Journal of Okayama Medical Association
Published by Okayama Medical Association

<Availability>
Full-text articles are available 3 years after publication.

ゲル電気泳動分離された高リジン型塩基性蛋白質の特異的銀染色

Nagai, Hiroshi
Thumnail 94_995.pdf 1.85 MB
Abstract
A simple silver stain for selective visualization of lysine-rich proteins in polyacrylamide gels was developed. The specificity of the stain was confirmed with a variety of protein mixtures. The staining procedure requires a relatively short time (2.5~3 hours) and has a sensitivity comparable to that of the conventional Coomassie blue stain (about 0.1μg per band for lysine-rich histones). Differences between the new staining procedure and other "highly sensitive non-specific silver stains" are discussed.
Keywords
銀染色
塩基性蛋白質
ポリアクリルアミド・ゲル電気泳動
ISSN
0030-1558
NCID
AN00032489