induced pulmonary granulomatosis, anti-P.acnes antibody activity in bronchoalveolar lavage fluid peaked at 1 week after the endotracheally injection and decreased progressively, attaining control animal value by 4 weeks. The change of lymphocyte counts paralleled the change of anti-P.acnes antibody activity levels in bronchoalveolar fluids. Furthermore, the degree of granuloma formation in the lung paralleled the rise of anti-P.acnes antibody activity level. This data suggests that the anti-P.acnes antibody play a central role in the induction of lymphocyte alveolitis in experimental pulmonary granulomatosis.
Keywords サルコイドーシス (sarcoidosis) プロピオニバクテリウム (propionibacteium acnes) 肺肉芽腫症 (pulmonary granulomatosis) 動物実験モデル (experimental model) 気管支肺胞洗浄液 (bronchoalveolar lavage)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1995-01-31
Volume volume5
Start Page 65
End Page 70
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313584
JaLCDOI 10.18926/15268
Title Alternative ヒト血清リポタンパク質とシクロデキストリンのアガロースゲル内における相互作用
FullText URL 007_2_093_097.pdf
Author Ichimura, Mitsuko| Tohge, Hiroko| Sakiyama, Junko| Mori, Shuji| Ito, Sekiko| Nakata, Yasunari| Endo, Hiroshi|
Abstract Cyclodextrins (CDs) are cyclic oligosaccharides with low molecular weight. They have been known to bind to some serum lipoproteins and to form complexes. To elucidate whether each serum lipoprotein subclass could be separated by electrophoresis using CDs, we performed preliminary experiment, in which lipoprotein-CDs interaction was examined on electrophoresis with agarose gel. When the supporting agarose gel containing both α-CD and β-CD was prepared and was applied to isoelectric focusing for fractionating serum lipoproteins, apoB lipoproteins were found to be clearly separated into several fractions on this electrophoresis. This finding suggested that apoB lipoprotein may be detected as isolated form by arranging amounts of added CDs.
Abstract Alternative シクロデキストリンは低分子量の環状オリゴ糖で, リポタンパク質と複合体を形成する。シクロデキストリンを使用した電気泳動による血清リポタンパク質サブクラスの分離の可能性を明らかにするために, リポタンパク質とシクロデキストリンとの泳動用ゲル内での相互作用について検討をおこなった。その結果,α-CDとβ-CDの2種類のCDをアガロースゲルに添加し,このゲルを泳動用支持体として等電点電気泳動をおこなったところ,apoB含有リポタンパク質が数分画分離された。したがって,ゲル内へのシクロデキストリンの適正な添加条件が設定できるならば,apoB含有リポタンパク質サブクラスの分離が可能となることが示唆された。
Keywords 血清apoB含有リポタンパク質 (serum apoB lipoprotein) シクロデキストリン (cyclodextrin) 等電点電気泳動 (isoelectric focusing (IEF)) agarose gel
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1997-01-31
Volume volume7
Issue issue2
Start Page 93
End Page 97
ISSN 0917-4494
language 英語
File Version publisher
NAID 120002307426
JaLCDOI 10.18926/15289
Title Alternative 変形性膝関節炎患者膝関節液中の細胞外マトリックス成分と可溶性VCAM-1
FullText URL 007_2_099_103.pdf
Author Tohge, Hiroko| Ichimura, Mitsuko| Sakiyama, Junko| Okamoto, Motoi| Nakata, Yasunari| Endo, Hiroshi|
Abstract It is generally accepted that the number of patients with osteoarthritis (OA) of the knee must increase in the future because of the increase of the aged. The pathogenic mechanisms of OA haven't been elucidated yet, but it is supposed that mediate pannus may cause the factors for irreversible cartilage damage in the inflammatory process. Therefore it is very important to find and predict OA at the early stage. Now there were many indices to determine joint damage with OA, for example, cytokines including IL, TNF, TGF, matrix metalloproteinase (MMPs), tissue inhibitor of metalloproteinase (TIMPs), proteoglycan (PG), hyaluronate (HA), super oxide, adhesion molecules including, ICAM-1, VCAM-1 and PECAM. In this study, we measured that the soluble VCAM-1 (sVCAM-1), soluble collagen (sCOLL), heparansulfate proteoglycan (HSPG), uronic acis and CRP in synovial fluids (SF) with OA. A positive correlation between the level of sVCAM-1 and sCOLL (ρ = 0.652) was noted. However there was no factor showing a correlation with CRP. It was suggested that the levels of sVCAM-1 and sCOLL in the synovia fluid were active inflammation indices of knee damage, as the different aspects from CRP. The level of HSPG also showed a unique trend because HSPG on the cell surface was quickly digested endocytosis, so that it was not suitable for the inflammatory index in synovial fluid with OA.
Abstract Alternative 変形性膝関節炎(OA)患者は,高齢化社会の到来とともに今後ますます増加するものと考えられる。OAの発症メカニズムについては,関節軟骨の破壊・修復やパンヌス形成に関わる多くの関連因子(サイトカイン,マトリックス分解酵素,分解酵素抑制因子,プロテオグリカン,活性酸素,細胞接着因子など)について,多面的に研究が進んでいる。しかし,膝関節の炎症初期の病態のメカニズムについては,なお詳細に解明されておらず,関節炎初期の炎症指標の検索が今後さらに必要と考えられる。本研究で私たちは, OA患者膝関節液中の細胞外マトリックス構成成分であるコラーゲン,へパラン硫酸プロテオグリカン,ウロン酸および炎症の最も初期に血管内皮細胞に出現するVCAM-1等の可溶化成分濃度を測定した。患者膝関節液(SF)中の可溶性コラーゲン量と可溶性VCAM-1(sVCAM-1)量とは有意な正の相関関係が(ρ=0.652)観察された。しかし,ヘパラン硫酸プロテオグリカン(HSPG)と可溶性コラーゲン(sCOLL)値間には有意な相関関係は確認されなかった。また,一般に炎症の指標として用いられるCRP値とも比戟検討したが,CRP値と他の測定項目値とは一定の相関関係は存在しなかった。以上の結果より,OA患者のSF中の炎症マーカ-として,急性炎症時には従来の炎症指標蛋白質であるCRP値が有力であるというSipe JDの報告から推測するとsCOLLとsVCAM-1はCRP値と一定の相関を示さないことより,CRPの動態とは異なった炎症指標としての有用性が考えられる。しかし,HSPGはエンドサイトシスにより速やかに代謝されるためSF中への解離が少なく,炎症指標としての可能性は低いと考えられる。
Keywords 変形性膝関節炎 (Osteoarthritis) 細胞外マトリックス (Extracellular matrix) 可溶性VCAM-1 (Soluble VCAM-1) 可溶性コラーゲン (Soluble Collagen) 可溶性ヘパラン硫酸プロテオグリカン (Soluble HSPG)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1997-01-31
Volume volume7
Issue issue2
Start Page 99
End Page 103
ISSN 0917-4494
language 英語
File Version publisher
NAID 120002307341
JaLCDOI 10.18926/11918
Title Alternative リウマチ因子陽性ヒト血清中の免疫グロブリンGクラスリウマチ因子と免疫複合体ならびにCRPとの相関性
FullText URL 005_071_077.pdf
Author Tohge, Hiroko| Ichimura, Mitsuko| Sakiyama, Junko| Mori, Shuji| Nakata, Yasunari| Endo, Hiroshi|
Abstract We measured the concentration of three immunoglobulin classes of rheumatoid factors (Ig-RFs), C3d binding IgG immune complex (C3d-IC), C1q binding IgG immune complex (C1q-IC) and C-reactive protein (CRP) in 74 samples of human sera with high levels of RF (24.0~2350.0IU/ml). In sera with high levels of C3d-IC (>15.0μg/ml), there was a positive correlation between the levels of CRP and the IgG-RF, but there was no correlation between the levels of CRP and the immune complexes (C3d-IC and C1q-IC). And then, there was a positive correlation between the levels of CRP and IgG-RF or C3d-IC and IgG-RF when the levels of C1q-IC in patients sera were higher than 80.0μg/ml. However, there was no correlation between the levels of CRP and C1q-IC in these patients sera containing high levels of both C3d-IC and C1q-IC. These results indicated that the determination of C3d-IC, C1q-IC, IgG-RF and CRP in human sera containing RF denote different implications as inflammatory indexes on progression of rheumatoid arthritis and other autoimmune diseases.
Abstract Alternative 一般に慢性関節リウマチの診断ならびに活動性の一指標として、日常臨床検査では患者血清中のリウマチ因子の測定が実施されている。本論文ではRF検査依頼が有り、高RF値(24.0~2350.0IU/ml)を示した患者血清74検体について、免疫グロブリンクラス別RF値を測定するとともに自己IgGと補体との免疫複合体(IC)であるC1q-IgG・IC(C1q-IC)、C3d-IgG・IC(C3d-IC)ならびにCRP値を測定し、それらの測定値間の相関性について統計学的検討を行った。C3d-IC値が15.0μg/ml以上の高値を示す患者血清では、CRP値とIgGクラスのRF(IgG-RF)値間については正の相関結果がが得られた。また、C1q-IC値が80.0μg/ml以上の場合にもCRP値とIgG-RF値間およびC3d-IC値とIgG-RF値間では正の相関結果が得られた。しかし、C3d-ICならびにC1q-ICともに高値例の患者血清中のCRP値とC1q-IC値間には有意な相関は観察されなかった。以上の結果より、慢性関節リウマチの活動度を判定する上で、従来から炎症マーカーとしてCRP値が利用されているが、血清中に免疫複合体が高レベルに検出される患者については、炎症の指標としてCRP以外にもC1q-ICやC3d-ICおよびIgG-RF等を加えた総合的な判断が必要であることが示唆される。
Keywords self-associating IgG rheumatoid factor (自己IgG結合性リウマチ因子) C1q binding IgG immune complex (C1q結合IgG免疫複合体) C3d binding IgG immune complex (C3d結合IgG免疫複合体) C-reactive protein (C反応性タンパク質(CRP))
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1995-01-31
Volume volume5
Start Page 71
End Page 77
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313402