Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

Cytoplasmic Maturation of Porcine Oocytes for Successful Male Pronuclear Formation and Early Embryonic Development

Successful in vitro production of production of porcine embryos requires a series of integrated, effective techniques for in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). This paper reviews about cytoplasmic maturation associated with the efficiency of in vitro production of porcine embryos. Traditionally, the failure to from a male pronucleus have been reported as serious problems in producing porcine emabryos following IVM and IVF. The problem of male pronuclear formation is currently considered to be mainly due to oxidative stress during IVM. More recently the developmental competence of embryos following IVM and IVF has been investigated through improvement of culture conditions for oocyte maturation. Currently, an acceptable rate of blastocyst formation and the birth of live piglets has been achieved by investigating affecting factors during IVM, IVF and IVC of porcine oocytes. Since the ovarian oocytes available for IVM are primarily those present in mid-size antral follicles of prepuberal gilts, more research is needed to gain an improved understanding of the factors associated with the developmental competence in oocytes from both preantral and antral follicles.
developmental competence