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ID 17036
Eprint ID
17036
FullText URL
Thumnail 91_1579.pdf 1.3 MB
Title Alternative
Induction of erythroblasts in polycythemic rat bone marrow after acute blood letting
Author
Nakatsuka, Hisashi
Abstract
Wistar rats were made polycythemic by daily injections of massive numbers of red cells for one week. The injections were made into the peritoneal cavity and the Ht level rose to between 70 and 75%. This plethoric state resulted in suppressed erythropoiesis of the bone marrow. At this stage, a quantity of blood was drawn from the orbital sinuses with concomitant transfusion of a saline-glucose-blood serum mixture. This withdrawal-transfusion process took about 30 minutes and the Ht value decreased from 75 to 25%. Distinct proliferation of erythroblasts and basophilic erythroblasts was observed 6 to 9 hours after blood withdrawal. Polycythemic animals had many large blast-like cells having relatively large clear cytoplasm. In each of these cells, the nucleus had fine chromatin nets and an ambiguous nucleolus. At 3 hours after blood withdrawal proerythroblast like cells with basophilic cytoplasm had appeared, but there were no basophilic erythroblasts. Typical proerythroblasts having characteristic nuclear chromatin nets and deep blue cytoplasm were prominent after 6 to 9 hours. More mature, poly- and orthochromatic erythroblasts appeared between 9 and 12 hours after blood letting. At 72 hours after blood letting, the cytohistologic picture of the bone marrow of polycythemic animals had returned to nearly normal. Possible erythroid cell differentiation from precursors in the bone marrow and from circulating blood is discussed.
Keywords
多血動物
赤芽球分化
骨髄赤芽球前駆細胞
末梢血液幹細胞
Published Date
1979-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume91
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1579
End Page
1590
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/91/11-12/91_11-12_1579/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma