start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=11
article-no=
start-page=2103
end-page=2116
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19561130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Virulence and Immunity of Newly Isolated Rickettsia Tsutsugamushi Strains II. Immunologicol studies of Rickettsia tsutsugamushi
kn-title=新しく分離したR. tsutsugamushi群病毒の毒性及び免疫学的研究 第二篇 R. tsutsugamushi群病毒の免疫学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In this report the proliferation of the newly isolated R. tsutsugamushi strains in the yolk sack was studied. Moreover, using the well-proliferating strain, the immunological natures were also investigated. The results are as follows: 1. Proliferation and fixation in embryonated egg yolk sack are varied according to the sorts of strains: namely two strains from rats gradually showed good naturalization and fixaiont by successive inoculation, but those from patients could not show any prolifiration 2. In infection-protective test, using the rickettsiae prolifirated in yolk sack and the antigens prepared from the infected animal organs, the immune effect of the former seemed to be inferior to that of the latter. This will be due to the difference of proliferation of rickettia. 3. In order to prepare the antigen of complement-fixation test, varioùs methods were tried, of which the method with organic solvents seemed to be relatively simple and reliable.
en-copyright=
kn-copyright=

en-aut-name=NakayamaTadao
en-aut-sei=Nakayama
en-aut-mei=Tadao
kn-aut-name=中山忠夫
kn-aut-sei=中山
kn-aut-mei=忠夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=11
article-no=
start-page=2093
end-page=2102
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19561130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Virulence and Immunity of Newly Isolated Rickettsia Tsutsugamushi Strains I. Susceptibility of mouse and embryonated egg
kn-title=新しく分離したR. tsutsugamushi群病毒の毒性及び免疫学的研究 第一篇 マウス及び孵化鶏卵に対する感受性について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The auther carried out the experiments on the susceptibility of the mouse and embryonated egg to the strains of R. tsutsugamushi newly isolated in the various areas of Kagawa prefecture of japan. where "Umayado disease" and its suspected cases had been prevailing. The results are as follows: 1. The mouse shows greater susceptibility to the R. tsutsugamushi strains isolated in Kagawa prefecture than to the other reported ones. sometimes, however, it is not always infected according to the routs of inoculation'and, among various methods, intraperitoneal and intracranial inoculation are better methods becouse these take more stable infectious course, but it is necessary to change the methods according to the aim of experiment. 2. The mortality of the mice inoculated with this R. tsutsugamushi begins to rise from fifth day of inoculation, reachs the maximum on the twelveth or fourteenth day and then falls down. The rickettsiae, however, seems to stay in the bodies for a long time. 3. The susceptibility of the embyonated egg is very specific, namely the fixation and naturalization to it ability is different according to the sorts of strains.
In the case where many riekettsiae are observable in yolk sack, LD50 is proved to be very high.
en-copyright=
kn-copyright=

en-aut-name=NakayamaTadao
en-aut-sei=Nakayama
en-aut-mei=Tadao
kn-aut-name=中山忠夫
kn-aut-sei=中山
kn-aut-mei=忠夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=11
article-no=
start-page=2083
end-page=2092
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19561130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the metabolism of Rickettsia
kn-title=Rickettsiaの代謝に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The auther tried the partial purification of rickettsiae proliferated in the embryonated egg yolk sacks, and measured the respiration of these partially purified rickettsiae by the usual manometric technique. The results are as follows: 1. By partial purification, it was possible to obtain more purified and not inactivated rickettsiae. 2. As a result of oxygen consumption measurement with Warburg manometer, glutamate, succinate and aspartate were strongly oxidized, and, at the same time, α-ketoglutarate, fumarate, malate, oxaloacetate, pyruvate and β-glycerophosphate were also oxidized pretty well. 3. All enzymic inhibitors showed some inhibition, of which most remarkable was that by monoiodoacetate. 4. The inhibitive action of various antibiotics is varied according to their concentration. However, aureomycin proved to show always very remarkable inhibition. 5. Though incomplete, Rickettsia tsutsugamushi has its proper metabolic system, and is inferred to keep its proliferation by completing its metabolic system in support or connection with living cells
en-copyright=
kn-copyright=

en-aut-name=NakayamaTadao
en-aut-sei=Nakayama
en-aut-mei=Tadao
kn-aut-name=中山忠夫
kn-aut-sei=中山
kn-aut-mei=忠夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END